WormMine: Gene WBGene00000445

• WormBase Gene ID: WBGene00000445
• Gene Name: ceh-22
• Sequence Name: F29F11.5
• Brief Description: ceh-22 encodes an NK-2 family homeodomain factor homologous to Drosophila TINMAN and vertebrate Nkx2-5 proteins; CEH-22 activates pharyngeal muscle gene expression in combination with PHA-1 and is required for normal pharyngeal development; ceh-22 is the first gene known to be expressed in pharyngeal muscles, being exclusively active in that tissue.
• Organism: Caenorhabditis elegans
• Automated Description: Enables sequence-specific DNA binding activity. Involved in several processes, including chordate pharyngeal muscle development; gonad morphogenesis; and positive regulation of transcription by RNA polymerase II. Located in nucleus. Expressed in several structures, including G1; gonad; neurons; pharyngeal cell; and ventral nerve cord. Used to study cancer. Is an ortholog of human NKX2-2 (NK2 homeobox 2).
• Biotype: SO:0001217
• Genetic Position: V :2.70908 ±0.00321
• Length (nt): 3852

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Synonyms

• Value: WBGene00000445

Genomics

5 Transcripts

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
Transcript:F29F11.5a.1	F29F11.5a.1	2009	V: 10671918-10675644
Transcript:F29F11.5d.1	F29F11.5d.1	1140	V: 10672634-10675392
Transcript:F29F11.5c.1	F29F11.5c.1	1042	V: 10673871-10675582
Transcript:F29F11.5e.1	F29F11.5e.1	1239	V: 10674232-10675769
Transcript:F29F11.5b.1	F29F11.5b.1	648	V: 10674504-10675392

Other

5 CDSs

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:F29F11.5a	F29F11.5a	1041	V: 10672634-10672845
CDS:F29F11.5b	F29F11.5b	648	V: 10674504-10674595
CDS:F29F11.5c	F29F11.5c	852	V: 10673871-10673979
CDS:F29F11.5d	F29F11.5d	1140	V: 10672634-10672845
CDS:F29F11.5e	F29F11.5e	783	V: 10674311-10674440

8 RNAi Result

• WBRNAi00045934
• WBRNAi00014111
• WBRNAi00031582
• WBRNAi00071562
• WBRNAi00115657
• WBRNAi00025316
• WBRNAi00027310
• WBRNAi00069884

54 Allele

• gk963301
• gk964451
• gk964452
• gk963495
• gk963496
• gk964351
• gk964304
• q632
• WBVar01865092
• WBVar01865093
• WBVar01865090
• WBVar01865091
• WBVar01865096
• WBVar01865097
• WBVar01865094
• WBVar01865095
• tm428
• WBVar01825829
• ttTi20523
• WBVar00002065
• WBVar00002070
• otn3445
• gk709050
• cc8266
• gk505620
• gk917209
• gk674027
• gk498679
• gk585358
• gk914350

1 Chromosome

• WormBase ID: V
• Organism: Caenorhabditis elegans
• Length (nt): 20924180

1 Chromosome Location

• Feature . Primary Identifier: WBGene00000445
• Start: 10671918
• End: 10675769
• Strand: 1

4 Data Sets

• WormBaseAcedbConverter
• GO Annotation data set
• C. elegans genomic annotations (GFF3 Gene)
• Panther orthologue and paralogue predictions

1 Downstream Intergenic Region

• WormBase ID: intergenic_region_chrV_10675770..10677667
• Length (nt): 1898
• Chromosome Location: V: 10675770-10677667
• Organism: Caenorhabditis elegans

134 Expression Clusters

Regulated By Treatment	Description	Algorithm	Primary Identifier
	Transcripts of coding genes that showed significantly decreased expression in muscle.	DESeq2 (version 1.24.0). Transcripts with a false-discovery rate adjusted p-value less than 0.05 were considered significantly differentially expressed.	WBPaper00062325:muscle_depleted_coding-RNA
	Transcripts expressed in neuronal cells, by analyzingfluorescence-activated cell sorted (FACS) neurons.	DESeq. False discovry rate (FDR) < 0.1.	WBPaper00048988:neuron_expressed
	Genes that showed expression levels higher than the corresponding reference sample (embryonic 24hr reference).	A Mann-Whitney U test with an empirical background model and FDR correction for multiple testing was used to detect expressed transcripts (Benjamini and Hochberg 1995). Genes and TARs with an FDR <= 0.05 were reported as expressed above background. Authors detected differentially expressed transcripts using a method based on linear models. Genes and TARs were called differentially expressed if the FDR was <= 0.05 and the fold change (FC) >= 2.0. To more strictly correct for potential false-positives resulting from multiple sample comparisons, authors divided individual FDR estimates by the number of samplesor sample comparisons, respectively. This resulted in an adjusted FDR of 1.3 * 0.0001 for expression above background and of 7.4 * 0.0001 for differential expression. Authors called genes selectively enriched in a given tissue if they met the following requirements: (1) enriched expression in a given tissue (FDR <= 0.05 and FC >= 2.0), (2) fold change versus reference among the upper 40% of the positive FC range observed for this gene across all tissues, and (3) fold-change entropy among the lower 40% of the distribution observed for all genes.	WBPaper00037950:all-neurons_L1-larva_expressed
adult vs dauer larva	Transcripts that showed differential expression in adult vs dauer lava in N2 animals at 20C.	N.A.	WBPaper00050488:adult_vs_dauer_regulated_N2_20C
	Genes that showed expression levels higher than the corresponding reference sample (embryonic 24hr reference).	A Mann-Whitney U test with an empirical background model and FDR correction for multiple testing was used to detect expressed transcripts (Benjamini and Hochberg 1995). Genes and TARs with an FDR <= 0.05 were reported as expressed above background. Authors detected differentially expressed transcripts using a method based on linear models. Genes and TARs were called differentially expressed if the FDR was <= 0.05 and the fold change (FC) >= 2.0. To more strictly correct for potential false-positives resulting from multiple sample comparisons, authors divided individual FDR estimates by the number of samplesor sample comparisons, respectively. This resulted in an adjusted FDR of 1.3 * 0.0001 for expression above background and of 7.4 * 0.0001 for differential expression. Authors called genes selectively enriched in a given tissue if they met the following requirements: (1) enriched expression in a given tissue (FDR <= 0.05 and FC >= 2.0), (2) fold change versus reference among the upper 40% of the positive FC range observed for this gene across all tissues, and (3) fold-change entropy among the lower 40% of the distribution observed for all genes.	WBPaper00037950:AVE-neuron_L1-larva_expressed
Osmotic stress	Transcripts that showed significantly altered expression with 500 mM salt (NaCl) vs 100 mM salt when food was present	DESeq(version 1.10.1), FDR < 0.05.	WBPaper00050726:OsmoticStress_regulated_Food
Osmotic stress	Transcripts that showed significantly altered expression with 500 mM salt (NaCl) vs 100 mM salt when no food was present	DESeq(version 1.10.1), FDR < 0.05.	WBPaper00050726:OsmoticStress_regulated_NoFood
	Genes that showed expression levels higher than the corresponding reference sample (embryonic 24hr reference).	A Mann-Whitney U test with an empirical background model and FDR correction for multiple testing was used to detect expressed transcripts (Benjamini and Hochberg 1995). Genes and TARs with an FDR <= 0.05 were reported as expressed above background. Authors detected differentially expressed transcripts using a method based on linear models. Genes and TARs were called differentially expressed if the FDR was <= 0.05 and the fold change (FC) >= 2.0. To more strictly correct for potential false-positives resulting from multiple sample comparisons, authors divided individual FDR estimates by the number of samplesor sample comparisons, respectively. This resulted in an adjusted FDR of 1.3 * 0.0001 for expression above background and of 7.4 * 0.0001 for differential expression. Authors called genes selectively enriched in a given tissue if they met the following requirements: (1) enriched expression in a given tissue (FDR <= 0.05 and FC >= 2.0), (2) fold change versus reference among the upper 40% of the positive FC range observed for this gene across all tissues, and (3) fold-change entropy among the lower 40% of the distribution observed for all genes.	WBPaper00037950:bodywall-muscle_L1-larva_expressed
Bacteria infection: Enterococcus faecalis	Genes with increased expression after 24 hours of infection by E.faecalis Fold changes shown are pathogen vs OP50.	For RNA-seq and tiling arrays, log2 fold changes between gene expression values of infected versus uninfected nematodes were calculated. For log2 fold changes > 0.00001 the values > 81.25th percentile were defined as up-regulated and for log2 fold changes < -0.00001 the values < 18.75th percentile were defined as down-regulated.	WBPaper00038438:E.faecalis_24hr_upregulated_TilingArray
	Transcripts that showed significantly increased expression after animals were treated with 50uM Rifampicin and 250uM Allantoin from day 1 to day 3 adult hermaphradite.	DESeq2(v1.14.1), fold change > 2, p-value < 0.05	WBPaper00055354:Rifampicin-Allantoin_upregulated
	Transcripts that showed significantly increased expression after animals were treated with 50uM Rifampicin from day 1 to day 3 adult hermaphradite.	DESeq2(v1.14.1), fold change > 2, p-value < 0.05	WBPaper00055354:Rifampicin_upregulated
	Transcripts that showed significantly increased expression after animals were treated with 100uM Psora and 250uM Allantoin from day 1 to day 3 adult hermaphradite.	DESeq2(v1.14.1), fold change > 2, p-value < 0.05	WBPaper00055354:Psora-Allantoin_upregulated
	Transcripts that showed significantly increased expression after animals were treated with 100uM Rapamycin and 50mM Metformin from day 1 to day 3 adult hermaphradite.	DESeq2(v1.14.1), fold change > 2, p-value < 0.05	WBPaper00055354:Rapamycin-Metformin_upregulated
	Transcripts that showed significantly decreased expression in atfs-1(cmh15) (null allele) animals comparing to in N2 animals at L4 larva stage.	edgeR, fold change > 2, FDR < 0.05	WBPaper00060909:atfs-1(cmh15)_downregulated
	Transcripts that showed significantly increased expression in rrf-3(pk1426) comparing to in N2 at embryo stage.	DESeq2v 1.18.1, fold change > 1.5, adjusted p-value < 0.01.	WBPaper00056169:rrf-3(pk1426)_upregulated_embryo
	Transcripts that showed significantly increased expression after four-day-old young adult worms were placed on NGM plates seeded with OP50 in the presence 5% Agaro-oligosaccharides(AGO) for 24 h, comparing to animals grown in the absence of AGO.	Fold change > 2.	WBPaper00064306:Agaro-oligosaccharides_upregulated
	Transcripts that showed significantly increased expression in sin-3(tm1276) comparing to in N2.	DESeq2, fold change > 2, p-value < 0.01.	WBPaper00061203:sin-3(tm1276)_upregulated
	Transcripts that showed significantly increased expression in aak-1(tm1944);aak-2(ok524) animals comparing to in N2.	DEseq 1.18.0, adjusted p-value < 0.05.	WBPaper00056471:aak-1(tm1944);aak-2(ok524)_upregulated
	Transcripts that showed significantly altered expression after 24 hour exposure to stavudine (d4T) starting at L1 lava stage.	DESeq	WBPaper00053302:stavudine_24h_regulated
	Transcripts that showed significantly decreased expression in sin-3(tm1276) comparing to in N2 at early embryo when there were only 3 -5 eggs in the adult.	DESeq2, fold change > 2, adjusted p-value < 0.01	WBPaper00058598:sin-3(tm1276)_downregulated
Reduced humidity (98% relative humidity).	Genes that were down-regulated after one day exposure to reduced humidity (98% relative humidity) according to microarray analysis.	Multiple hypothesis testing with the Benjamini-Hochberg correction was applied on calculated p-values. A change in the expression level was considered to be significant if the adjusted p-value was less than 0.001.	WBPaper00044578:reduced-humidity_downregulated_microarray
	Genes that showed expression levels higher than the corresponding reference sample (L2 all cell reference).	A Mann-Whitney U test with an empirical background model and FDR correction for multiple testing was used to detect expressed transcripts (Benjamini and Hochberg 1995). Genes and TARs with an FDR <= 0.05 were reported as expressed above background. Authors detected differentially expressed transcripts using a method based on linear models. Genes and TARs were called differentially expressed if the FDR was <= 0.05 and the fold change (FC) >= 2.0. To more strictly correct for potential false-positives resulting from multiple sample comparisons, authors divided individual FDR estimates by the number of samplesor sample comparisons, respectively. This resulted in an adjusted FDR of 1.3 * 0.0001 for expression above background and of 7.4 * 0.0001 for differential expression. Authors called genes selectively enriched in a given tissue if they met the following requirements: (1) enriched expression in a given tissue (FDR <= 0.05 and FC >= 2.0), (2) fold change versus reference among the upper 40% of the positive FC range observed for this gene across all tissues, and (3) fold-change entropy among the lower 40% of the distribution observed for all genes.	WBPaper00037950:coelomocytes_L2-larva_expressed
	Transcripts that showed significantly increased expression in hpk-1(pk1393) comparing to in N2 at adult day 2.	DESeq 2, fold change > 2, FDR < 0.05.	WBPaper00065581:hpk-1(pk1393)_upregulated
	Genes that showed expression levels higher than the corresponding reference sample (L3/L4 all cell reference).	A Mann-Whitney U test with an empirical background model and FDR correction for multiple testing was used to detect expressed transcripts (Benjamini and Hochberg 1995). Genes and TARs with an FDR <= 0.05 were reported as expressed above background. Authors detected differentially expressed transcripts using a method based on linear models. Genes and TARs were called differentially expressed if the FDR was <= 0.05 and the fold change (FC) >= 2.0. To more strictly correct for potential false-positives resulting from multiple sample comparisons, authors divided individual FDR estimates by the number of samplesor sample comparisons, respectively. This resulted in an adjusted FDR of 1.3 * 0.0001 for expression above background and of 7.4 * 0.0001 for differential expression. Authors called genes selectively enriched in a given tissue if they met the following requirements: (1) enriched expression in a given tissue (FDR <= 0.05 and FC >= 2.0), (2) fold change versus reference among the upper 40% of the positive FC range observed for this gene across all tissues, and (3) fold-change entropy among the lower 40% of the distribution observed for all genes.	WBPaper00037950:dopaminergic-neurons_L3-L4-larva_expressed
	Genes that showed expression levels higher than the corresponding reference sample (L2 all cell reference).	A Mann-Whitney U test with an empirical background model and FDR correction for multiple testing was used to detect expressed transcripts (Benjamini and Hochberg 1995). Genes and TARs with an FDR <= 0.05 were reported as expressed above background. Authors detected differentially expressed transcripts using a method based on linear models. Genes and TARs were called differentially expressed if the FDR was <= 0.05 and the fold change (FC) >= 2.0. To more strictly correct for potential false-positives resulting from multiple sample comparisons, authors divided individual FDR estimates by the number of samplesor sample comparisons, respectively. This resulted in an adjusted FDR of 1.3 * 0.0001 for expression above background and of 7.4 * 0.0001 for differential expression. Authors called genes selectively enriched in a given tissue if they met the following requirements: (1) enriched expression in a given tissue (FDR <= 0.05 and FC >= 2.0), (2) fold change versus reference among the upper 40% of the positive FC range observed for this gene across all tissues, and (3) fold-change entropy among the lower 40% of the distribution observed for all genes.	WBPaper00037950:glr-1(+)-neurons_L2-larva_expressed
	Transcripts that showed significantly increased expression in hda-2(ok1479) comparing to in N2 animals.	DESeq2 (version 1.28.1), FDR < 0.01, fold change > 2.	WBPaper00062159:hda-2(ok1479)_upregulated
	Transcripts that showed significantly increased expression in srbc-48(ac23);kyIs262;fer-1(b232ts) comparing to in kyIs262;fer-1(b232ts), 24h after infection with P.aeruginosa.	DESeq2, FDR <0.05, fold change > 2.	WBPaper00059664:srbc-48(ac23)_upregulated
Starvation	Transcripts that showed significantly altered expression by starvation with 100 mM salt (NaCl)	DESeq(version 1.10.1), FDR < 0.05.	WBPaper00050726:starvation_regulated_LowSalt
Bacteria infection: Serratia marcescens	Genes with increased expression after 24 hours of infection by S.marcescens Fold changes shown are pathogen vs OP50.	For RNA-seq and tiling arrays, log2 fold changes between gene expression values of infected versus uninfected nematodes were calculated. For log2 fold changes > 0.00001 the values > 81.25th percentile were defined as up-regulated and for log2 fold changes < -0.00001 the values < 18.75th percentile were defined as down-regulated.	WBPaper00038438:S.marcescens_24hr_upregulated_TilingArray
	Genes expressed in N2.	Expressed transcripts were identified on the basis of a Present call in 3 out of 4 N2 experiments as determined by Affymetrix MAS 5.0.	WBPaper00025141:N2_Expressed_Genes

23 Expression Patterns

• Primary Identifier: Expr4342
• Pattern: In first stage larvae (L1) of both sexes, ceh-22b::VENUS expression was not detected in SGPs(somatic gonadal precursor) at hatching, but became visible midway through the first larval stage (L1). After the SGP divided, the intensity of the reporter began to increase in distal SGP daughters (Z1.a and Z4.p) and began to diminish from proximal SGP daughters (Z1.p and Z4.a). In hermaphrodites, both progeny of the distal SGP daughter retained robust ceh-22b::VENUS expression through L2 or early L3. In males, the distal SGP daughter, which does not divide further, retained strong expression until L3; the expression decreased during L4. The ceh-22b::VENUS reporter was also expressed in the pharynx, intestine, and ventral nerve cord as well as in unidentified neurons in the head and tail. Expression in the pharynx and intestine was sustained throughout larval development into adulthood; expression in the ventral nerve cord was visible until L3.

• Primary Identifier: Expr2009869
• Pattern: Single cell embryonic expression. Only cell types with an expression fraction of greater 0.2 of the maximum expressed fraction are labeled (Full data can be downloaded from http://caltech.wormbase.org/pub/wormbase/datasets-published/packer2019/). The colors represent the broad cell class to which the cell type has been assigned. The size of the point is proportional to the log2 of the numbers of cells in the dataset of that cell type. Interactive visualizations are available as a web app (https://cello.shinyapps.io/celegans/) and can also be installed as an R package (https://github.com/qinzhu/VisCello.celegans).

• Primary Identifier: Expr1030253
• Pattern: Tiling arrays expression graphs

• Primary Identifier: Expr11805
• Remark: Feature : [ceh-22de199::pPD95.27]
• Pattern: The distal enhancer de199 can activate transcription in the pharyngeal muscles, although it exhibits distinct patterns of activity in other cell types. 5Xde199 activated reporter gene expression more broadly than the distal enhancer DE3 in most pharyngeal muscles and in marginal cells, with occasional expression in pharyngeal epithelial cells and in body wall muscles.

• Primary Identifier: Expr11806
• Remark: Feature : [ceh-22de209::pPD95.27]
• Pattern: The distal enhancer de209 can activate transcription in the pharyngeal muscles, although it exhibits distinct patterns of activity in other cell types. 5Xde209 activated reporter expression in a pattern similar to the distal enhancer DE3 in the pharyngeal muscles, with occasional expression in the m6 muscles, marginal cells, and pharyngeal-intestinal valve cells. The parental Dpes-10DlacZ reporter in pPD95.27 was occasionally expressed in posterior gut cells, with < 0.25 h-galactosidase expressing pharyngeal cells transformant.

• Primary Identifier: Expr11811
• Remark: Feature: 'WBsf919537::pPD95.21'
• Pattern: The distal enhancer activated reporter gene expression both inside and outside the pharynx. In larvae and adults, expression was observed in the m3, m4, m5, and m7 pharyngeal muscles as well as pharyngeal marginal cells, epithelial cells, and neurons. Expression was also observed outside the pharynx in the body wall muscles and the ventral nerve cord. Distal enhancer activity initiated in the pharynx at the bean stage of embryogenesis near the time that the endogenous ceh-22 gene is first expressed.

• Primary Identifier: Expr11279
• Remark: Feature : "ceh-22.pe39_pe41"
• Pattern: Both pe39 and pe41 enhanced expression specifically in pharyngeal muscles. Transgenic lines bearing pe39:: pes-10::lacZ and pe41::pes-10::lacZ reporters exhibited robust reporter gene expression in the m3, m4, m5, and m7 pharyngeal muscles, cells that express the endogenous ceh-22 gene. In addition,the pe41::pes-10::lacZ was also expressed in one non muscle cell in the pharynx (provisionally identified as a g1 gland), the pharyngeal-intestinal valve cells, and a pair of neurons outside the pharynx. The onset of pharyngeal-galactosidase expression was as early as the comma stage of embryogenesis, and expression persisted in the pharyngeal muscles through the remainder of embryogenesis and larval development. Both the spatial and temporal specificity of the pe39 enhancer in particular was nearly indistinguishable from that of the full-length proximal enhancer.

• Primary Identifier: Expr1200142
• Pattern: Data from the TransgeneOme project

• Primary Identifier: Expr603
• Remark: Identical staining pattern observed with both antisera (ST::CEH-22 and poly-his::CEH-22 ). Reporter_gene X-gal staining identical to anti-CEH-22 antibodies (Construct contains ~4 kb of ceh-22' -flanking DNA fused to lacZ within the ceh-22 5'-UTR.) This information was extracted from published material (Archana Sharma-Oates, Andrew Mounsey and Ian A. Hope). see Expr748 for western analysis data.
• Pattern: Antibody staining limited to nuclei within pharynx and is detected from beginning of morphogenesis onwards. CEH-22 first detected approx. 330 minutes after fertilization (the lima bean stage) in 11-14 pharyngeal muscle nuclei (probably pharyngeal muscle). At 1.5-fold stage, 14-23 pharyngeal nuclei stain. Cells identified as m3, m4, m5 and m7. In embryos that have completed elongation (pretzel stage), CEH-22 positive nuclei are identified as m3, m4, m5 and m7. Also detected in 6 other pharyngeal nuclei, which are believed to be m1 muscles. After hatching, staining persists in m1, m3, m4, m5 and m7 but absent in m6 and m2.

• Primary Identifier: Expr1795
• Pattern: Expressed in pharynx.

• Primary Identifier: Expr11277
• Remark: Feature : "ceh-22.proximal"
• Pattern: The proximal enhancer activated reporter gene expression specifically in the pharyngeal muscles. In larvae and adults, expression was only observed in the m3, m4, m5, and m7muscles, cells that express the endogenous ceh-22 gene. Proximal enhancer activity initiated as early as the comma stage of embryogenesis later than either the distal enhancer or the endogenous ceh-22 gene.

• Primary Identifier: Expr11802
• Remark: Feature : [ceh-22DE1::pPD95.27]
• Pattern: The distal enhancer DE1 predominantly activated transcription in the m3, m4, m5, and m7 pharyngeal muscles.

• Primary Identifier: Expr11804
• Remark: Feature : [ceh-22DE3::pPD95.27]
• Pattern: The distal enhancer DE3 activated transcription in the m3, m4, m5, and m7 pharyngeal muscles and also activated occasional expression in the pharyngeal-intestinal valve and body wall muscle cells.

• Primary Identifier: Expr11803
• Remark: Feature : [ceh-22DE2::pPD95.27]
• Pattern: The distal enhancer DE2 was highly active in the m1 pharyngeal muscle cells, a specialized syncytial cell forming a thin sheet at the anterior of the pharynx.

• Primary Identifier: Expr11468
• Pattern: ceh-22b::venus was expressed in DTCs until the middle of L4. ceh-22b::venus in DTCs was not detectable in adults. Weaker expression was also observed in the proximal part of the gonad.

• Primary Identifier: Expr11278
• Remark: Feature : "ceh-22.PE1"
• Pattern: PE1, a 140-bp fragment near the 5 end of the proximal enhancer, is the only segment required for enhancer activity in the pharyngeal muscle.

• Primary Identifier: Expr11280
• Remark: Feature : "ceh-22.pe27"
• Pattern: pe27, located near the 5 end of pe1, activated pharyngeal beta-galactosidase expression in 32% of the transformants.

• Primary Identifier: Expr2028109
• Pattern: Single cell embryonic expression. Only cell types with an expression fraction of greater 0.2 of the maximum expressed fraction are labeled (Full data can be downloaded from http://caltech.wormbase.org/pub/wormbase/datasets-published/packer2019/). The colors represent the broad cell class to which the cell type has been assigned. The size of the point is proportional to the log2 of the numbers of cells in the dataset of that cell type. Interactive visualizations are available as a web app (https://cello.shinyapps.io/celegans/) and can also be installed as an R package (https://github.com/qinzhu/VisCello.celegans).

• Primary Identifier: Chronogram878
• Remark: Original chronogram file: chronogram.1920.xml
• Reporter Gene: [F29F11.5:gfp] transcriptional fusion.

• Primary Identifier: Expr1149834
• Pattern: Developmental gene expression time-course. Raw data can be downloaded from ftp://caltech.wormbase.org/pub/wormbase/datasets-published/hashimshony2015

• Primary Identifier: Expr1170044
• Pattern: Time-lapse fluorescence microscopy was performed, including DIC for morphology. Gene expression patterns were summarized in 4 manners: Average over time, Average over time and at different positions along the anterior-posterior (AP) axis, a voxelized representation over time, and on individual cells overlaid from a reference coordinate dataset (https://doi.org/10.1016/j.ydbio.2009.06.014). The analysis was done with a pipeline based on the multi-purpose image analysis software Endrov (https://doi.org/10.1038/nmeth.2478), which further is needed to browse the raw recording data. Thumbnail movies were also generated, using maximum Z projection for the 3D fluorescence channel. Raw recordings available in the Endrov OST-file format are available at https://www.ebi.ac.uk/biostudies/studies/S-BIAD191?query=S-BIAD191

• Primary Identifier: Expr15574

• Primary Identifier: Expr1026044
• Pattern: Developmental gene expression time-course. Raw data can be downloaded from ftp://caltech.wormbase.org/pub/wormbase/datasets-published/levin2012

19 GO Annotation

22 Homologues

1 Locations

• Feature . Primary Identifier: WBGene00000445
• Start: 10671918
• End: 10675769
• Strand: 1

19 Ontology Annotations

0 Regulates Expr Cluster

1 Sequence

• Length: 3852

1 Sequence Ontology Term