WormMine: RNAi WBRNAi00088423

• WormBase ID: WBRNAi00088423
• Phenotype Remark: RNAi of gpr-1 and gpr-2 results in 100% embryonic lethality. In gpr-1/2(RNAi) embryos, as in Galpha(RNAi) embryos, the first mitotic spindle fails to rock as it elongates and is not displaced posteriorly, resulting in a symmetric, rather than an asymmetric, first cleavage. In addition, spindle morphology is symmetric, with both the anterior and posterior asters having a round morphology similar to a wild-type anterior one. Despite the fact that the first cleavage is symmetric in size as in par mutants, polarity appears to be normal; polarity markers such as PAR-2, PAR-3, PAR-6, and P granules are correctly localized in these embryos.
• Remark: paper remark: Exact sequence used for RNAi not stated by authors, spliced coding region sequence of gene used for curation.

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Data Sets

• WormBaseAcedbConverter

2 Inhibits Gene

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00001688	gpr-1	F22B7.13	Caenorhabditis elegans
WBGene00001689	gpr-2	C38C10.4	Caenorhabditis elegans

2 Inhibits Predicted Gene

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:F22B7.13	F22B7.13	1578	III: 8628957-8629204
CDS:C38C10.4	C38C10.4	1578	III: 9391409-9391629

1 Laboratories

Primary Identifier
JA

2 Phenotype

Identifier	Name	Description
WBPhenotype:0000050	embryonic lethal	Animals die during embryonic development. In C. elegans, often assayed as refractile eggs that fail to hatch; when applied to large-scale RNAi screens in C. elegans, more than 10% of embryos die.
WBPhenotype:0000748	asymmetric cell division defective early emb	Symmetric (PAR-like) divisions or excessive posterior displacement (zyg-8 like phenotypes).

0 Phenotype _ Not _ Observed

1 Reference

First Author	Title	Year	Journal	Volume	Pages	PubMed ID
						WBPaper00005945

0 Strain