|
|
Genes enriched in intestine. |
To identify genes that are significantly enriched by mRNA tagging, we first normalized the total amount of Cy3 and Cy5 signal to each other in each hybridization. We measured the ratio of the signals from the co-immunoprecipitated mRNA (Cy5) to total RNA in the cell extract (Cy3), and calculated the percentile rank for each gene relative to all genes in each hybridization. The mean percentile rank was determined from eight repeats of the mRNA-tagging experiment. Student's t-test was used to determine which genes showed a mean enrichment significantly greater than the median enrichment for all genes (P<0.001). |
WBPaper00026980:intestine_enriched
|
|
|
Genes upregulated by fer-1 mutants hc1 and hc24, with > 4 fold change in expression level. |
Data across the wild-type series was analyzed using the significance analysis of microarray data (SAM) algorithm to calculate the false discovery rate (FDR). Authors calculated expression ratios of wild-type/fer-1 mutants, and genes that exhibited a <= 0.1% FDR, >= 4-fold change in both fer-1 mutants, and differed by <= 2-fold between hc1 and hc24 were considered to be fer-1-regulated genes. |
WBPaper00035197:fer-1_upregulated
|
|
|
Genes downregulated by fer-1 mutants hc1 and hc24, with > 4 fold change in expression level. |
Data across the wild-type series was analyzed using the significance analysis of microarray data (SAM) algorithm to calculate the false discovery rate (FDR). Authors calculated expression ratios of wild-type/fer-1 mutants, and genes that exhibited a <= 0.1% FDR, >= 4-fold change in both fer-1 mutants, and differed by <= 2-fold between hc1 and hc24 were considered to be fer-1-regulated genes. |
WBPaper00035197:fer-1_downregulated
|
|
|
Genes upregulated in mes-4 relative to wild type. |
Confidence levels were determined using a two-tailed paired t-test. |
WBPaper00028483:Up_mes-4_vs_WT
|
|
|
Genes downregulated in mes-4 relative to wild type. |
Confidence levels were determined using a two-tailed paired t-test. |
WBPaper00028483:Down_mes-4_vs_WT
|
|
|
Genes enriched in muscle. |
A Student t-test was used to determine which genes showed a mean enrichment significantly greater than the mean enrichment for all genes. |
[cgc5428]:clustered_muscle_genes
|
|
|
The cluster contains genes that are significantly enriched in L1 muscle. |
A Student t-test was used to determine which genes showed a mean enrichment significantly greater than the mean enrichment for all genes. |
[cgc5428]:L1_muscle_enriched
|
