1 Genes
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00003210 | mel-28 | C38D4.3 | Caenorhabditis elegans |
| Primary Identifier | Expr12964 | Subcellular Localization | Using CRISPR-Cas9 technology, the authors generated a GFP knock-in mel-28 allele to analyze the expression of endogenous MEL-28 by live microscopy. Similar to the observations with antibodies against MEL-28, GFP::MEL-28 localized to the nuclear envelope (NE) in all cell types during embryonic and larval development and in adults. Thus, they conclude that MEL-28 is ubiquitously expressed throughout C. elegans development. MEL-28 strongly accumulated on condensed oocyte chromosomes. In the -4 oocyte, MEL-28 localized to the NE and was absent from condensed chromosomes. In the -3 and -2 oocytes MEL-28 gradually moved away from the NE and accumulated uniformly on meiotic chromosomes. Later, in the -1 oocyte MEL-28 redistributed to cover the surface of meiotic chromosomes, in some cases completely enclosing the chromosomes and in other cases similar to the 'cup-shaped' localization of kinetochore proteins, such as KNL-1 and KNL-3. The association of MEL-28 with chromosomes persisted throughout meiosis I and II until pronuclear formation ~30 minutes after germinal vesicle breakdown. During interphase full- length MEL-28 was mainly localized to the NE but was also found in the nucleoplasm. In prophase and prometaphase, MEL-28 left the NE before complete NE breakdown and associated to the condensing chromosomes. By metaphase, MEL-28 appeared as two lines parallel to the metaphase plate, resembling the characteristic pattern of holocentric kinetochore proteins, and less abundantly to the area of the mitotic spindle. During anaphase, MEL-28 associated to decondensing chromosomes, and re-localized to reforming NE in telophase. |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00003210 | mel-28 | C38D4.3 | Caenorhabditis elegans |
