WormMine

WS294

Intermine data mining platform for C. elegans and related nematodes

RNAi :

WormBase ID  WBRNAi00087542 Phenotype Remark  Several developmental defects were observed in treated animals. bir-1 RNAi resulted in an egg-laying (Egl) phenotype in 80% of young adult animals (n = 3,408) that became nearly fully penetrant in older adults. bir-1 RNAi-treated hermaphrodites laid 50% the normal number of embryos compared with control animals (n = 20 for each group). The vulva and egg-laying muscles were formed normally suggesting a neuronal defect as the cause of the Egl phenotype in these bir-1 RNAi adults. A short and fat dumpy (Dpy) phenotype was also observed in a fraction (n = 992, 29%) of larvae at L3 and L4 stage and young adult animals when L1 larvae were treated with bir-1 RNAi. If larvae L2 were treated with bir-1 RNAi, the effect was less pronounced and visible later during adulthood (n = 208, 26%). In animals showing the pronounced Dpy phenotype the movement was also affected. The worms showed slow uncoordinated (Unc) movement. Non-Dpy animals also had movement defects after bir-1 RNAi that consisted of larger-than-normal amplitudes of body curvature. There was also a germ-line defect in 20% of bir-1 RNAi-treated animals (n = 680). Wild-type adult hermaphrodites have two germ-line-filled gonad arms arranged symmetrically above the vulva. During the L2 to L4 larval stages, the gonads form by elongation of the germ-line and somatic gonad primordium. At the leading edge of each elongating gonad arm is a distal tip cell (DTC), the migration path of which dictates the shape of the gonad. After the L3 molt, the DTCs U-turn and continue to grow in opposite directions, resulting in two symmetrically reflexed gonads. The gonads in bir-1 RNAi-treated animals often made multiple turns (usually three) during elongation. The elongation defect appeared to be stochastic, and the growth of only one gonad arm was affected in any given animal. Cells composing egg laying and anal muscles were normally developed and observed on transgenic lines expressing CeSKIP::gfp. Interestingly, the DTCs did not have defects in cell division as might be expected from the known BIR-1 function in cytokinesis, suggesting that only a DTC migration defect was responsible for the abnormal gonads. There were two additional defects observed in bir-1 RNAi-treated animals that were present less frequently. In 10% (n = 344) of bir-1 RNAi-treated animals, there was an intestinal defect consisting of gut distention. A similar percentage of animals had a protruding vulva (Pvul) phenotype. bir-1 RNAi negatives affects transgene expression and endogenous gene expression.

1 Organism

Name Taxon Id
Caenorhabditis elegans 6239

1 Data Sets

Name URL
WormBaseAcedbConverter  

1 Inhibits Gene

WormBase Gene ID Gene Name Sequence Name Organism
WBGene00000249 bir-1 T27F2.3 Caenorhabditis elegans

1 Inhibits Predicted Gene

WormMine ID Sequence Name Length (nt) Chromosome Location
CDS:T27F2.3 T27F2.3 468   V: 11644763-11644885

0 Laboratories

8 Phenotype

Identifier Name Description
WBPhenotype:0000643 locomotion variant Animals exhibit deviations in self-propelled movement on a solid medium compared to control animals.
WBPhenotype:0000697 protruding vulva Animals undergo incomplete vulval morphogenesis, resulting in the formation of a single protrusion at the site of the vulva.
WBPhenotype:0001278 transgene expression reduced Any decrease in the intensity of expression of a gene product produced by exogenous DNA that has been introduced into the organism compared to its expression in control animals.
WBPhenotype:0000006 egg laying defective Eggs are not laid, laid at a slower rate or eggs are laid at a later stage of development compared to control.
WBPhenotype:0000583 dumpy Worms are shorter and stouter than control animals at the same developmental stage.
WBPhenotype:0000134 gene expression level reduced Animals exhibit a decrease in the intensity of expression of a gene product compared to that observed in control animals.
WBPhenotype:0000188 gonad arm morphology variant Any variation in the 'arm-like' form, structure or composition of the specialized tissue of the reproductive tract that produces gametes compared to control. In C. elegans the reproductive tract in the hermaphrodite has two equivalent gonad 'arms', while the male has a one-armed gonad.
WBPhenotype:0000710 intestinal morphology variant Animals exhibit variations in the form, structure or composition of the chain of cuboidal cells which form a central lumen lined by microvilli that allow the passage of food, compared to control. Food passes from the posterior pharynx to the intestine where it is digested and then on to the rectum which processes the waste products for excretion (Wormatlas).

0 Phenotype _ Not _ Observed

1 Reference

First Author Title Year Journal Volume Pages PubMed ID
            WBPaper00005853

0 Strain