WormMine: RNAi WBRNAi00106278

• WormBase ID: WBRNAi00106278
• Genotype: unc-45(e286)
• Phenotype Remark: "RNAi knockdown of chaperones was next performed with unc-45(ts) mutant strains under permissive conditions. Age synchronized L1 unc-45(e286) mutant animals kept at permissive conditions (15C) were treated with RNAi for different molecular chaperones and monitored for motility defects. We first sought chaperones that cause unc-45(e286)-specific phenotypes at permissive conditions when depleted (Figure 2A). Of the 96 RNAi constructs tested, five genes induced loss of coordination and/or an egg-laying defect, resulting in reduced motility in more than 70% of the unc-45(e286) mutant animals. As noted above, down-regulation of unc-45 or unc-23 also induced a loss of coordination in wild-type animals (Table 1); these were discarded from the candidate gene list (Figure 2A). The remaining chaperones identified in the screen are CeHsp90 (daf-21), CeHop (sti-1) and CeAha1 (C01G10.8) (Figures 2B,C)." "RNAi treatment for CeHsp90, encoding a known myosin chaperone, resulted in a strong larval arrest phenotype in both wild-type and unc45(e286) animals but induced a strong motility defect only in unc-45(e286) animals (76.4 8.3%, as compared to 12.1 4% motile animals)."
• Remark: (Figure 2) daf-21/CeHsp90 RNAi. Exact sequence used for RNAi not stated by authors, Ahringer laboratory clone used for curation. If Ahringer clone not available, Vidal laboratory clone used for curation.

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Data Sets

• WormBaseAcedbConverter

2 Inhibits Gene

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000915	hsp-90	C47E8.5	Caenorhabditis elegans
WBGene00044180	C47E8.11	C47E8.11	Caenorhabditis elegans

2 Inhibits Predicted Gene

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:C47E8.5	C47E8.5	2109	V: 14686164-14686313
CDS:C47E8.11	C47E8.11	237	V: 14688637-14688711

0 Laboratories

2 Phenotype

Identifier	Name	Description
WBPhenotype:0000644	paralyzed	Animals do not move and are not responsive to external mechanical stimulation.
WBPhenotype:0000059	larval arrest	The developmental program of the animals halts at any larval stage and thus fails to reach adulthood. In C.elegans, this occurs anytime during L1-L4 stage.

0 Phenotype _ Not _ Observed

1 Reference

First Author	Title	Year	Journal	Volume	Pages	PubMed ID
						WBPaper00046852

0 Strain