WormMine: RNAi WBRNAi00087569

• WormBase ID: WBRNAi00087569
• Genotype: itIs153[pie-1::GFP::PAR-2]
• Phenotype Remark: GFP:PAR-2 was asymmetric in most mex-5(RNAi) mex-6(RNAi) zygotes (5/7). However, GFP:PAR-2 dynamics were clearly aberrant in those embryos. In wild type, the GFP:PAR-2 domain expands quickly and reaches its maximal domain (close to 50% egg length) by pseudocleavage. In contrast, in five out of seven mex-5(RNAi)mex-6(RNAi) embryos, the GFP:PAR-2 domain remained small (33% egg length) throughout pronuclear migration and began to expand only after pronuclear meeting, eventually reaching its normal distribution just before the first cleavage. This pattern was essentially opposite that seen in par-1(RNAi) embryos, where the PAR-2 domain extends further than normal. In the other 2 embryos examined (2/7), PAR-2:GFP was never seen at the cortex. Unlike most mex-5(RNAi);mex-6(RNAi) embryos, which divide asymmetrically, these two embryos divided symmetrically.
• Remark: paper remark: Exact sequence used for RNAi not stated by authors, spliced coding region sequence of gene used for curation.

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Data Sets

• WormBaseAcedbConverter

2 Inhibits Gene

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00003230	mex-5	W02A2.7	Caenorhabditis elegans
WBGene00003231	mex-6	AH6.5	Caenorhabditis elegans

2 Inhibits Predicted Gene

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:AH6.5	AH6.5	1404	II: 9524181-9524309
CDS:W02A2.7	W02A2.7	1407	IV: 13353730-13353843

1 Laboratories

Primary Identifier
JH

1 Phenotype

Identifier	Name	Description
WBPhenotype:0000961	pattern of transgene expression variant	Any variation in the cellular or subcellular pattern of expression of a gene product produced by exogenous DNA that has been introduced into the organism compared to its expression in control animals.

0 Phenotype _ Not _ Observed

1 Reference

First Author	Title	Year	Journal	Volume	Pages	PubMed ID
						WBPaper00005735

0 Strain