WormMine: RNAi WBRNAi00092412

• WormBase ID: WBRNAi00092412
• Phenotype Remark: tfg-1 RNAi appeared to disrupt the formation of a SEC-16-containing protein complex and a SEC-13-containing protein complex, as determined by Superose 6 gel filtration followed by Western blot of the filtration fractions. The SEC-16 complex appeared to be reduced in size from a Stokes radius of about 125 Angstroms to about 55 Angstroms. Similarly, the SEC-13 complex appeared to be reduced to monomeric SEC-13, as the Stokes radius was reduced from 128 Angstroms to 24 Angstroms.
• Remark: (Figure 4e) tfg-1 RNAi. Authors report testing three different dsRNA sequences to knockdown tfg-1 and all produced similar results. One selected for curation.

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Data Sets

• WormBaseAcedbConverter

1 Inhibits Gene

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00006565	tfg-1	Y63D3A.5	Caenorhabditis elegans

1 Inhibits Predicted Gene

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:Y63D3A.5	Y63D3A.5	1461	I: 14102047-14102227

1 Laboratories

Primary Identifier
MSN

1 Phenotype

Identifier	Name	Description
WBPhenotype:0002132	protein complex organization variant	The formation, organization, or stability of a protein complex is altered in comparison to corresponding complexes in control animals.

0 Phenotype _ Not _ Observed

1 Reference

First Author	Title	Year	Journal	Volume	Pages	PubMed ID
						WBPaper00038310

0 Strain