WormMine: RNAi WBRNAi00088408

• WormBase ID: WBRNAi00088408
• Phenotype Remark: In wild-type one-cell stage embryos, the spindle elongates asymmetrically toward the posterior during anaphase, accompanied by vigorous transverse movements of the posterior spindle pole, which flattens at telophase. As a result, the first division is unequal, generating a larger anterior blastomere and a smaller posterior one. In gpr-1/2(RNAi) one-cell stage embryos, the spindle did not elongate asymmetrically during anaphase in 25 of 30 cases. As a result, the first division was equal, generating two blastomeres of identical size. In the other five gpr-1/2(RNAi) embryos, the posterior spindle pole underwent weak posterior displacement, which, although clearly distinguishable from the wild type, resulted in unequal first division. In all gpr-1/2(RNAi) embryos, transverse movements of the posterior spindle pole and its flattening at telophase did not take place. Other defects were apparent when gpr-1 and gpr-2 were inactivated by RNAi, including aberrant centrosome and spindle positioning in two-cell stage embryos as well as occasional chromosome segregation defects.
• Remark: paper remark: Exact sequence used for RNAi not stated by authors, spliced coding region sequence of gpr-1 used for curation.

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Data Sets

• WormBaseAcedbConverter

2 Inhibits Gene

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00001688	gpr-1	F22B7.13	Caenorhabditis elegans
WBGene00001689	gpr-2	C38C10.4	Caenorhabditis elegans

2 Inhibits Predicted Gene

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:F22B7.13	F22B7.13	1578	III: 8628957-8629204
CDS:C38C10.4	C38C10.4	1578	III: 9391409-9391629

1 Laboratories

Primary Identifier
GZ

1 Phenotype

Identifier	Name	Description
WBPhenotype:0001959	spindle pole pulling force variant	Pulling forces on the astral microtubules at the spindle poles vary from that observed for control spindles.

0 Phenotype _ Not _ Observed

1 Reference

First Author	Title	Year	Journal	Volume	Pages	PubMed ID
						WBPaper00005918

1 Strain

WormBase ID
WBStrain00000001