WormMine: RNAi WBRNAi00086896

• WormBase ID: WBRNAi00086896
• Phenotype Remark: 91 to 100% of the embryos failed to hatch. ima-3(RNAi) embryos were significantly reduced in size. However, spindle formation seemed relative normal as judged from the presence of a metaphase plate and DIC images. Nuclei were formed in ima-3(RNAi) embryos upon cell division and grew to nearly normal size, illustrating that IMA-3 is not required for nuclear formation in early embryos.
• Remark: ima-3, nt 745 to 1542 of predicted ORF was used for RNAi. Only intron-less sequences were amplified.

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Data Sets

• WormBaseAcedbConverter

1 Inhibits Gene

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00002074	ima-3	F32E10.4	Caenorhabditis elegans

1 Inhibits Predicted Gene

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:F32E10.4	F32E10.4	1545	IV: 7559791-7559899

0 Laboratories

3 Phenotype

Identifier	Name	Description
WBPhenotype:0000154	reduced brood size	The characteristic number of offspring produced is lower compared to that of control animals.
WBPhenotype:0000062	lethal	Animals die prematurely at any stage of the life cycle.
WBPhenotype:0001792	nuclei small	Nuclei of specific cells are smaller compared to control animals.

1 Phenotype _ Not _ Observed

Identifier	Name	Description
WBPhenotype:0000759	spindle defective early emb	Early embryos exhibit defects in the assembly, disassembly, arrangement, elongation or stabilization of the microtubules and associated molecules that form between opposite poles of a eukaryotic cell during a cell cycle.

1 Reference

First Author	Title	Year	Journal	Volume	Pages	PubMed ID
						WBPaper00005637

1 Strain

WormBase ID
WBStrain00000001