WormMine: RNAi WBRNAi00086809

• WormBase ID: WBRNAi00086809
• Phenotype Remark: Injection led to a severe reduction in the fertility of the derived F1 progeny: 47% of F1 animals were completely sterile, whereas an additional 39% had brood sizes of < 10 (n = 210). An analysis of affected animals revealed pleiotropic defects within the germ-line and somatic gonad. These included a failure of gonad arms to elongate, missing or abnormal oocytes and sperm, and morphogenetic defects of the uterus and vulva (Fig. 5B).From these results, we conclude that FZR-1 function is required for viability and that ku298 encodes a hypomorphic allele of fzr-1.
• Remark: paper remark: Exact sequence used for RNAi not stated by authors, authors said fzr-1 750-bp genomic spanning exons 12 was used. Curator used 852 bp of fzr-1 genomic sequence (spanning exon 1-2) in WS227.

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Data Sets

• WormBaseAcedbConverter

1 Inhibits Gene

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00001510	fzr-1	ZK1307.6	Caenorhabditis elegans

2 Inhibits Predicted Gene

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:ZK1307.6a	ZK1307.6a	2109	II: 9640995-9641204
CDS:ZK1307.6b	ZK1307.6b	2115	II: 9640995-9641210

1 Laboratories

Primary Identifier
MH

7 Phenotype

Identifier	Name	Description
WBPhenotype:0000684	fewer germ cells	Germ cell populations are reduced compared to control animals.
WBPhenotype:0000399	somatic gonad development variant	Any variation in the progression of the components of the gonad that are separate from the germline proper over time from its formation to its mature structure compared to control (Wormatlas).
WBPhenotype:0000900	germ cell morphology variant	Any variation in the morphological appearance of germ cells compared to control animals.
WBPhenotype:0000695	vulva morphology variant	Animals exhibit variations in the gross structure or organization of the reproductive organ required for depositing eggs and for influencing male mating behavior compared to control.
WBPhenotype:0000289	uterus morphology variant	Any variation in the form, structure or composition of the portion of the somatic gonad which encloses the fertilized eggs after their passage through the spermatheca compared to control (Wormatlas).
WBPhenotype:0000188	gonad arm morphology variant	Any variation in the 'arm-like' form, structure or composition of the specialized tissue of the reproductive tract that produces gametes compared to control. In C. elegans the reproductive tract in the hermaphrodite has two equivalent gonad 'arms', while the male has a one-armed gonad.
WBPhenotype:0000694	hermaphrodite sterile	Hermaphrodites fail to generate viable self-progeny and/or cross-progeny.

0 Phenotype _ Not _ Observed

1 Reference

First Author	Title	Year	Journal	Volume	Pages	PubMed ID
						WBPaper00005137

1 Strain

WormBase ID
WBStrain00000001