WormMine: RNAi WBRNAi00101780

• WormBase ID: WBRNAi00101780
• Genotype: jjIs1475(myo-3::rfp) I; rrf-3(pk1426) II; ccIs4438 (intrinsic CC(CoelomoCyte)::gfp) III; ayIs2(egl-15::gfp) IV; ayIs6(hlh-8::gfp) X
• Phenotype Remark: Postembryonic RNAi targeting ceh-34 resulted in adult animals lacking the two posterior (M-lineage derived) coelomocytes (CCs), as indicated by loss of expression of the 'intrinsic CC::gfp' coelomocyte marker in the posterior of the adult animal. The M lineage was followed in these animals using the hlh-8::gfp reporter and anti-FOZI-1 immunostaining, and normal cleavage orientations and proliferation were observed in the M lineage. However, at the 18-M stage, both M.drpa and M.dlpa were transformed from CCs to body wall muscles (BWMs; as observed using myo-3::rfp) in 10 of 11 animals examined, while the remaining animal had only one CC transformed to a BWM (data not shown).
• Remark: (Figure 1B,D, Table 1) ceh-34 RNAi-P (postembryonic RNAi)

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Data Sets

• WormBaseAcedbConverter

1 Inhibits Gene

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000455	ceh-34	C10G8.6	Caenorhabditis elegans

1 Inhibits Predicted Gene

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:C10G8.6	C10G8.6	771	V: 5315694-5315874

1 Laboratories

Primary Identifier
LW

5 Phenotype

Identifier	Name	Description
WBPhenotype:0000095	M lineage variant	The descendants of the M precursor cell exhibit variations in developmental programs compared to their counterparts in control animals. In C. elegans the M lineage is a postembryonic mesodermal lineage.
WBPhenotype:0000961	pattern of transgene expression variant	Any variation in the cellular or subcellular pattern of expression of a gene product produced by exogenous DNA that has been introduced into the organism compared to its expression in control animals.
WBPhenotype:0000414	cell fate transformation	The cell takes on a different identity from which it was initially committed.
WBPhenotype:0000852	coelomocyte development variant	Variations in the progression of a free-floating spherical cell lying in the pseudocoelomic cavity of animals that can endocytose many compounds, possibly for immune surveillance, over time from an initial condition to a later condition compared to control animals.
WBPhenotype:0001912	fewer coelomocytes	The number of coelomocytes are reduced compared to control animals. Coelomocytes are free-floating spherical cells lying in the pseudocoelomic cavity of animals that can endocytose many compounds.

1 Phenotype _ Not _ Observed

Identifier	Name	Description
WBPhenotype:0000669	sex muscle development variant	Variations in the progression of the muscles of the adult reproductive system over time form their initial formation to their mature structure compared to control.

1 Reference

First Author	Title	Year	Journal	Volume	Pages	PubMed ID
						WBPaper00033137

0 Strain