WormMine

WS294

Intermine data mining platform for C. elegans and related nematodes

Gene :

WormBase Gene ID  ? WBGene00001896 Gene Name  his-22
Sequence Name  ? K06C4.12 Brief Description  his-22 encodes an H2B histone.
Organism  Caenorhabditis elegans Automated Description  Predicted to enable DNA binding activity and protein heterodimerization activity. Predicted to be a structural constituent of chromatin. Predicted to be part of nucleosome. Is an ortholog of several human genes including H2BC3 (H2B clustered histone 3); H2BC5 (H2B clustered histone 5); and H2BC8 (H2B clustered histone 8).
Biotype  SO:0001217 Genetic Position  V :1.67281±
Length (nt)  ? 372
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1 Organism

Name Taxon Id
Caenorhabditis elegans 6239

1 Synonyms

Value
WBGene00001896

Genomics

1 Transcripts

WormMine ID Sequence Name Length (nt) Chromosome Location
Transcript:K06C4.12.1 K06C4.12.1 372   V: 8892559-8892930
 

Other

1 CDSs

WormMine ID Sequence Name Length (nt) Chromosome Location
CDS:K06C4.12 K06C4.12 372   V: 8892559-8892930

28 RNAi Result

WormBase ID
WBRNAi00095723
WBRNAi00025513
WBRNAi00026258
WBRNAi00027343
WBRNAi00064493
WBRNAi00015036
WBRNAi00090826
WBRNAi00047423
WBRNAi00068391
WBRNAi00068392
WBRNAi00068393
WBRNAi00068394
WBRNAi00068395
WBRNAi00068620
WBRNAi00068621
WBRNAi00068622
WBRNAi00068623
WBRNAi00068624
WBRNAi00068871
WBRNAi00025511
WBRNAi00111790
WBRNAi00075452
WBRNAi00096920
WBRNAi00069020
WBRNAi00103363
WBRNAi00081664
WBRNAi00110850
WBRNAi00096980

6 Allele

Public Name
gk963301
gk964351
gk962860
gk964052
gk963442
gk963441

1 Chromosome

WormBase ID Organism Length (nt)
V Caenorhabditis elegans 20924180  

1 Chromosome Location


Feature . Primary Identifier
Start End Strand
WBGene00001896 8892559 8892930 1

3 Data Sets

Name URL
WormBaseAcedbConverter  
GO Annotation data set  
C. elegans genomic annotations (GFF3 Gene)  

1 Downstream Intergenic Region

WormBase ID Name Sequence Name Length (nt) Chromosome Location Organism
intergenic_region_chrV_8892931..8895100   2170 V: 8892931-8895100 Caenorhabditis elegans

42 Expression Clusters

Regulated By Treatment Description Algorithm Primary Identifier
  oocyte proteins identified by two or more unique peptides during proteomics study. In the pooled data set, 1453 C. elegans proteins were identified with a probability >= 0.9 according to ProteinProphet, of which 1165 proteins were identified by more than one unique peptide. WBPaper00038289:oocyte_protein
  Transcripts expressed in neuronal cells, by analyzingfluorescence-activated cell sorted (FACS) neurons. DESeq. False discovry rate (FDR) < 0.1. WBPaper00048988:neuron_expressed
  Transcripts that showed significantly higher expression in somatic gonad precursor cells (SGP) vs. head mesodermal cells (hmc). DESeq2, fold change >= 2, FDR <= 0.01. WBPaper00056826:SGP_biased
  Proteins interacting with NHR-49-GFP according to co-IP and LC-MS. N.A. WBPaper00064071:NHR-49_interacting
  Transcripts that showed significantly decreased expression in daf-16(mgDf50) comparing to in N2 at L1 larva stage. DESeq v1.20.0 was used to analyze differential gene expression. Transcripts with adjusted p-value < 0.05 were considered differentialled expressed. WBPaper00048971:daf-16(mgDf50)_downregulated_L1
  Transcripts that showed significantly increased expression in hira-1(gk835598) comparing to in N2 animals at L4 larva stage. Differential expression analysis was done with Rversion 2.15.3 using DESeq_1.10.1. Fold change > 2, p-value < 0.01. WBPaper00059739:hira-1(gk835598)_upregulated_L4
  Transcripts that showed significantly increased expression in animals lacking P granules by RNAi experiments targeting pgl-1, pgl-3, glh-1 and glh-4, and unc-119-GFP(+), comparing to in control animals, at 2-day post L4 adult hermaphrodite stage. DESeq2, Benjamini-Hochberg multiple hypothesis corrected p-value < 0.05 and fold change > 2. WBPaper00050859:upregulated_P-granule(-)GFP(+)_vs_control_day2-adult
  Transcripts that showed significantly increased expression in animals lacking P granules by RNAi experiments targeting pgl-1, pgl-3, glh-1 and glh-4, and unc-119-GFP(-), comparing to in control animals, at 2-day post L4 adult hermaphrodite stage. DESeq2, Benjamini-Hochberg multiple hypothesis corrected p-value < 0.05 and fold change > 2. WBPaper00050859:upregulated_P-granule(-)GFP(-)_vs_control_day2-adult
  Transcripts that showed significantly increased expression in pgl-1(ct131) animals (isolated from SS0002[pgl-1(ct131)him-3(e1147)], comparing to in control animals SS1174, at 1-day post L4 adult hermaphrodite stage. DESeq2, Benjamini-Hochberg multiple hypothesis corrected p-value < 0.05 and fold change > 2. WBPaper00050859:upregulated_pgl-1(ct131)_vs_control_day1-adult
  Transcripts of coding genes that showed significantly increased expression in muscle. DESeq2 (version 1.24.0). Transcripts with a false-discovery rate adjusted p-value less than 0.05 were considered significantly differentially expressed. WBPaper00062325:muscle_enriched_coding-RNA
  Transcripts that showed significantly increased expression in emb-4(hc60) comparing to in N2. DESeq2 WBPaper00052884:emb-4(hc60)_upregulated
  Transcripts that showed significantly decreased expression in pals-17(syb3980) comparing to in N2 animals at young adult stage. Differential expression analyses were performed using limma-voom in Galaxy, adj p <= 0.05, logFC > 2 WBPaper00065984:pals-17(syb3980)_downregulated
  Transcripts that showed significantly increased expression in animals lacking P granules by RNAi experiments targeting pgl-1, pgl-3, glh-1 and glh-4, and unc-119-GFP(-), comparing to in control animals, at 1-day post L4 adult hermaphrodite stage. DESeq2, Benjamini-Hochberg multiple hypothesis corrected p-value < 0.05 and fold change > 2. WBPaper00050859:upregulated_P-granule(-)GFP(-)_vs_control_day1-adult
  Transcripts that interact with 3xFLAG-DLC-1 as identified by immunoprecipitation followed by RNA sequencing. DESeq2, fold change > 2,, p-value < 0.01 WBPaper00055334:DLC-1_interacting
  Transcripts that showed significantly decreased expression in plp-1(ok2155) adult animals comparing to in wild type. DESeq2. Genes with at least 2-fold upregulation with a raw P value <= 0.05 only are listed. WBPaper00060526:plp-1(ok2155)_downregulated
  Transcripts that showed significantly increased expression in dop-1(vs100); [Pcol-19-UbG76V-GFP] comparing to in control animal odIs77[Pcol-19-UbG76V-GFP] EdgeR with a Benjamini and Hochberg correction. Transcriptions that showed more than 1.5-fold of change in expression, as well as FDR-adjusted q-value < 0.01 were considered differentially expressed. WBPaper00049635:dop-1(vs100)_upregulated
Fungi: Myzocytiopsis humicola extract Transcripts that showed significantly decreased expression in N2 L4 larva animals after 4 hours of exposure to oomycete Myzocytiopsis humicola extract. Differentially expressed genes as determined by Kallisto and Sleuth (pval < 0.01, qval < 0.1, fold change > 2). WBPaper00065995:M.humicola-extract_downregulated_N2
  Transcripts that showed significantly increased expression in nhr-8(hd117) comparing to in N2. Differentially expressed genes were identified using Sleuth (version 0.30.0). WBPaper00062446:nhr-8(hd117)_upregulated
  Genes that showed increased mRNA expression in emr-1(gk119) comparing to N2. Library siggenes in R was applied to select the differentially expressed loci using the false discovery rate-based SAM method. Genes presenting a delta value > 4 were considered to be significantly altered. WBPaper00044786:emr-1(gk119)_upregulated
  Transcripts that showed significantly increased expression in animals treated with 25ug per mL tunicamycin for 4 hours, comparing to control animals. Fold-change > 1.5, ANOVA P values < 0.05. WBPaper00055482:Tunicamycin_upregulated
heat shock: 33C 30min Transcripts that showed significantly increased expression in animals injected with control vector, after heat shock at 33 C for 30min, comparing to without heat shock. Transcripts that were differentially expressed in different conditions, compared to the hsf-1(+);-HS control, were determined with CuffDiff, which uses the Benjamini-Hochberg correction for multiple testing to obtain the q-value (the FDR-adjusted the p-value). WBPaper00049942:HeatShock_upregulated_hsf-1(+)
  Genes that were considered as putative WNT pathway targets because they have higher expression in WNT overactivated (by delNTbar-1 overexpression in huIs10) animals comparing to in N2. Genes that were considered as putative WNT pathway targets based on the following criteria. (1) an average fold change of 1.5-fold or more in deIs10; huIs1 (Wnt pathway overactivation) compared to control (N2); (2) present and increased calls in at least two of three biological replicates; (3) ANOVA P <=0.05; and (4) ratio of average fold change >= 1.5-fold between Wnt pathway overactivated (deIs10; huIs1) and underactivated (deIs10; deIs26) conditions. WBPaper00046887:WNT-target
Temperature shift: 15C vs 20 C. Proteins that showed significantly decreased expression in CF512 (fer-15(b26)II;fem-1(hc17)IV) treated with empty RNAi vector at 15C comparing to at 20C. Two-tailed Student's t-test (n=3, P-value <0.05 was considered significant). WBPaper00057288:Cold_downregulated_protein
  Proteins interacting with TEBP-1-FLAG-GFP (referred as DTN-2 by authors) during immunoprecipitation. N.A. WBPaper00060908:TEBP-1_interacting
  Proteins interacting with TEBP-2-FLAG-GFP (referred as DTN-2 by authors) during immunoprecipitation. N.A. WBPaper00060908:TEBP-2_interacting
  Genes that are significantly up regulated after 24 hour exposure to H2S. Data were RMA normalized and probe-level data were Summarized with the NimbleScan software. Genes with weak signal intensity across all groups and those with low variability across samples were excluded from further analysis. Each H2S-treated sample was statistically compared to a matched untreated control using the Bioconductor package limma. The false discovery rate (FDR) method of Benjamini and Hochberg was used to adjust p-values for multiple testing. An adjusted p-value <= 0.05 was used to define differential expression. WBPaper00040285:H2S_24hr_upregulated
  Transcripts that showed significantly increased expression in hira-1(gk835598) comparing to in N2 animals at adult stage. Differential expression analysis was done with Rversion 2.15.3 using DESeq_1.10.1. Fold change > 2, p-value < 0.01. WBPaper00059739:hira-1(gk835598)_upregulated_adult
Bacteria: P.aeruginosa strain PA14 Transcripts that showed significantly increased expression after infection by P. aeruginosa PA14. Ballgown was used to calculate differential expression of genes using FPKM data and to generate tables with fold change and P values. Genes were shortlisted with a cutoff of 2-fold change and P values of less than 0.05. WBPaper00059754:P.aeruginosa_PA14_upregulated
  Transcripts that showed significantly increased expression in animals with pan-neuronal expression of human alpha-Synuclein(A53T) (aex-3p-alpha-syn (A53T)), comparing to in N2 animals. edgeR p-value < 0.01 and fold change > 4. WBPaper00061613:human-alpha-Synuclein(A53T)_upregulated
  Proteins associated with lipid droplets, according to proteomics study. The search results were filtered with Xcorr versus Charge values of, Xcorr (+2) > 2.5, and Xcorr (+3) > 3.5. Two times peptide FDR: FDR1 = 0.434%, FDR2 = 0.426%. peptide mass accuracy < 5ppm, SP score > 500, RSp < 5. distinct peptides >= 2, misscleavages 2. WBPaper00040965:lipid-droplet_associated_protein

1 Expression Patterns

Remark Reporter Gene Primary Identifier Pattern Subcellular Localization
    Expr1153752 Developmental gene expression time-course. Raw data can be downloaded from ftp://caltech.wormbase.org/pub/wormbase/datasets-published/hashimshony2015  

4 GO Annotation

Annotation Extension Qualifier
  enables
  part_of
  enables
  enables

0 Homologues

1 Locations


Feature . Primary Identifier
Start End Strand
WBGene00001896 8892559 8892930 1

4 Ontology Annotations

Annotation Extension Qualifier
  enables
  part_of
  enables
  enables

0 Regulates Expr Cluster

1 Sequence

Length
372

1 Sequence Ontology Term