WormMine: ExpressionPattern Expr1042

• Pattern: MES-1 is detected as a thin crescent, initially in four-cell embryos between P2 and EMS and then in eight- to 12-cell embryos between P3 and E. MES-1 persists through the formation of P4 at the 24-cell stage. After P4 is generated, MES-1 begins to fade and is usually not detectable beyond the 30-40-cell stage. The crescent shape of MES-1 staining outlined a portion of the contact area between the germline and the gut cell; the tips of the crescent point dorsally, towards the interior of the embryo. From late prophase through anaphase, the more ventral centrosome was closely juxtaposed to the MES-1 crescent, with the two appearing to almost touch. By telophase, MES-1 and the centrosome had separated. MES-1 staining is localized in the germline cell starting with P2 (by P2 cell dissociation assay). Co-staining of MES-1 and actin shows the cytoplasmic domain of MES-1 to be on the P-cell side of the zone of germline-gut contact.
• Primary Identifier: Expr1042
• Remark: In this paper authors refer to EMS as a gut cell, though it generates more than just gut.
• Subcellular Localization: MES-1 staining was present adjacent to and sometimes overlapped with the cell cortex (marked by actin staining).

4 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Embryonic founder cell	E	lineage name: P0.pap	WBbt:0004804
embryonic founder cell	Psub3	lineage name: P0.ppp	WBbt:0006875
embryonic founder cell	Psub2	lineage name: P0.pp	WBbt:0006873
Embryonic founder cell of germ line	Psub4	lineage name: P0.pppp	WBbt:0004421

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00003219	mes-1	F54F7.5	Caenorhabditis elegans

1 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The C. elegans life stage spanning 0-100min after first cleavage at 20 Centigrade. Proliferate from 1 cell to 28 cells. From first cleavage until the start of gastrulation.		blastula embryo Ce	WBls:0000005