WormMine: RNAi WBRNAi00088823

• WormBase ID: WBRNAi00088823
• Genotype: [GFP::PAR-6]
• Phenotype Remark: "To determine whether the appropriate localization of the anterior PAR complex also relied on Arp2/3-mediated actin dynamics, we examined GFP-PAR-6 localization in control and ARX-2-depleted embryos. As previously reported, GFPxPAR-6 localizes to the anterior cortex during the establishment phase and is maintained there (Munro et_al, 2004). In the arx-2 RNAi-treated embryos, the cortical area occupied by GFP-PAR-6 was somewhat reduced during the maintenance phase; however, this was statistically significant only at the PN (pronuclei) meeting + 1:00 time point (Figure 1, G and H), further suggesting that disruption of Arp2/3 leads to a destabilization of PAR asymmetry specifically during the maintenance phase... We also observed GFP-PAR-6-labeled foci at or near the anterior cortex during the establishment phase and earlier portions of the maintenance phase in wild-type embryos (Figure 1G, +4:00). The nature of these puncta is unclear; however, they appear to disperse or are removed from the cortex by 4:00 after pronuclear meeting. In embryos depleted of ARX-2, these foci appear to remain at or near the cortex longer than what was observed in wild-type embryos (Figure 1G, +4:00) and formed large aggregates similar to those observed in the moe-GFP background (Figure 1A)."
• Remark: (Figure 1G,1H) arx-2 RNAi

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Data Sets

• WormBaseAcedbConverter

1 Inhibits Gene

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000200	arx-2	K07C5.1	Caenorhabditis elegans

1 Inhibits Predicted Gene

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:K07C5.1	K07C5.1	1188	V: 10335776-10335949

1 Laboratories

Primary Identifier
MAD

1 Phenotype

Identifier	Name	Description
WBPhenotype:0000679	transgene subcellular localization variant	Any variation in the subcellular localization of a gene product produced by DNA coded for by exogenous genetic material that has been introduced into the organism compared to its expression in control animals.

0 Phenotype _ Not _ Observed

1 Reference

First Author	Title	Year	Journal	Volume	Pages	PubMed ID
						WBPaper00040933

0 Strain