WormMine

WS294

Intermine data mining platform for C. elegans and related nematodes

RNAi :

WormBase ID  WBRNAi00086897 Phenotype Remark  91 to 100% of the embryos failed to hatch. Embryos depleted of IMB-1 showed a strong defect in GFP::EMR-1 recruitment to the NE-like structures after mitosis (3 of 3 embryos). Depletion of IMB-1 from embryos by RNAi give rise to a strong spindle defect like those seen in embryos from ima-2(ok256) worms and in Ran(RNAi) embryos. The centrosomes lose their association with the sperm pronucleus early and move far apart. The pronuclei never meet. The oocyte pronucleus partly aligns between the centrosomes but spindle assembly does not occur. imb-1(RNAi) embryos showed clustering of GFP::EMR-1 at the centrosomes. In addition, nuclear exclusion of soluble GFP::beta-tubulin was not observed in embryos depleted of IMB-1 (3 of 3 embryos). Immunofluorescence analysis demonstrated that chromatin in imb-1(RNAi) embryos is most often not surrounded by a smooth and continuous nucleoporin staining.
Remark  Two different C. elegans importin fragments (imb-1, nt 916 to 1425 and 1659 to 2340 of predicted ORF) were used for RNAi. Except for the 3 imb-1 fragment. only intron-less sequences were amplified.
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1 Organism

Name Taxon Id
Caenorhabditis elegans 6239

1 Data Sets

Name URL
WormBaseAcedbConverter  

1 Inhibits Gene

WormBase Gene ID Gene Name Sequence Name Organism
WBGene00002075 imb-1 F28B3.8 Caenorhabditis elegans

1 Inhibits Predicted Gene

WormMine ID Sequence Name Length (nt) Chromosome Location
CDS:F28B3.8 F28B3.8 2691   I: 4921403-4921444

0 Laboratories

4 Phenotype

Identifier Name Description
WBPhenotype:0000154 reduced brood size The characteristic number of offspring produced is lower compared to that of control animals.
WBPhenotype:0000062 lethal Animals die prematurely at any stage of the life cycle.
WBPhenotype:0001151 pronucleus centrosomes defective early emb Pronucleus is abnormal in the inviable embryo.
WBPhenotype:0000759 spindle defective early emb Early embryos exhibit defects in the assembly, disassembly, arrangement, elongation or stabilization of the microtubules and associated molecules that form between opposite poles of a eukaryotic cell during a cell cycle.

0 Phenotype _ Not _ Observed

1 Reference

First Author Title Year Journal Volume Pages PubMed ID
            WBPaper00005637

1 Strain

WormBase ID
WBStrain00000001