WormMine: Gene WBGene00000900

• WormBase Gene ID: WBGene00000900
• Gene Name: daf-4
• Sequence Name: C05D2.1
• Brief Description: daf-4 encodes a transmembrane serine/threonine kinase that is the sole C. elegans ortholog of the type II transforming growth factor-beta (TGF-b) receptors; DAF-4 activity is required for several biological processes, including entry into and exit from the dauer larval stage, normal adult lifespan, body size determination, male tail patterning, egg laying, chemosensory neuron specification, and increased thermotolerance; in regulating dauer larval development, DAF-4 acts in parallel with the insulin signaling pathway and likely partners with the DAF-1 type I TGF-b receptor to receive a signal from the DAF-7 TGF-b ligand; in body size determination and male tail patterning, DAF-4 likely partners with the SMA-6 type I receptor and responds to signals from the DBL-1 ligand; thus daf-4 is utilized by both the Dauer TGF-beta pathway and the TGF-beta Sma/Mab pathway; daf-4 also regulates reproductive aging, via the TGF-beta Sma/Mab pathway, mutants of which show a large reproductive span extension; DAF-4 expression begins in late embryogenesis and continues through larval and adult stages; consistent with its role in many developmental processes, DAF-4 localizes to the plasma membrane of several tissues including head and tail neurons, the pharynx, intestine, ventral nerve cord, tail hypodermis, and body wall muscle.
• Organism: Caenorhabditis elegans
• Automated Description: Enables BMP binding activity. Involved in several processes, including defense response to other organism; regulation of BMP signaling pathway; and regulation of anatomical structure morphogenesis. Located in plasma membrane. Expressed in several structures, including ganglia; intestine; somatic nervous system; tail hypodermis; and vulva. Used to study Marfan syndrome. Human ortholog(s) of this gene implicated in several diseases, including Loeys-Dietz syndrome 2; artery disease (multiple); and gastrointestinal system cancer (multiple). Is an ortholog of human ACVR2B (activin A receptor type 2B).
• Biotype: SO:0001217
• Genetic Position: III :-1.4679 ±0.001027
• Length (nt): 7189

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Synonyms

• Value: WBGene00000900

Genomics

7 Transcripts

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
Transcript:C05D2.1e.1	C05D2.1e.1	2658	III: 5625135-5632322
Transcript:C05D2.1c.1	C05D2.1c.1	2648	III: 5625136-5632307
Transcript:C05D2.1d.1	C05D2.1d.1	1568	III: 5625142-5630098
Transcript:C05D2.1a.1	C05D2.1a.1	2714	III: 5625142-5632322
Transcript:C05D2.1c.2	C05D2.1c.2	2576	III: 5625361-5632323
Transcript:C05D2.1c.3	C05D2.1c.3	1960	III: 5626486-5631843
Transcript:C05D2.1b.1	C05D2.1b.1	1578	III: 5628540-5631843

Other

5 CDSs

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:C05D2.1a	C05D2.1a	2235	III: 5625142-5625282
CDS:C05D2.1b	C05D2.1b	1578	III: 5628540-5628558
CDS:C05D2.1c	C05D2.1c	1779	III: 5626667-5626714
CDS:C05D2.1d	C05D2.1d	1341	III: 5625142-5625282
CDS:C05D2.1e	C05D2.1e	2172	III: 5625142-5625282

12 RNAi Result

• WBRNAi00113200
• WBRNAi00066935
• WBRNAi00068082
• WBRNAi00068208
• WBRNAi00010205
• WBRNAi00005125
• WBRNAi00112970
• WBRNAi00075906
• WBRNAi00039749
• WBRNAi00092532
• WBRNAi00115223
• WBRNAi00115225

120 Allele

• gk964518
• gk175093
• gk175094
• gk175095
• gk175096
• gk175097
• gk175098
• gk175099
• gk175100
• gk175101
• gk175102
• gk175103
• gk175104
• gk964338
• gk964339
• gk964415
• otn13075
• sa183
• sa185
• sa196
• sa220
• sa221
• WBVar02067563
• WBVar02069611
• WBVar01263799
• WBVar01710080
• tm719
• WBVar01541757
• gk524637
• gk878587

1 Chromosome

• WormBase ID: III
• Organism: Caenorhabditis elegans
• Length (nt): 13783801

1 Chromosome Location

• Feature . Primary Identifier: WBGene00000900
• Start: 5625135
• End: 5632323
• Strand: 1

4 Data Sets

• WormBaseAcedbConverter
• GO Annotation data set
• C. elegans genomic annotations (GFF3 Gene)
• Panther orthologue and paralogue predictions

1 Downstream Intergenic Region

• WormBase ID: intergenic_region_chrIII_5632324..5635478
• Length (nt): 3155
• Chromosome Location: III: 5632324-5635478
• Organism: Caenorhabditis elegans

158 Expression Clusters

Regulated By Treatment	Description	Algorithm	Primary Identifier
	Genes with expression altered >= 3-fold in dpy-10(e128) mutants.	Data across the wild type series was analyzed using the Significance analysis of Microarrays (SAM) algorithm (to calculate the False Discovery Rate (FDR)).	WBPaper00035873:dpy-10_regulated
	Transcripts that showed significantly increased expression in L1 neural cells comparing to in adult neural cells.	DESeq2 (v1.18.1) fold change > 2, P-adj<0.05, using BenjaminiHochberg correction.	WBPaper00060811:L1_vs_adult_upregulated_neural
	Transcripts expressed in neuronal cells, by analyzingfluorescence-activated cell sorted (FACS) neurons.	DESeq. False discovry rate (FDR) < 0.1.	WBPaper00048988:neuron_expressed
	Genes that showed expression levels higher than the corresponding reference sample (embryonic 24hr reference).	A Mann-Whitney U test with an empirical background model and FDR correction for multiple testing was used to detect expressed transcripts (Benjamini and Hochberg 1995). Genes and TARs with an FDR <= 0.05 were reported as expressed above background. Authors detected differentially expressed transcripts using a method based on linear models. Genes and TARs were called differentially expressed if the FDR was <= 0.05 and the fold change (FC) >= 2.0. To more strictly correct for potential false-positives resulting from multiple sample comparisons, authors divided individual FDR estimates by the number of samplesor sample comparisons, respectively. This resulted in an adjusted FDR of 1.3 * 0.0001 for expression above background and of 7.4 * 0.0001 for differential expression. Authors called genes selectively enriched in a given tissue if they met the following requirements: (1) enriched expression in a given tissue (FDR <= 0.05 and FC >= 2.0), (2) fold change versus reference among the upper 40% of the positive FC range observed for this gene across all tissues, and (3) fold-change entropy among the lower 40% of the distribution observed for all genes.	WBPaper00037950:all-neurons_L1-larva_expressed
adult vs dauer larva	Transcripts that showed differential expression in adult vs dauer lava in N2 animals at 20C.	N.A.	WBPaper00050488:adult_vs_dauer_regulated_N2_20C
	mRNAs that showed decreased expression in 1 cell mebryo comparing to in oocyte, according to RNAseq analysis.	Gaussian error propagation. As cutoff for the up-regulated genes authors used log2 fold change > 1 and P < 0.05 and as cutoff for the down-regulated genes authors used log2 fold change < -1 and P < 0.05.	WBPaper00045420:fertilization_downregulated_transcript
	Genes that showed expression levels higher than the corresponding reference sample (embryonic 24hr reference).	A Mann-Whitney U test with an empirical background model and FDR correction for multiple testing was used to detect expressed transcripts (Benjamini and Hochberg 1995). Genes and TARs with an FDR <= 0.05 were reported as expressed above background. Authors detected differentially expressed transcripts using a method based on linear models. Genes and TARs were called differentially expressed if the FDR was <= 0.05 and the fold change (FC) >= 2.0. To more strictly correct for potential false-positives resulting from multiple sample comparisons, authors divided individual FDR estimates by the number of samplesor sample comparisons, respectively. This resulted in an adjusted FDR of 1.3 * 0.0001 for expression above background and of 7.4 * 0.0001 for differential expression. Authors called genes selectively enriched in a given tissue if they met the following requirements: (1) enriched expression in a given tissue (FDR <= 0.05 and FC >= 2.0), (2) fold change versus reference among the upper 40% of the positive FC range observed for this gene across all tissues, and (3) fold-change entropy among the lower 40% of the distribution observed for all genes.	WBPaper00037950:bodywall-muscle_L1-larva_expressed
	Transcripts expressed in the epithelial tissues surrounding the pharynx that includes the arcade and intestinal valve (AIV) cells, according to PAT-Seq analysis using Pbath-15-GFP-3XFLAG mRNA tagging.	Cufflinks FPKM value >=1.	WBPaper00050990:arcade_intestinal-valve_expressed
	Transcripts expressed in GABAergic neuron, according to PAT-Seq analysis using Punc-47-GFP-3XFLAG mRNA tagging.	Cufflinks FPKM value >=1.	WBPaper00050990:GABAergic-neuron_expressed
	Transcripts expressed in hypodermis, according to PAT-Seq analysis using Pdpy-7-GFP-3XFLAG mRNA tagging.	Cufflinks FPKM value >=1.	WBPaper00050990:hypodermis_expressed
	Transcripts expressed in intestine, according to PAT-Seq analysis using Pges-1-GFP-3XFLAG mRNA tagging.	Cufflinks FPKM value >=1.	WBPaper00050990:intestine_expressed
	Maternal class (M): genes that are called present in at least one of the three PC6 replicates.	A modified Welch F statistic was used for ANOVA. For each gene, regressed error estimates were substituted for observed error estimates. The substitution is justified by the lack of consistency among the most and least variable genes at each time point. Regressed error estimates were abundance-dependent pooled error estimates that represented a median error estimate from a window of genes of similar abundance to the gene of interest. A randomization test was used to compute the probability Pg of the observed F statistic for gene g under the null hypothesis that developmental time had no effect on expression. P-values were not corrected for multiple testing.	[cgc5767]:expression_class_M
	Transcripts expressed in NMDA neuron, according to PAT-Seq analysis using Pnmr-1-GFP-3XFLAG mRNA tagging.	Cufflinks FPKM value >=1.	WBPaper00050990:NMDA-neuron_expressed
	Strictly maternal class (SM): genes that are the subset of maternal genes that are not also classified as embryonic.	A modified Welch F statistic was used for ANOVA. For each gene, regressed error estimates were substituted for observed error estimates. The substitution is justified by the lack of consistency among the most and least variable genes at each time point. Regressed error estimates were abundance-dependent pooled error estimates that represented a median error estimate from a window of genes of similar abundance to the gene of interest. A randomization test was used to compute the probability Pg of the observed F statistic for gene g under the null hypothesis that developmental time had no effect on expression. P-values were not corrected for multiple testing.	[cgc5767]:expression_class_SM
Bacteria diet: Sphingomonas aquatilis Yellow. Fed for 30 generations.	Transcripts that showed significantly decreased expression after fed by bacteria Sphingomonas aquatilis (Yellow) for 30 generations comparing to animals fed by E. coli OP50.	DESeq2 fold change > 2, p-value < 0.01.	WBPaper00061007:S.aquatilis_downregulated
	Transcripts that showed significantly increased expression in aak-1(tm1944);aak-2(ok524) animals comparing to in N2.	DEseq 1.18.0, adjusted p-value < 0.05.	WBPaper00056471:aak-1(tm1944);aak-2(ok524)_upregulated
Bacteria infection: Staphylococcus aureus MW2. 4 hours of exposure.	Transcripts that showed significantly increased expression after N2 animals had 4 hours of infection by Staphylococcus aureus (MW2).	DEseq 1.18.0, adjusted p-value < 0.05.	WBPaper00056471:S.aureus-4h_upregulated_N2
	Transcripts that showed significantly increased expression in alg-1(gk214), comparing to in N2.	DESeq2, Fold change > 1.5.	WBPaper00051404:alg-1(gk214)_upregulated
	Transcripts that showed significantly increased expression in 10-days post L4 adult hermaphrodite N2 grown at 20C, comparing to in 1-day post L4 adult hermaphrodite N2 animals grown at 20C.	CuffDiff, fold change > 2.	WBPaper00065096:Day10_vs_Day1_upregulated
	Transcripts that showed significantly increased expression in sftb-1(cer6) deletion homozygous comparing to to in N2 animals at L4 larva stage.	DESeq2, fold change > 2	WBPaper00058725:sftb-1(cer6)_downregulated
	Transcripts detected in body muscle nuclei according to a nuclear FACS-based strategy.	Cufflinks	WBPaper00065120:body-muscle-transcriptome
	Genes that showed expression levels higher than the corresponding reference sample (L2 all cell reference).	A Mann-Whitney U test with an empirical background model and FDR correction for multiple testing was used to detect expressed transcripts (Benjamini and Hochberg 1995). Genes and TARs with an FDR <= 0.05 were reported as expressed above background. Authors detected differentially expressed transcripts using a method based on linear models. Genes and TARs were called differentially expressed if the FDR was <= 0.05 and the fold change (FC) >= 2.0. To more strictly correct for potential false-positives resulting from multiple sample comparisons, authors divided individual FDR estimates by the number of samplesor sample comparisons, respectively. This resulted in an adjusted FDR of 1.3 * 0.0001 for expression above background and of 7.4 * 0.0001 for differential expression. Authors called genes selectively enriched in a given tissue if they met the following requirements: (1) enriched expression in a given tissue (FDR <= 0.05 and FC >= 2.0), (2) fold change versus reference among the upper 40% of the positive FC range observed for this gene across all tissues, and (3) fold-change entropy among the lower 40% of the distribution observed for all genes.	WBPaper00037950:A-class-motor-neurons_L2-larva_expressed
Temprature shift to 28C for 48 hours.	Transcripts that showed significantly decreased expression after animals were exposed to 28C temperature for 48 hours.	Differentially expressed genes wereidentified using DESeq (v.1.18.0) by normalizing readsbased on the negative binomial distribution method andcomparing each HS timepoint to the 0-h control.	WBPaper00061341:28C_48h_downregulated
	Genes that showed expression levels higher than the corresponding reference sample (L2 all cell reference).	A Mann-Whitney U test with an empirical background model and FDR correction for multiple testing was used to detect expressed transcripts (Benjamini and Hochberg 1995). Genes and TARs with an FDR <= 0.05 were reported as expressed above background. Authors detected differentially expressed transcripts using a method based on linear models. Genes and TARs were called differentially expressed if the FDR was <= 0.05 and the fold change (FC) >= 2.0. To more strictly correct for potential false-positives resulting from multiple sample comparisons, authors divided individual FDR estimates by the number of samplesor sample comparisons, respectively. This resulted in an adjusted FDR of 1.3 * 0.0001 for expression above background and of 7.4 * 0.0001 for differential expression. Authors called genes selectively enriched in a given tissue if they met the following requirements: (1) enriched expression in a given tissue (FDR <= 0.05 and FC >= 2.0), (2) fold change versus reference among the upper 40% of the positive FC range observed for this gene across all tissues, and (3) fold-change entropy among the lower 40% of the distribution observed for all genes.	WBPaper00037950:all-neurons_L2-larva_expressed
	Transcripts that showed significantly increased expression in daf-2(e1370) comparing to in N2.	Differential gene expression analysis was performed using the quasi-likeli-hood framework in edgeR package v. 3.20.1 in R v. 3.4.1.	WBPaper00053810:daf-2(e1370)_upregulated
	Transcripts that showed significantly increased expression in isp-1(qm150) comparing to in N2.	Differential gene expression analysis was performed using the quasi-likeli-hood framework in edgeR package v. 3.20.1 in R v. 3.4.1.	WBPaper00053810:isp-1(qm150)_upregulated
	Transcripts that showed significantly increased expression in nuo-6(qm200) comparing to in N2.	Differential gene expression analysis was performed using the quasi-likeli-hood framework in edgeR package v. 3.20.1 in R v. 3.4.1.	WBPaper00053810:nuo-6(qm200)_upregulated
	Genes that showed expression levels higher than the corresponding reference sample (L2 all cell reference).	A Mann-Whitney U test with an empirical background model and FDR correction for multiple testing was used to detect expressed transcripts (Benjamini and Hochberg 1995). Genes and TARs with an FDR <= 0.05 were reported as expressed above background. Authors detected differentially expressed transcripts using a method based on linear models. Genes and TARs were called differentially expressed if the FDR was <= 0.05 and the fold change (FC) >= 2.0. To more strictly correct for potential false-positives resulting from multiple sample comparisons, authors divided individual FDR estimates by the number of samplesor sample comparisons, respectively. This resulted in an adjusted FDR of 1.3 * 0.0001 for expression above background and of 7.4 * 0.0001 for differential expression. Authors called genes selectively enriched in a given tissue if they met the following requirements: (1) enriched expression in a given tissue (FDR <= 0.05 and FC >= 2.0), (2) fold change versus reference among the upper 40% of the positive FC range observed for this gene across all tissues, and (3) fold-change entropy among the lower 40% of the distribution observed for all genes.	WBPaper00037950:coelomocytes_L2-larva_expressed
	Genes that showed expression levels higher than the corresponding reference sample (L3/L4 all cell reference).	A Mann-Whitney U test with an empirical background model and FDR correction for multiple testing was used to detect expressed transcripts (Benjamini and Hochberg 1995). Genes and TARs with an FDR <= 0.05 were reported as expressed above background. Authors detected differentially expressed transcripts using a method based on linear models. Genes and TARs were called differentially expressed if the FDR was <= 0.05 and the fold change (FC) >= 2.0. To more strictly correct for potential false-positives resulting from multiple sample comparisons, authors divided individual FDR estimates by the number of samplesor sample comparisons, respectively. This resulted in an adjusted FDR of 1.3 * 0.0001 for expression above background and of 7.4 * 0.0001 for differential expression. Authors called genes selectively enriched in a given tissue if they met the following requirements: (1) enriched expression in a given tissue (FDR <= 0.05 and FC >= 2.0), (2) fold change versus reference among the upper 40% of the positive FC range observed for this gene across all tissues, and (3) fold-change entropy among the lower 40% of the distribution observed for all genes.	WBPaper00037950:dopaminergic-neurons_L3-L4-larva_expressed
	Genes that showed expression levels higher than the corresponding reference sample (L2 all cell reference).	A Mann-Whitney U test with an empirical background model and FDR correction for multiple testing was used to detect expressed transcripts (Benjamini and Hochberg 1995). Genes and TARs with an FDR <= 0.05 were reported as expressed above background. Authors detected differentially expressed transcripts using a method based on linear models. Genes and TARs were called differentially expressed if the FDR was <= 0.05 and the fold change (FC) >= 2.0. To more strictly correct for potential false-positives resulting from multiple sample comparisons, authors divided individual FDR estimates by the number of samplesor sample comparisons, respectively. This resulted in an adjusted FDR of 1.3 * 0.0001 for expression above background and of 7.4 * 0.0001 for differential expression. Authors called genes selectively enriched in a given tissue if they met the following requirements: (1) enriched expression in a given tissue (FDR <= 0.05 and FC >= 2.0), (2) fold change versus reference among the upper 40% of the positive FC range observed for this gene across all tissues, and (3) fold-change entropy among the lower 40% of the distribution observed for all genes.	WBPaper00037950:excretory-cell_L2-larva_expressed

12 Expression Patterns

• Primary Identifier: Expr1030564
• Pattern: Tiling arrays expression graphs

• Primary Identifier: Expr15986
• Pattern: We found that DAF-4::GFP::3xFLAG is expressed in multiple tissues, including the pharynx, hypodermis, intestine, and developing vulva. Within each cell, DAF-4::GFP::3xFLAG is primarily localized to the cell surface. We also detected some GFP signal in intracellular vesicles in hypodermal cells. Notably, in both the intestine and the vulva, 2 types of polarized epithelial cells, DAF-4::GFP::3xFLAG is localized to the basolateral membrane but absent on the apical membrane. The basolateral localization of DAF-4::GFP::3xFLAG in the intestinal cells has also been previously reported by Gleason and colleagues using a functional, intestinally expressed DAF-4::GFP.

• Primary Identifier: Expr948
• Remark: GFP expression matched the expression pattern of a daf-4::gfp gene fusion (Patterson et al., 1997), suggesting that daf-4 regulatory sequences conferring tissue-specificity are upstream of the transcription start site.
• Pattern: GFP was expressed in the pharynx, intestine, hypodermis and body wall muscles, in L1 through adult stages. In the head, GFP was seen in neurons of the lateral, vesicular and retrovesicular ganglia. Ventral cord neurons also were visible, as was the PVT neuron, but only two phasmid neurons were detected in the tail. Whereas the nervous system is the primary site of gfp expression mediated by the daf-1 promoter, the daf-4 promoter directs expression more broadly, consistent with its other functions. In L1 larvae, when the dauer/nondauer decision is made, daf-4 promoter is active in neurons in the head, as well as in the ventral cord and tail. The promoters continues to express GFP in dauer larvae from starved plates.

• Primary Identifier: Expr1456
• Pattern: Almost every DAF-4/GFP transgenic animal shows strong GFP fluorescence in many, but not all, head neurons, the ventral nerve cord (both cell bodies and processes,), intestinal cells, and tail neurons. DAF-4/GFP is expressed in the pharynx, and weakly in the ventral nerve cord cell bodies. DAF-4/GFP is expressed rarely in main body hypodermis, but is expressed in tail hypodermis. This apparent difference in the tail versus the main body may be attributable to effects of the anatomy on scoring. DAF-4/GFP is not localized to the membrane surrounding the intestinal lumen, nor is it expressed in the distal tip cell or its precursors. Expression of DAF-4/GFP is similar in larval stages and adults. DAF-4/GFP is first detectable at late embryogenesis when the embryo resembles an L1 larva. DAF-4/GFP is not expressed in early embryos.
• Subcellular Localization: DAF-4/GFP is localized to membranes.

• Primary Identifier: Expr1021709
• Pattern: Developmental gene expression time-course. Raw data can be downloaded from ftp://caltech.wormbase.org/pub/wormbase/datasets-published/levin2012

• Primary Identifier: Expr11050
• Pattern: DAF-4::YFP fusion protein localizes to the plasma membranes of hyp8-11 during tail tip morphogenesis.

• Primary Identifier: Expr2010779
• Pattern: Single cell embryonic expression. Only cell types with an expression fraction of greater 0.2 of the maximum expressed fraction are labeled (Full data can be downloaded from http://caltech.wormbase.org/pub/wormbase/datasets-published/packer2019/). The colors represent the broad cell class to which the cell type has been assigned. The size of the point is proportional to the log2 of the numbers of cells in the dataset of that cell type. Interactive visualizations are available as a web app (https://cello.shinyapps.io/celegans/) and can also be installed as an R package (https://github.com/qinzhu/VisCello.celegans).

• Primary Identifier: Expr2029016
• Pattern: Single cell embryonic expression. Only cell types with an expression fraction of greater 0.2 of the maximum expressed fraction are labeled (Full data can be downloaded from http://caltech.wormbase.org/pub/wormbase/datasets-published/packer2019/). The colors represent the broad cell class to which the cell type has been assigned. The size of the point is proportional to the log2 of the numbers of cells in the dataset of that cell type. Interactive visualizations are available as a web app (https://cello.shinyapps.io/celegans/) and can also be installed as an R package (https://github.com/qinzhu/VisCello.celegans).

• Primary Identifier: Expr1143840
• Pattern: Developmental gene expression time-course. Raw data can be downloaded from ftp://caltech.wormbase.org/pub/wormbase/datasets-published/hashimshony2015

• Primary Identifier: Expr14525
• Subcellular Localization: the wild-type TGFβ type II receptors are located along the basolateral membranes and in a net-like distribution with little to no localization along the apical cell boundary.

• Primary Identifier: Expr3040
• Pattern: Northern hybridization signals from daf-4 2.0 kb and 2.9 kb RNAs were quantified using a phosphorimager, and then evaluated by the ratio of 2.0 to 2.9 kb signal for each sample. The 2.0 kb transcript was slightly more abundant than the 2.9 kb during all stages grown in abundant food, with a ratio between 1.2 and 1.9 in L2-L4 larvae and adults. In cultures where food was scarce, and animals were crowded, developing larvae entered and remained in dauer diapause, and the ratio of 2.0 to 2.9 kb transcript signals increased to 5.0. Dauer larvae transferred to fresh medium with plentiful food resumed growth through post-dauer stages PD1 and PD2, and the 2.0 to 2.9 kb ratio decreased correspondingly to levels in well fed populations.

• Primary Identifier: Expr3041
• Pattern: In favorable growth conditions, copy number of the 2.0 kb product decreased slightly as animals developed through larval stages, but returned to L1 levels in the adult. Copy number of the 2.9 kb product also demonstrated minor deviations from L1 levels. In dauer larvae, there is a 2-fold increase in full-length daf-4, and a 13-fold increase in the 2.0 kb transcript. Post-dauer animals (PD1, PD2) that recovered from diapause in plentiful food show a reduction in the amount of both transcripts to near-L1 levels.

61 GO Annotation

12 Homologues

1 Locations

• Feature . Primary Identifier: WBGene00000900
• Start: 5625135
• End: 5632323
• Strand: 1

61 Ontology Annotations

0 Regulates Expr Cluster

1 Sequence

• Length: 7189

1 Sequence Ontology Term