Survival value (1 - % embryonic lethality) of the combination of RNAi and mutation is not significantly different (P=0.249627>0.001) from the survival values of the RNAi in wild type (lethality=1%) and the mutation without RNAi (lethality=6%), indicating the absence of genetic interaction. t-test null hypothesis: survival of the double disruption (mutation and RNAi) equals the product of survivals for each single disruption (mutation alone RNAi alone).
Animals die during embryonic development. In C. elegans, often assayed as refractile eggs that fail to hatch; when applied to large-scale RNAi screens in C. elegans, more than 10% of embryos die.