WormMine: RNAi WBRNAi00110366

• WormBase ID: WBRNAi00110366
• Phenotype Remark: "Prominent cellular events in the wild-type zygote include formation of the perivitelline space and vigorous movement of plasma membrane during ruffling, pseudocleavage, and furrowing (Fig. 3A-E). Strikingly, none of these post-fertilization hallmarks were observed in either acs-1(RNAi)C17ISO or embryos from acs-1(gk3066);Ex[acs-1(full)::GFP] animals that lost acs-1 expression in the somatic gonad. Instead, the cytoplasm of these embryos appeared to occupy the entire egg (i.e., egg-filling phenotype) without any sign of wild-type membrane dynamics (Fig. 3F-I)... In other embryos, cellular cleavage reached completion but enclosed more than one nucleus within a single daughter cell (Fig. 3I,K,L). As a result, these embryos displayed the Emb phenotype with characteristic formation of multinucleated cells and accumulation of peculiar membranous structures in the cytoplasm (Figs. 3I-L, 4F)... Electron microscopy (EM) analysis of dividing blastomeres in acs-1(RNAi)C17ISO embryos confirmed instances of unusual membrane formation, revealing membrane branching, multiple breaks, and the presence of various membranous shapes alongside the cleavage furrow (Fig. 3M-O)... Moreover, the failure of cleavage in acs-1(RNAi)C17ISO embryos did not always occur during the very first cell division, and cleavage failure in subsequent cell divisions was always accompanied by erratic membrane formations (Figs. 3K,L,N,O, 4F)."
• Remark: (Figure 3, 4F) acs-1(RNAi)

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Data Sets

• WormBaseAcedbConverter

1 Inhibits Gene

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00018488	acs-1	F46E10.1	Caenorhabditis elegans

3 Inhibits Predicted Gene

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:F46E10.1c	F46E10.1c	1737	V: 6529269-6529439
CDS:F46E10.1a	F46E10.1a	1872	V: 6525095-6525136
CDS:F46E10.1b	F46E10.1b	1794	V: 6527511-6527520

0 Laboratories

1 Phenotype

Identifier	Name	Description
WBPhenotype:0001399	cell membrane morphology variant	Any variation in the structure, form or composition of the semipermeable lipid bilayer that creates a boundary between the interior and exterior environment for cells, cellular organelles and tissues, within an organism compared to control.

0 Phenotype _ Not _ Observed

1 Reference

First Author	Title	Year	Journal	Volume	Pages	PubMed ID
						WBPaper00040893

0 Strain