1 Genes
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00001649 | gob-1 | H13N06.3 | Caenorhabditis elegans |
| Pattern | The immunohistochemical detection of GOB-1 confirmed the temporal and tissue specific expression pattern shown by the transcriptional reporter. | Primary Identifier | Expr3758 |
| Remark | Despite extensive efforts varying fixation and staining conditions, endogenous GOB-1 protein could not be detected in wild-type embryos using these antibodies. Nonetheless, the antibodies could easily detect GOB-1 protein in strains transgenic for the complete gob-1 locus. Staining was specific in that it was abolished by pre-incubation of the antibody with purified GOB-1 protein. Moreover, antibody staining was (almost) completely correlated with expression of the co-injected ttx-3::GFP marker gene, i.e., GFP-expressing transgenic embryos invariably stained with the anti-GOB-1 antibody but non-transgenic embryos did not; rare exceptions could be attributed to mosaic expression of the ttx-3::GFP marker. | Subcellular Localization | The GOB-1 protein appears cytoplasmic throughout all of the stages examined although there are sporadic exceptions: rare embryos (<1%) show GOB-1 staining highly concentrated in nuclei; the significance of these observations could not be determined, and experimental conditions could not be found in which such nuclear staining was reproducible. |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00001649 | gob-1 | H13N06.3 | Caenorhabditis elegans |
