WormMine: RNAi WBRNAi00088416

• WormBase ID: WBRNAi00088416
• Phenotype Remark: F1 embryos exhibited embryonic lethality with complete penetrance. Authors carefully observed the cell division process in scc-1/coh-2(RNAi) embryos, and found similar chromosome segregation defects in early cell cycles to the scc-3(RNAi), him-1/smc-1(RNAi), or smc-3(RNAi) embryos, although the penetrance was relatively low. This low penetrance (~10% of the RNAi-affected embryos) might be due to either gene-specific inefficiency of RNAi reaction or relatively high stability of the SCC-1/COH-2 protein compared with other cohesin components. The scc-1/coh-2(RNAi) embryos showed loose metaphase plates, asynchronous separation of chromosomes at anaphase, and formation of multiple nuclei after decondensation of chromosomes.
• Remark: paper remark: Exact sequence used for RNAi not stated by authors, spliced coding region sequence of gene used for curation. Authors used yk256h5(scc-1/coh-2).

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Data Sets

• WormBaseAcedbConverter

1 Inhibits Gene

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00004737	scc-1	F10G7.4	Caenorhabditis elegans

1 Inhibits Predicted Gene

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:F10G7.4	F10G7.4	1938	II: 4698721-4698972

1 Laboratories

Primary Identifier
YM

5 Phenotype

Identifier	Name	Description
WBPhenotype:0000688	sterile	Animals generate defective gametes, are otherwise unable to reproduce or they generate progeny that are unable to reproduce.
WBPhenotype:0000697	protruding vulva	Animals undergo incomplete vulval morphogenesis, resulting in the formation of a single protrusion at the site of the vulva.
WBPhenotype:0000050	embryonic lethal	Animals die during embryonic development. In C. elegans, often assayed as refractile eggs that fail to hatch; when applied to large-scale RNAi screens in C. elegans, more than 10% of embryos die.
WBPhenotype:0000059	larval arrest	The developmental program of the animals halts at any larval stage and thus fails to reach adulthood. In C.elegans, this occurs anytime during L1-L4 stage.
WBPhenotype:0000773	chromosome segregation variant	Any variation in the processes that regulate the apportionment of chromosomes to each of two daughter cells compared to control.

0 Phenotype _ Not _ Observed

1 Reference

First Author	Title	Year	Journal	Volume	Pages	PubMed ID
						WBPaper00005937

1 Strain

WormBase ID
WBStrain00000001