WormMine: Gene WBGene00000908

• WormBase Gene ID: WBGene00000908
• Gene Name: daf-12
• Sequence Name: F11A1.3
• Brief Description: daf-12 encodes a member of the steroid hormone receptor superfamily that is homologous to human VITAMIN D RECEPTOR (VDR; OMIM:601769, mutated in vitamin D-resistant rickets); daf-12 affects dauer formation downstream of the TGF- and insulin signaling pathways, and affects gonad-dependent adult longevity together with DAF-16, chemosensory signal transduction, and distal tip cell migration and hypodermal and intestinal cell lineages during the L3 larval stage; DAF-12 is expressed in the nucleus and in most cells; daf-12 expression in lateral seam cells is negatively regulated by the let-7 miRNA; daf-12 was identified as a key factor mediating the increased resistance against heat-stress induced by carnitine-supplementation in glucose-treated TK22 nematodes.
• Organism: Caenorhabditis elegans
• Automated Description: Enables nuclear receptor activity; sequence-specific DNA binding activity; and steroid hormone binding activity. Involved in several processes, including determination of adult lifespan; heat acclimation; and positive regulation of dauer larval development. Located in nucleus. Expressed in several structures, including gonad; neurons; pharyngeal muscle cell; somatic gonad precursor; and vulva. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); autosomal recessive limb-girdle muscular dystrophy type 2B; and carcinoma (multiple). Is an ortholog of human VDR (vitamin D receptor).
• Biotype: SO:0001217
• Genetic Position: X :2.367±
• Length (nt): 30968

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Synonyms

• Value: WBGene00000908

Genomics

10 Transcripts

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
Transcript:F11A1.3e.3	F11A1.3e.3	3817	X: 10635908-10666875
Transcript:F11A1.3g.1	F11A1.3g.1	2245	X: 10644409-10665481
Transcript:F11A1.3e.1	F11A1.3e.1	3636	X: 10644409-10666872
Transcript:F11A1.3a.1	F11A1.3a.1	3656	X: 10644440-10666875
Transcript:F11A1.3e.4	F11A1.3e.4	3592	X: 10647057-10666875
Transcript:F11A1.3e.2	F11A1.3e.2	3859	X: 10659339-10666875
Transcript:F11A1.3f.1	F11A1.3f.1	2253	X: 10659551-10665481
Transcript:F11A1.3d.1	F11A1.3d.1	2160	X: 10659692-10665481
Transcript:F11A1.3b.1	F11A1.3b.1	2085	X: 10660802-10665481
Transcript:F11A1.3c.1	F11A1.3c.1	2194	X: 10664406-10666871

Other

7 CDSs

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:F11A1.3g	F11A1.3g	2214	X: 10644440-10644550
CDS:F11A1.3a	F11A1.3a	2262	X: 10644440-10644550
CDS:F11A1.3f	F11A1.3f	2112	X: 10659692-10659760
CDS:F11A1.3d	F11A1.3d	2160	X: 10659692-10659760
CDS:F11A1.3e	F11A1.3e	2037	X: 10660802-10660868
CDS:F11A1.3b	F11A1.3b	2085	X: 10660802-10660868
CDS:F11A1.3c	F11A1.3c	804	X: 10664406-10664585

37 RNAi Result

• WBRNAi00067921
• WBRNAi00044333
• WBRNAi00013094
• WBRNAi00013096
• WBRNAi00025073
• WBRNAi00025074
• WBRNAi00084742
• WBRNAi00087794
• WBRNAi00084747
• WBRNAi00084745
• WBRNAi00084752
• WBRNAi00084753
• WBRNAi00106468
• WBRNAi00027886
• WBRNAi00027272
• WBRNAi00106472
• WBRNAi00086614
• WBRNAi00106476
• WBRNAi00077530
• WBRNAi00106477
• WBRNAi00106481
• WBRNAi00101960
• WBRNAi00102715
• WBRNAi00086607
• WBRNAi00086606
• WBRNAi00077516
• WBRNAi00092846
• WBRNAi00023188
• WBRNAi00023409
• WBRNAi00023700

564 Allele

• gk964260
• gk962707
• WBVar01928195
• WBVar01928196
• WBVar01928197
• WBVar01928198
• WBVar01928199
• WBVar01928200
• WBVar01928201
• WBVar01928202
• WBVar01928203
• WBVar01928204
• WBVar01928205
• rh61
• rh61rh412
• rh61rh411
• rh62rh157
• rh62
• rh84
• rh157
• rh193
• rh257
• rh258
• rh274
• rh273
• rh284
• rh286
• rh285
• rh411
• rh412

1 Chromosome

• WormBase ID: X
• Organism: Caenorhabditis elegans
• Length (nt): 17718942

1 Chromosome Location

• Feature . Primary Identifier: WBGene00000908
• Start: 10635908
• End: 10666875
• Strand: 1

4 Data Sets

• WormBaseAcedbConverter
• GO Annotation data set
• C. elegans genomic annotations (GFF3 Gene)
• Panther orthologue and paralogue predictions

1 Downstream Intergenic Region

• WormBase ID: intergenic_region_chrX_10666876..10667011
• Length (nt): 136
• Chromosome Location: X: 10666876-10667011
• Organism: Caenorhabditis elegans

160 Expression Clusters

Regulated By Treatment	Description	Algorithm	Primary Identifier
	Transcripts that showed significantly increased expression in L1 neural cells comparing to in adult neural cells.	DESeq2 (v1.18.1) fold change > 2, P-adj<0.05, using BenjaminiHochberg correction.	WBPaper00060811:L1_vs_adult_upregulated_neural
	Transcripts of coding genes that showed significantly decreased expression in muscle.	DESeq2 (version 1.24.0). Transcripts with a false-discovery rate adjusted p-value less than 0.05 were considered significantly differentially expressed.	WBPaper00062325:muscle_depleted_coding-RNA
	Transcripts expressed in neuronal cells, by analyzingfluorescence-activated cell sorted (FACS) neurons.	DESeq. False discovry rate (FDR) < 0.1.	WBPaper00048988:neuron_expressed
adult vs dauer larva	Transcripts that showed differential expression in adult vs dauer lava in N2 animals at 20C.	N.A.	WBPaper00050488:adult_vs_dauer_regulated_N2_20C
Osmotic stress	Transcripts that showed significantly altered expression with 500 mM salt (NaCl) vs 100 mM salt when food was present	DESeq(version 1.10.1), FDR < 0.05.	WBPaper00050726:OsmoticStress_regulated_Food
	Transcripts that showed significantly increased expression glp-1(e2141); TU3401 animals comparing to in TU3401 animals.	Fold change > 2, FDR < 0.01.	WBPaper00065993:glp-1(e2141)_upregulated
	Coexpression clique No. 60, 176662_at-Y53F4B.16, on the genome-wide coexpression clique map for the nematode GPL200 platform.	All available microarray datasets for the GPL200 platform (Affymetrix C. elegans Genome Array) were obtained from the GEO repository. This included 2243 individual microarray experiments. These were normalized against each other with the software RMAexpress (Bolstad, 2014). Based on these normalized values, Pearsons correlation coefficients were obtained for each probe-probe pair of the 22,620 probes represented on this array type. The resulting list of correlation coefficients was then ranked to generate the ranked coexpression database with information on each probe represented on the GPL200 platform.	WBPaper00061527:176662_at-Y53F4B.16
	Transcripts expressed in body muscle, according to PAT-Seq analysis using Pmyo-3-GFP-3XFLAG mRNA tagging.	Cufflinks FPKM value >=1.	WBPaper00050990:body-muscle_expressed
	Transcripts expressed in GABAergic neuron, according to PAT-Seq analysis using Punc-47-GFP-3XFLAG mRNA tagging.	Cufflinks FPKM value >=1.	WBPaper00050990:GABAergic-neuron_expressed
	Transcripts expressed in hypodermis, according to PAT-Seq analysis using Pdpy-7-GFP-3XFLAG mRNA tagging.	Cufflinks FPKM value >=1.	WBPaper00050990:hypodermis_expressed
	Transcripts that showed significantly decreased expression in atfs-1(cmh15) (null allele) animals comparing to in N2 animals at L4 larva stage.	edgeR, fold change > 2, FDR < 0.05	WBPaper00060909:atfs-1(cmh15)_downregulated
	Transcripts expressed in intestine, according to PAT-Seq analysis using Pges-1-GFP-3XFLAG mRNA tagging.	Cufflinks FPKM value >=1.	WBPaper00050990:intestine_expressed
	Transcripts expressed in NMDA neuron, according to PAT-Seq analysis using Pnmr-1-GFP-3XFLAG mRNA tagging.	Cufflinks FPKM value >=1.	WBPaper00050990:NMDA-neuron_expressed
	Transcripts expressed in pharynx, according to PAT-Seq analysis using Pmyo-2-GFP-3XFLAG mRNA tagging.	Cufflinks FPKM value >=1.	WBPaper00050990:pharynx_expressed
	Genes with expression level regulated by genotype (N2 vs CB4856) and age at old adults stage (214 hours at 24 centigrade).	For model 2, authors used 100 permutations to estimate the FDR threshold. Per permutation, genotypes and ages were independently randomly distributed, keeping the among-gene structure intact. Then for each spot (23,232) on the array, model 2 was tested. The obtained P-values were used to estimate a threshold for each of the explanatory factors. Authors also used a genome-wide threshold of -log10 P-value = 2, which resembles an FDR of 0.072 and 0.060 for marker and the interaction age-marker for the developing worms and FDR of 0.050 and 0.065 for marker and age-marker for the aging worms. For the physiological age effect, authors used a log10 P-value = 8 in developing worms (0.012 FDR) and -log10 P-value = 6 (0.032 FDR).	WBPaper00040858:eQTL_age_regulated_aging
	Genes with expression level regulated by genotype (N2 vs CB4856) and age at L3 larva and Late reproduction stage (96 hours at 24 centigrade).	For model 2, authors used 100 permutations to estimate the FDR threshold. Per permutation, genotypes and ages were independently randomly distributed, keeping the among-gene structure intact. Then for each spot (23,232) on the array, model 2 was tested. The obtained P-values were used to estimate a threshold for each of the explanatory factors. Authors also used a genome-wide threshold of -log10 P-value = 2, which resembles an FDR of 0.072 and 0.060 for marker and the interaction age-marker for the developing worms and FDR of 0.050 and 0.065 for marker and age-marker for the aging worms. For the physiological age effect, authors used a log10 P-value = 8 in developing worms (0.012 FDR) and -log10 P-value = 6 (0.032 FDR).	WBPaper00040858:eQTL_age_regulated_developing
	Genes with expression level regulated by genotype (N2 vs CB4856) at old adults stage (214 hours at 24 centigrade).	For model 2, authors used 100 permutations to estimate the FDR threshold. Per permutation, genotypes and ages were independently randomly distributed, keeping the among-gene structure intact. Then for each spot (23,232) on the array, model 2 was tested. The obtained P-values were used to estimate a threshold for each of the explanatory factors. Authors also used a genome-wide threshold of -log10 P-value = 2, which resembles an FDR of 0.072 and 0.060 for marker and the interaction age-marker for the developing worms and FDR of 0.050 and 0.065 for marker and age-marker for the aging worms. For the physiological age effect, authors used a log10 P-value = 8 in developing worms (0.012 FDR) and -log10 P-value = 6 (0.032 FDR).	WBPaper00040858:eQTL_regulated_aging
	Genes with expression level regulated by genotype (N2 vs CB4856) at Late reproduction stage (96 hours at 24 centigrade).	Authors permuted transcript values and used a genome-wide threshold of log10 P-value = 2, which resembles a false discovery rate (FDR) of 0.0118.	WBPaper00040858:eQTL_regulated_reproductive
	Transcripts that showed significantly increased expression in sin-3(tm1276) comparing to in N2.	DESeq2, fold change > 2, p-value < 0.01.	WBPaper00061203:sin-3(tm1276)_upregulated
	Transcripts that showed significantly increased expression in aak-1(tm1944);aak-2(ok524) animals comparing to in N2.	DEseq 1.18.0, adjusted p-value < 0.05.	WBPaper00056471:aak-1(tm1944);aak-2(ok524)_upregulated
	Transcripts that showed significantly increased expression in alg-1(gk214), comparing to in N2.	DESeq2, Fold change > 1.5.	WBPaper00051404:alg-1(gk214)_upregulated
	Genes that showed significantly increased expression in daf-2(e1370) comparing to in N2.	To identify DEGs, Students t test and the log2 median ratio test were performed to compute t values and median ratios for all the annotated genes. The adjusted P values from each test were computed using an empirical distribution of the null hypothesis, which was obtained from random permutations of the samples. Finally, the adjusted P values from the individual tests were combined to compute the overall P values using Stouffers method , and genes with overall P < 0.05 and fold change > 1.5 were selected as DEGs.	WBPaper00047131:daf-2(e1370)_upregulated_N2-background
	Transcripts that showed significantly decreased expression in N2 animals exposed to 0.1mM Paraquat from hatching to reaching adult stage.	DESeq2 version 1.22.2, p < 0.05	WBPaper00064716:paraquat_downregulated
	Transcripts that showed significantly altered expression after 24 hour exposure to stavudine (d4T) starting at L1 lava stage.	DESeq	WBPaper00053302:stavudine_24h_regulated
	Transcripts that showed significantly decreased expression in sin-3(tm1276) comparing to in N2 at early embryo when there were only 3 -5 eggs in the adult.	DESeq2, fold change > 2, adjusted p-value < 0.01	WBPaper00058598:sin-3(tm1276)_downregulated
	Transcripts depleted in purified oocyte P bodies comparing to in whole oocytes.	DESeq2, FDR < 0.05, fold change > 2.	WBPaper00065975:P-body_vs_oocyte_depleted
	Transcripts that showed differential expression between 24 and 26 hours post hatching L2d and dauer committed larvae of daf-9(dh6), triggered by the dafachronic acid (DA) growth hormone. Cluster 3 genes increased expression transiently at 26 hour post hatching.	Benjamini Hochberg corrected q-value < 0.01.	WBPaper00053388:dauer_regulated_Cluster3
	Transcripts depleted in purified oocyte P bodies comparing to in the whole animal.	DESeq2, FDR < 0.05, fold change > 2.	WBPaper00065975:P-body_vs_WholeAnimal_depleted
	Transcripts that showed significantly increased expression in sftb-1(cer6) deletion homozygous comparing to to in N2 animals at L4 larva stage.	DESeq2, fold change > 2	WBPaper00058725:sftb-1(cer6)_downregulated
Reduced humidity (98% relative humidity).	Genes that were down-regulated after one day exposure to reduced humidity (98% relative humidity) according to microarray analysis.	Multiple hypothesis testing with the Benjamini-Hochberg correction was applied on calculated p-values. A change in the expression level was considered to be significant if the adjusted p-value was less than 0.001.	WBPaper00044578:reduced-humidity_downregulated_microarray

10 Expression Patterns

• Primary Identifier: Expr1030570
• Pattern: Tiling arrays expression graphs

• Primary Identifier: Expr2590
• Remark: Reporter gene fusion type not specified.
• Pattern: Animals expressing the daf-12::GFP construct display the highest levels of GFP throughout the procorpal region in the pharyngeal muscle (pm3) cells. GFP is also expressed throughout the metacorpus (pm4 cells) as well as the isthmus (pm5 cells) and portions of the terminal bulb (pm6). These transgenic animals appear to express GFP throughout the muscles of the pharynx and the pattern of expression is similar to uorescently labeled phalloidin which stains muscles in C. elegans. GFP orescence is detected in each developmental stage including dauer larvae. L2d animals, grown on pheromone plates, express GFP in the same cells, though it appeared brighter than animals of the other stages.

• Primary Identifier: Expr1047
• Remark: Lineage expression: M lineage. The transgene rescued the Daf-d phenotype of rh61rh411 animals, suggesting that daf-12::GFP is functional. Several integrated transgenic lines were made and all gave a similar pattern of expression.
• Pattern: DAF-12::GFP was expressed widely in most cells including tissues modified for dauer formation or by stage. It was expressed in phenotypically affected target tissues (e.g., epidermis, vulva, somatic gonad, intestine, pharynx, sex myoblasts), as well as other tissues with no known phenotype (e.g., nervous system, body wall muscle). Expression was seen from embryo to adult, but was most elevated and widespread during L2. Epidermis: In seam cells and hypodermis, DAF-12::GFP expression was first seen at the 3-fold stage of embryogenesis, increased by late L1, peaked during L2, diminished by late L3, and was low or off in L4 and young adults. Expression was also seen in the ventral epidermal L1 P ectoblasts, L2 vulval precursors, and their L3 descendants. Expression continued during L4 vulval morphogenesis and persisted occasionally in the mature adult vulva at reduced levels. Somatic Gonad: Faint expression was seen as early as L1 in Z1 and Z4 somatic gonadal precursors. By L2, their descendants, the somatic gonadoblasts, including the migratory distal-tip cell, strongly expressed DAF-12::GFP. Expression continued in somatic gonadoblast descendants and distal-tip cells in L3 and early L4. In the adult, expression was robust in the mature spermatheca and uterus. Intestine: Expression in intestinal nuclei was diffuse during the larval stages, but became somewhat stronger in the adult. Nervous System: Only a handful of head and tail neurons expressed GFP early in L1. By mid-L2, DAF-12::GFP was expressed strongly throughout the nervous system, including the ventral cord and peripheral neuroblasts. Expression continued in many neurons in the adult, albeit at reduced levels. Musculature: Expression in body wall muscles became visible by late L1 and L2. Expression continued at later larval stages and in the adult at reduced levels. Expression in pharyngeal muscle was strong by L2 and downregulated by adult. DAF-12::GFP was also expressed in the L1 M-mesoblast, and its derivatives, including post-embryonic body wall muscles, sex myoblasts and their descendants. Dauer formation: DAF-12::GFP was downregulated in dauer larvae in all tissues, but perdured in the somatic gonad and occasional neurons. Upon recovery from dauer diapause, DAF-12::GFP was expressed weakly in most tissues.
• Subcellular Localization: DAF-12::GFP localized primarily to the nucleus, except during mitosis, when expression became diffuse.

• Primary Identifier: Expr1200225
• Pattern: Data from the TransgeneOme project

• Primary Identifier: Expr2010762
• Pattern: Single cell embryonic expression. Only cell types with an expression fraction of greater 0.2 of the maximum expressed fraction are labeled (Full data can be downloaded from http://caltech.wormbase.org/pub/wormbase/datasets-published/packer2019/). The colors represent the broad cell class to which the cell type has been assigned. The size of the point is proportional to the log2 of the numbers of cells in the dataset of that cell type. Interactive visualizations are available as a web app (https://cello.shinyapps.io/celegans/) and can also be installed as an R package (https://github.com/qinzhu/VisCello.celegans).

• Primary Identifier: Expr3460
• Pattern: For N2, DAF-12 was found predominantly at L2 and L3 stages, decreasing in L4.

• Primary Identifier: Expr1113
• Pattern: mRNA is detected at all stages of development. The relative levels of mRNA were quantitated, the highest levels were observed in egg and L2d samples. An approximately 2-fold increase in daf-12 expression is observed in L2d pre-dauer compared to the level detected in eggs. L1 and L2 levels are lower than egg levels. Although very low, daf-12 mRNA is detected in the post-dauer-1 (PD1) stage, in which animals recovering from the dauer stage resume normal pharyngeal pumping and resume growth. The daf-12 mRNA is more easily detected in samples of post dauer-2 (PD2) larvae that are morphologically similar to an L4 animal [1]. The daf-12 mRNA is detected at approximately equivalent levels for L3, L4 and adult stages, although they are lower than the early developmental stages.

• Primary Identifier: Expr2028999
• Pattern: Single cell embryonic expression. Only cell types with an expression fraction of greater 0.2 of the maximum expressed fraction are labeled (Full data can be downloaded from http://caltech.wormbase.org/pub/wormbase/datasets-published/packer2019/). The colors represent the broad cell class to which the cell type has been assigned. The size of the point is proportional to the log2 of the numbers of cells in the dataset of that cell type. Interactive visualizations are available as a web app (https://cello.shinyapps.io/celegans/) and can also be installed as an R package (https://github.com/qinzhu/VisCello.celegans).

• Primary Identifier: Expr1148270
• Pattern: Developmental gene expression time-course. Raw data can be downloaded from ftp://caltech.wormbase.org/pub/wormbase/datasets-published/hashimshony2015

• Primary Identifier: Expr1010206
• Pattern: Developmental gene expression time-course. Raw data can be downloaded from ftp://caltech.wormbase.org/pub/wormbase/datasets-published/levin2012

39 GO Annotation

13 Homologues

1 Locations

• Feature . Primary Identifier: WBGene00000908
• Start: 10635908
• End: 10666875
• Strand: 1

39 Ontology Annotations

8 Regulates Expr Cluster

Regulated By Treatment	Description	Algorithm	Primary Identifier
	Genes regulated by DAF-12, according to whole transcriptome profiling to compare genome-wide regulatory influences of DPY-21 and SET-4 to those of the key transcription factors controlling dauer arrest in eak-7;akt-1 animals, DAF-16 and DAF-12.	Authors identified genes differentially expressed between wild-type and eak-7;akt-1 double mutant animals [fold change >= 1.5 and false discovery rate (FDR) < 0.05]. Authors then compared the transcriptomes of eak-7;akt-1 double mutants to those of eak-7;akt-1 animals harboring mutations in dpy-21, set-4, daf-16, or daf-12, and identified genes that are differentially expressed in the opposite direction as in wild-type relative to eak-7;akt-1. Annotated gene expression data output from CuffDiff v2.2.1 was read into R version 3.2.1 for six comparisons: eak-7;akt-1 compared to (1) wild-type, (2) daf-16(mu86);eak-7;akt-1, (3) daf-12;eak-7;akt-1, (4) set-4(n4600);eak-7;akt-1, (5) set-4(dp268);eak-7;akt-1, and (6) dpy-21;eak-7;akt-1. Authors filtered genes by the following criteria: (1) status = OK for wild-type vs. eak-7;akt-1, (2) fold change (FC) >= 1.5 or FC <= 1/1.5 for wild-type vs. eak-7;akt-1 and (3) FDR < 0.05 for at least two separate comparisons.	WBPaper00050801:DAF-12_dauer_regulome
	Potental DAF-12 target genes identified by ChIP-chip analysis performed on strain ALF4 [daf-12	Affymetrix TAS software that computed for each probe estimates of fold enrichment (in linear scale) over hybridization with input DNA. At the same time, TAS calculated for each probe a p-value by applying a Wilcoxon signed rank test. A threshold of 2.5 was selected, which corresponds to probe intensities approximately 2.5 times stronger on the ChIP array than on the Input array. Additional TAS threshold parameters were MinRun=180 bp, MaxGap=300 bp. TAS analysis showed that the selected threshold of 2.5 corresponds approximately to a p-value of 0.01.	WBPaper00040221:DAF-12_target_ALF4
	Genes up-regulated in long-lived daf-12(rh273) are listed including the log2 fold-induction in daf-12(rh273) compared to daf-12(rh61rh411).	Differentially expressed genes identified were identified using SAM using delta 0.987 and FDR 5.3.	WBPaper00027339:daf-12(rh273)_upregulated
	Genes that were regulated by DAF-12 in response to germline loss, identified by comparing differentially expression genes between glp-1(e2141ts, 25C) vs. glp-1(e2141ts, 20C) and daf-12;glp-1(e2141ts, 25C) vs. daf-12;glp-1(e2141ts, 20C).	All lists used an Fs:Ptab 0.01 cutoff.	WBPaper00040412:germline-regulated_daf-12_target
	Transcripts that showed significantly increased expression in PVD neurons isolated from TV22833 wyIs840(ser2prom3::daf-12a(rh273)::gfpnovo2, Pmyo-2::gfp); wyIs592(ser2prom3::myr-gfp), comparing to in PVD neurons isolated from animals that only expressed wyIs592.	Cufflinks 2.2.1	WBPaper00058678:DAF-12_upregulated_PVD
	Genes down-regulated in long-lived daf-12(rh273) are listed including the log2 fold-induction in daf-12(rh273) compared to daf-12(rh61rh411).	Differentially expressed genes identified were identified using SAM using delta 0.987 and FDR 5.3.	WBPaper00027339:daf-12(rh273)_downregulated
	Potental DAF-12 target genes identified by ChIP-chip analysis performed on strain ALF9 [daf-12	Affymetrix TAS software that computed for each probe estimates of fold enrichment (in linear scale) over hybridization with input DNA. At the same time, TAS calculated for each probe a p-value by applying a Wilcoxon signed rank test. A threshold of 2.5 was selected, which corresponds to probe intensities approximately 2.5 times stronger on the ChIP array than on the Input array. Additional TAS threshold parameters were MinRun=180 bp, MaxGap=300 bp. TAS analysis showed that the selected threshold of 2.5 corresponds approximately to a p-value of 0.01.	WBPaper00040221:DAF-12_target_ALF9
	Transcripts that showed significantly decreased expression in PVD neurons isolated from TV22833 wyIs840(ser2prom3::daf-12a(rh273)::gfpnovo2, Pmyo-2::gfp); wyIs592(ser2prom3::myr-gfp), comparing to in PVD neurons isolated from animals that only expressed wyIs592.	Cufflinks 2.2.1	WBPaper00058678:DAF-12_downregulated_PVD

1 Sequence

• Length: 30968

1 Sequence Ontology Term