WormMine: RNAi WBRNAi00087516

• WormBase ID: WBRNAi00087516
• Phenotype Remark: While the uteri of wild-type control hermaphrodites were filled with rapidly dividing embryos of many different ages, the uteri of hermaphrodites that had been injected with double stranded Ce-cdc-27 RNA were filled with one-cell embryos. These cdc-27(RNAi) embryos looked like those from mat-1 mothers when examined either in utero or as individual fixed specimens that had been processed for DAPI staining and tubulin immunofluorescence. The mat-1 and cdc-27(RNAi) embryos accumulated in an arrested but otherwise normal metaphase I state. In all cases, the oocyte chromosomes were aligned in a pentagonal metaphase plate on a barrel-shaped meiotic spindle, and the sperm chromosomes remained highly condensed. Such embryos also lacked polar bodies (the discarded products of the meiotic divisions) and had incompletely hardened eggshells.
• Remark: paper remark: Exact sequence used for RNAi not stated by authors, spliced coding region sequence of gene used for curation.

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Data Sets

• WormBaseAcedbConverter

1 Inhibits Gene

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00003132	mat-1	Y110A7A.17	Caenorhabditis elegans

2 Inhibits Predicted Gene

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:Y110A7A.17a	Y110A7A.17a	2367	I: 5124192-5124346
CDS:Y110A7A.17b	Y110A7A.17b	2373	I: 5124192-5124346

1 Laboratories

Primary Identifier
AG

2 Phenotype

Identifier	Name	Description
WBPhenotype:0001100	early embryonic lethal	Animal dies prior to the gastrulation stage of embryogenesis.
WBPhenotype:0000372	no polar body formation	Animals lack the two small cells produced, each during one of the two meiotic cell divisions (Wormatlas).

0 Phenotype _ Not _ Observed

1 Reference

First Author	Title	Year	Journal	Volume	Pages	PubMed ID
						WBPaper00005823

1 Strain

WormBase ID
WBStrain00000001