WormMine: RNAi WBRNAi00038539

• WormBase ID: WBRNAi00038539
• Genotype: sid-1(qt2)
• Phenotype Remark: Animals expressing the pcol-10::nhr-25 sense and antisense constructs in sid-1(qt-2) background sometimes had a shorter body length and were more severely defective in the posterior end.
• Remark: For tissue-specific RNAi, the first 0.9 kb of nhr-25 coding sequence was fused in the sense and antisense orientations to the col-10 promoter, which is expressed in epidermal cells during embryonic and larval stages. Both constructs were injected at 50 ng/mkl, and a sur-5::gfp transcriptional fusion construct was used as a coinjection marker.

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Data Sets

• WormBaseAcedbConverter

1 Inhibits Gene

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00003623	nhr-25	F11C1.6	Caenorhabditis elegans

2 Inhibits Predicted Gene

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:F11C1.6a	F11C1.6a	1719	X: 13008892-13009008
CDS:F11C1.6b	F11C1.6b	1479	X: 13009481-13009499

1 Laboratories

Primary Identifier
MH

3 Phenotype

Identifier	Name	Description
WBPhenotype:0000640	egg laying variant	Animals exhibit variations in the stage of eggs laid, egg laying cycle, number of eggs or egg laying in response to stimuli compared to control.
WBPhenotype:0000638	molt defect	Animals exhibit defects in the process of periodic shedding, of part or all of its cuticle, during cuticle replacement that punctuates each larval stage.
WBPhenotype:0000535	organism morphology variant	Animals exhibit variations in the form, structure or composition of any part of their anatomy compared to control animals.

0 Phenotype _ Not _ Observed

1 Reference

First Author	Title	Year	Journal	Volume	Pages	PubMed ID
						WBPaper00024419

0 Strain