WormMine

WS294

Intermine data mining platform for C. elegans and related nematodes

Gene :

WormBase Gene ID  ? WBGene00003210 Gene Name  mel-28
Sequence Name  ? C38D4.3 Brief Description  mel-28 encodes a large (1,784-residue) protein required for nuclearenvelope assembly; MEL-28 is highly divergent from, but orthologous tohuman AT-hook-containing transcription factor 1 (AHCTF1); MEL-28 isfound in nuclear pore complexes (NPCs) during interphase, kinetochoresearly in mitosis, and chromatin later in mitosis; mel-28 mutants werefirst identified in a screen for genes involved in cell division in theearly embryo; mel-28 mutants or mel-28(RNAi) animals have no (or poorlyvisible) pronuclei and nuclear morphology generally, along with a weakmitotic spindle, aberrant chromatin segregation, and abnormallydistributed nuclear envelope (NE) proteins and NPCs; MEL-28 is neededfor normal localization of LMN-1 and EMR-1, and appears to be a stablestructural component of the NE; maternal MEL-28 is needed for embryonicdevelopment.
Organism  Caenorhabditis elegans Automated Description  Enables molecular adaptor activity. Involved in mitotic sister chromatid segregation and nuclear pore complex assembly. Located in kinetochore; nuclear envelope; and nucleoplasm. Part of nuclear pore.
Biotype  SO:0001217 Genetic Position  III :-2.54057 ±0.008695
Length (nt)  ? 6312
Quick Links:
 

1 Organism

Name Taxon Id
Caenorhabditis elegans 6239

1 Synonyms

Value
WBGene00003210

Genomics

1 Transcripts

WormMine ID Sequence Name Length (nt) Chromosome Location
Transcript:C38D4.3.1 C38D4.3.1 5463   III: 4786080-4792391
 

Other

1 CDSs

WormMine ID Sequence Name Length (nt) Chromosome Location
CDS:C38D4.3 C38D4.3 5355   III: 4786081-4786171

38 RNAi Result

WormBase ID
WBRNAi00065559
WBRNAi00065561
WBRNAi00065562
WBRNAi00065560
WBRNAi00091607
WBRNAi00067791
WBRNAi00068029
WBRNAi00024778
WBRNAi00064812
WBRNAi00113433
WBRNAi00065349
WBRNAi00065350
WBRNAi00077940
WBRNAi00065564
WBRNAi00113699
WBRNAi00065343
WBRNAi00065344
WBRNAi00065345
WBRNAi00065346
WBRNAi00065563
WBRNAi00065347
WBRNAi00076205
WBRNAi00069011
WBRNAi00069012
WBRNAi00002428
WBRNAi00068763
WBRNAi00068762
WBRNAi00111628
WBRNAi00007772
WBRNAi00042157

71 Allele

Public Name
t1578
t1684
WBVar01825489
WBVar00052346
WBVar01903721
gk473637
WBVar02034194
WBVar00269726
WBVar01644287
WBVar00602628
gk955022
WBVar01893297
WBVar01644286
gk173432
gk173431
gk173434
gk173433
gk173428
gk173430
gk173429
gk173440
gk173439
tm2434
gk173441
gk173436
gk173435
gk173438
gk173437
WBVar01445461
WBVar01445459

1 Chromosome

WormBase ID Organism Length (nt)
III Caenorhabditis elegans 13783801  

1 Chromosome Location


Feature . Primary Identifier
Start End Strand
WBGene00003210 4786080 4792391 1

3 Data Sets

Name URL
WormBaseAcedbConverter  
GO Annotation data set  
C. elegans genomic annotations (GFF3 Gene)  

1 Downstream Intergenic Region

WormBase ID Name Sequence Name Length (nt) Chromosome Location Organism
intergenic_region_chrIII_4792392..4793085   694 III: 4792392-4793085 Caenorhabditis elegans

161 Expression Clusters

Regulated By Treatment Description Algorithm Primary Identifier
  Transcripts of coding genes that showed significantly decreased expression in muscle. DESeq2 (version 1.24.0). Transcripts with a false-discovery rate adjusted p-value less than 0.05 were considered significantly differentially expressed. WBPaper00062325:muscle_depleted_coding-RNA
  Transcripts expressed in neuronal cells, by analyzingfluorescence-activated cell sorted (FACS) neurons. DESeq. False discovry rate (FDR) < 0.1. WBPaper00048988:neuron_expressed
  Genes that showed expression levels higher than the corresponding reference sample (embryonic 24hr reference). A Mann-Whitney U test with an empirical background model and FDR correction for multiple testing was used to detect expressed transcripts (Benjamini and Hochberg 1995). Genes and TARs with an FDR <= 0.05 were reported as expressed above background. Authors detected differentially expressed transcripts using a method based on linear models. Genes and TARs were called differentially expressed if the FDR was <= 0.05 and the fold change (FC) >= 2.0. To more strictly correct for potential false-positives resulting from multiple sample comparisons, authors divided individual FDR estimates by the number of samplesor sample comparisons, respectively. This resulted in an adjusted FDR of 1.3 * 0.0001 for expression above background and of 7.4 * 0.0001 for differential expression. Authors called genes selectively enriched in a given tissue if they met the following requirements: (1) enriched expression in a given tissue (FDR <= 0.05 and FC >= 2.0), (2) fold change versus reference among the upper 40% of the positive FC range observed for this gene across all tissues, and (3) fold-change entropy among the lower 40% of the distribution observed for all genes. WBPaper00037950:all-neurons_L1-larva_expressed
adult vs dauer larva Transcripts that showed differential expression in adult vs dauer lava in N2 animals at 20C. N.A. WBPaper00050488:adult_vs_dauer_regulated_N2_20C
  mRNAs that showed decreased expression in 1 cell mebryo comparing to in oocyte, according to RNAseq analysis. Gaussian error propagation. As cutoff for the up-regulated genes authors used log2 fold change > 1 and P < 0.05 and as cutoff for the down-regulated genes authors used log2 fold change < -1 and P < 0.05. WBPaper00045420:fertilization_downregulated_transcript
  Genes that showed expression levels higher than the corresponding reference sample (embryonic 24hr reference). A Mann-Whitney U test with an empirical background model and FDR correction for multiple testing was used to detect expressed transcripts (Benjamini and Hochberg 1995). Genes and TARs with an FDR <= 0.05 were reported as expressed above background. Authors detected differentially expressed transcripts using a method based on linear models. Genes and TARs were called differentially expressed if the FDR was <= 0.05 and the fold change (FC) >= 2.0. To more strictly correct for potential false-positives resulting from multiple sample comparisons, authors divided individual FDR estimates by the number of samplesor sample comparisons, respectively. This resulted in an adjusted FDR of 1.3 * 0.0001 for expression above background and of 7.4 * 0.0001 for differential expression. Authors called genes selectively enriched in a given tissue if they met the following requirements: (1) enriched expression in a given tissue (FDR <= 0.05 and FC >= 2.0), (2) fold change versus reference among the upper 40% of the positive FC range observed for this gene across all tissues, and (3) fold-change entropy among the lower 40% of the distribution observed for all genes. WBPaper00037950:AVE-neuron_L1-larva_expressed
  Genes that showed expression levels higher than the corresponding reference sample (embryonic 24hr reference). A Mann-Whitney U test with an empirical background model and FDR correction for multiple testing was used to detect expressed transcripts (Benjamini and Hochberg 1995). Genes and TARs with an FDR <= 0.05 were reported as expressed above background. Authors detected differentially expressed transcripts using a method based on linear models. Genes and TARs were called differentially expressed if the FDR was <= 0.05 and the fold change (FC) >= 2.0. To more strictly correct for potential false-positives resulting from multiple sample comparisons, authors divided individual FDR estimates by the number of samplesor sample comparisons, respectively. This resulted in an adjusted FDR of 1.3 * 0.0001 for expression above background and of 7.4 * 0.0001 for differential expression. Authors called genes selectively enriched in a given tissue if they met the following requirements: (1) enriched expression in a given tissue (FDR <= 0.05 and FC >= 2.0), (2) fold change versus reference among the upper 40% of the positive FC range observed for this gene across all tissues, and (3) fold-change entropy among the lower 40% of the distribution observed for all genes. WBPaper00037950:bodywall-muscle_L1-larva_expressed
  Genes that were upregulated in lin-15B(n744). For each gene in each microarray hybridization experiment, the ratio of RNA levels from the two samples was transformed into a log2 value and the mean log2 ratio was calculated. The log2 ratios were normalized by print-tip Loess normalization (Dudoit and Yang, 2002). All genes with a false discovery rate of <= 5% (q <= 0.05) (Storey and Tibshirani, 2003) and a mean fold-change ratio of >= 1.5 were selected for further analysis. WBPaper00038168:lin-15B(n744)_upregulated
  Transcripts that showed significantly increased expression after animals were treated with 100uM Rapamycin and 50mM Metformin from day 1 to day 3 adult hermaphradite. DESeq2(v1.14.1), fold change > 2, p-value < 0.05 WBPaper00055354:Rapamycin-Metformin_upregulated
  Proteins interacting with NHR-49-GFP according to co-IP and LC-MS. N.A. WBPaper00064071:NHR-49_interacting
  Transcripts expressed in hypodermis, according to PAT-Seq analysis using Pdpy-7-GFP-3XFLAG mRNA tagging. Cufflinks FPKM value >=1. WBPaper00050990:hypodermis_expressed
  Transcripts expressed in intestine, according to PAT-Seq analysis using Pges-1-GFP-3XFLAG mRNA tagging. Cufflinks FPKM value >=1. WBPaper00050990:intestine_expressed
  Maternal class (M): genes that are called present in at least one of the three PC6 replicates. A modified Welch F statistic was used for ANOVA. For each gene, regressed error estimates were substituted for observed error estimates. The substitution is justified by the lack of consistency among the most and least variable genes at each time point. Regressed error estimates were abundance-dependent pooled error estimates that represented a median error estimate from a window of genes of similar abundance to the gene of interest. A randomization test was used to compute the probability Pg of the observed F statistic for gene g under the null hypothesis that developmental time had no effect on expression. P-values were not corrected for multiple testing. [cgc5767]:expression_class_M
  Transcripts expressed in NMDA neuron, according to PAT-Seq analysis using Pnmr-1-GFP-3XFLAG mRNA tagging. Cufflinks FPKM value >=1. WBPaper00050990:NMDA-neuron_expressed
  Transcripts expressed in seam cells, according to PAT-Seq analysis using Pgrd-10-GFP-3XFLAG mRNA tagging. Cufflinks FPKM value >=1. WBPaper00050990:seam_expressed
Bacteria infection: Bacillus thuringiensis mRNAs that showed significantly decreased expression after pathogenic bacteria Bacillus thuringiensis infections comparing to non pathogenic BT (BT247(1 to 10 mix) vs BT407 12h), according to RNAseq. Cuffdiff, ajusted p-value < 0.01. WBPaper00046497:B.thuringiensis_0.1mix_downregulated_12h
Bacteria infection: Bacillus thuringiensis mRNAs that showed significantly decreased expression after pathogenic bacteria Bacillus thuringiensis infections comparing to non pathogenic BT (BT247(1 to 2 mix) vs BT407 6h), according to RNAseq. Cuffdiff, ajusted p-value < 0.01. WBPaper00046497:B.thuringiensis_0.5mix_downregulated_6h
Bacteria infection: Bacillus thuringiensis mRNAs that showed significantly decreased expression after pathogenic bacteria Bacillus thuringiensis infections comparing to non pathogenic BT (BT247(1 to 2 mix) vs BT407 12h), according to RNAseq. Cuffdiff, ajusted p-value < 0.01. WBPaper00046497:B.thuringiensis_0.5mix_downregulated_12h
  Transcripts that showed significantly decreased expression in hsp-6(mg585) comparing to in N2 at L4 larva stage. EdgeR, fold change > 2, FDR < 0.001. WBPaper00056290:hsp-6(mg585)_downregulated
  Transcripts that showed significantly increased expression in animals exposed to 400uM tamoxifen from L1 to L4 larva stage. DEseq2, fold change > 2 WBPaper00064505:tamoxifen_upregulated
  Transcripts that showed significantly changed expression in 6-day post-L4 adult hermaphrodite comparing to in 1-day post L4 adult hermaphrodite animals. Sleuth WBPaper00051558:aging_regulated
  Transcripts that showed significantly altered expression after 24 hour exposure to stavudine (d4T) starting at L1 lava stage. DESeq WBPaper00053302:stavudine_24h_regulated
  Genes down regulated by mir-243(n4759). RNAs that changed at least 2-fold with a probability of p > 0.05 in three biological replicates were considered differentially regulated between wild-type and mir-243. WBPaper00036130:mir-243_down_regulated
25C vs. 20C Transcripts that showed significantly increased expression in 1-day post L4 adult hermaphrodite N2 grown at 25C, comparing to in N2 animals grown at 20C. CuffDiff, fold change > 2. WBPaper00065096:25C_vs_20C_upregulated
  Transcripts that showed significantly increased expression in 10-days post L4 adult hermaphrodite N2 grown at 20C, comparing to in 1-day post L4 adult hermaphrodite N2 animals grown at 20C. CuffDiff, fold change > 2. WBPaper00065096:Day10_vs_Day1_upregulated
  Transcripts that showed significantly decreased expression in tetraploid N2 comparing to diploid N2 animals at L4 larva stage. DESeq2 R package (1.20.0), fold change > 2, and FDR < 0.05. WBPaper00066110:tetraploid_vs_diploid_downregulated
  Genes that showed expression levels higher than the corresponding reference sample (L2 all cell reference). A Mann-Whitney U test with an empirical background model and FDR correction for multiple testing was used to detect expressed transcripts (Benjamini and Hochberg 1995). Genes and TARs with an FDR <= 0.05 were reported as expressed above background. Authors detected differentially expressed transcripts using a method based on linear models. Genes and TARs were called differentially expressed if the FDR was <= 0.05 and the fold change (FC) >= 2.0. To more strictly correct for potential false-positives resulting from multiple sample comparisons, authors divided individual FDR estimates by the number of samplesor sample comparisons, respectively. This resulted in an adjusted FDR of 1.3 * 0.0001 for expression above background and of 7.4 * 0.0001 for differential expression. Authors called genes selectively enriched in a given tissue if they met the following requirements: (1) enriched expression in a given tissue (FDR <= 0.05 and FC >= 2.0), (2) fold change versus reference among the upper 40% of the positive FC range observed for this gene across all tissues, and (3) fold-change entropy among the lower 40% of the distribution observed for all genes. WBPaper00037950:coelomocytes_L2-larva_expressed
  Genes that showed expression levels higher than the corresponding reference sample (L2 all cell reference). A Mann-Whitney U test with an empirical background model and FDR correction for multiple testing was used to detect expressed transcripts (Benjamini and Hochberg 1995). Genes and TARs with an FDR <= 0.05 were reported as expressed above background. Authors detected differentially expressed transcripts using a method based on linear models. Genes and TARs were called differentially expressed if the FDR was <= 0.05 and the fold change (FC) >= 2.0. To more strictly correct for potential false-positives resulting from multiple sample comparisons, authors divided individual FDR estimates by the number of samplesor sample comparisons, respectively. This resulted in an adjusted FDR of 1.3 * 0.0001 for expression above background and of 7.4 * 0.0001 for differential expression. Authors called genes selectively enriched in a given tissue if they met the following requirements: (1) enriched expression in a given tissue (FDR <= 0.05 and FC >= 2.0), (2) fold change versus reference among the upper 40% of the positive FC range observed for this gene across all tissues, and (3) fold-change entropy among the lower 40% of the distribution observed for all genes. WBPaper00037950:GABAergic-motor-neurons_L2-larva_expressed
  Transcripts that showed significantly increased expression in ilc-17.1(syb5296) comparing to in N2 animals at L4 larva stage. DESeq2, fold change > 2, FDR < 0.05. WBPaper00066594:ilc-17.1(syb5296)_upregulated
  Transcripts that showed significantly increased expression in pry-1(mu38) animals comparing to in N2 at L1 larva stage. DESeq, FDR < 0.05 WBPaper00055626:pry-1(mu38)_upregulated

16 Expression Patterns

Remark Reporter Gene Primary Identifier Pattern Subcellular Localization
Visualization of GFP::MEL-28 in living embryos and immunofluorescence labeling show the same localization patterns, which are abolished in mel-28(RNAi) animals.   Expr4321   MEL-28 shuttles dynamically between the nuclear periphery and the kinetochore during the cell cycles of early embryogenesis. During meiosis, MEL-28 is present on the nuclear periphery in the oocyte, on the condensing oocyte chromosomes, and on meiotic chromosomes (polar bodies) in the fertilized embryo. During interphase of the first mitotic cycle, MEL-28 is present at the nuclear periphery and as puncta within the nucleus. On the nuclear periphery, MEL-28 localization overlaps with the interior rim of nuclear-envelope marker mAB414 (which in C. elegans recognizes primarily nucleoporins NPP-9 [Nup358] and NPP-10 [Nup98/96]. As the pronuclei meet, each nucleus shows a dynamic reorganization of the internal puncta. By metaphase, the scattered dots resolve into pairs of stripes on either side of the metaphase plate, a pattern indicative of holocentric kinetochore localization.
    Expr4322   MEL-28 was expressed in embryos as well as in all larval stages and in adults. Immunolocalization of MEL-28 in several tissues revealed a nuclear-rim localization typical for NE or NPC proteins. MEL-28 was localized to chromatin during mitosis. The localization of MEL-28 within the NE was then analyzed by immuno-gold TEM. As expected, the highest density of gold particles was observed at the NE, but particles were also observed in the nucleoplasm and the cytoplasm. Particles found in the cytoplasm most likely reflect a cross reactivity of the MEL-28 antibody because a similar density of gold particles was observed in the cytoplasm of mel-28(t1684) embryos. No gold particles were observed in the nuclei or at the nuclear periphery of mutant embryos. Of the particles associated with the NE in the wild-types, 95.0% (152/160) were found at NPCs. No accumulation at the outer nuclear membrane was detected. MEL-28 enrichment at NPCs was also detected in gonad nuclei. Immunofluorescence analysis revealed that MEL-28 began to localize to the condensing chromosomes before complete disappearance of mAb414 staining from the nuclear rim at prophase and began to appear as two lines parallel to the metaphase plate. During late anaphase, MEL-28 signal colocalized with the decondensing chromosomes, and the mAb414 signal reaccumulated to the reforming NE during telophase. Immunolocalization of MEL-28 in embryos expressing GFP-HIM-10 revealed an overlapping localization of both proteins parallel to the metaphase plate within the mitotic spindle. Only a few kinetochore microtubules remained visible after 30 s on ice, and after 60 s, microtubules were not detectable. At both time points, MEL-28 staining remained clearly visible along the condensed and aligned chromosomes, indicating that MEL-28 localizes to kinetochores during mitosis. MEL-28 localization changes rapidly at late anaphase and telophase. MEL-28 initially spreads over the entire chromatin surface then rapidly relocalizes to the NE. This distribution would be consistent with MEL-28's playing a role in the interactions that occur between chromatin and the assembling NE and NPCs late in mitosis.
    Expr4323   To investigate the dynamics of MEL-28 in living embryos, GFP-MEL-28 was compared with known NE proteins fused to G/YFP using confocal time-lapse microscopy. Like endogenous MEL-28, which was localized by immunostaining, GFP-MEL-28 was mostly concentrated at the nuclear periphery but was also detected in the nucleoplasm and associated with condensing chromosomes when cells entered mitosis. This pattern was indistinguishable from that of YFP-Nup107, which also localizes to kinetochores and is recruited very early during NE assembly. Consistent with previous reports, GFP-Nup155 associated with the NE approximately 60 s after anaphase onset when the integral NE protein LEM-2 began to concentrate around chromosomes. Thus, MEL-28 is present on chromatin before NE reassembly and earlier than most other NE components.
    Expr1031516 Tiling arrays expression graphs  
    Expr2013518 Single cell embryonic expression. Only cell types with an expression fraction of greater 0.2 of the maximum expressed fraction are labeled (Full data can be downloaded from http://caltech.wormbase.org/pub/wormbase/datasets-published/packer2019/). The colors represent the broad cell class to which the cell type has been assigned. The size of the point is proportional to the log2 of the numbers of cells in the dataset of that cell type. Interactive visualizations are available as a web app (https://cello.shinyapps.io/celegans/) and can also be installed as an R package (https://github.com/qinzhu/VisCello.celegans).  
    Expr10361 Inferred expression. EPIC dataset. http://epic.gs.washington.edu/ Large-scale cellular resolution compendium of gene expression dynamics throughout development. This reporter was inferred to be expressing in this cell or one of its embryonic progenitor cells as described below. To generate a compact description of which cells express a particular reporter irrespective of time, the authors defined a metric "peak expression" for each of the 671 terminal ("leaf") cells born during embryogenesis. For each of these cells, the peak expression is the maximal reporter intensity observed in that cell or any of its ancestors; this has the effect of transposing earlier expression forward in time to the terminal set of cells. This metric allows straightforward comparisons of genes' cellular and lineal expression overlap, even when the expression occurs with different timing and despite differences in the precise time point that curation ended in different movies, at the cost of ignoring the temporal dynamics of expression, a topic that requires separate treatment. For simplicity, the authors use the term "expressing cells" to mean the number of leaf cells (of 671) with peak expression greater than background (2000 intensity units) and at least 10% of the maximum expression in that embryo. Quantitative expression data for all cells are located here: ftp://caltech.wormbase.org/pub/wormbase/datasets-published/murray2012/  
    Expr14727   GFP fusions of five tested Y-complex subunits (npp-2, npp-6, mel-28, and npp-18) revealed localization to the cup-like kinetochores and linear elements; for one of them, MEL-28, we confirmed co-localization with the outer kinetochore component KNL-1. All of the tested GFP fusions also localized to the periphery of interphase nuclei and to mitotic kinetochores in embryos.
    Expr1146155 Developmental gene expression time-course. Raw data can be downloaded from ftp://caltech.wormbase.org/pub/wormbase/datasets-published/hashimshony2015  
    Expr12964   Using CRISPR-Cas9 technology, the authors generated a GFP knock-in mel-28 allele to analyze the expression of endogenous MEL-28 by live microscopy. Similar to the observations with antibodies against MEL-28, GFP::MEL-28 localized to the nuclear envelope (NE) in all cell types during embryonic and larval development and in adults. Thus, they conclude that MEL-28 is ubiquitously expressed throughout C. elegans development. MEL-28 strongly accumulated on condensed oocyte chromosomes. In the -4 oocyte, MEL-28 localized to the NE and was absent from condensed chromosomes. In the -3 and -2 oocytes MEL-28 gradually moved away from the NE and accumulated uniformly on meiotic chromosomes. Later, in the -1 oocyte MEL-28 redistributed to cover the surface of meiotic chromosomes, in some cases completely enclosing the chromosomes and in other cases similar to the 'cup-shaped' localization of kinetochore proteins, such as KNL-1 and KNL-3. The association of MEL-28 with chromosomes persisted throughout meiosis I and II until pronuclear formation ~30 minutes after germinal vesicle breakdown. During interphase full- length MEL-28 was mainly localized to the NE but was also found in the nucleoplasm. In prophase and prometaphase, MEL-28 left the NE before complete NE breakdown and associated to the condensing chromosomes. By metaphase, MEL-28 appeared as two lines parallel to the metaphase plate, resembling the characteristic pattern of holocentric kinetochore proteins, and less abundantly to the area of the mitotic spindle. During anaphase, MEL-28 associated to decondensing chromosomes, and re-localized to reforming NE in telophase.
    Expr2031752 Single cell embryonic expression. Only cell types with an expression fraction of greater 0.2 of the maximum expressed fraction are labeled (Full data can be downloaded from http://caltech.wormbase.org/pub/wormbase/datasets-published/packer2019/). The colors represent the broad cell class to which the cell type has been assigned. The size of the point is proportional to the log2 of the numbers of cells in the dataset of that cell type. Interactive visualizations are available as a web app (https://cello.shinyapps.io/celegans/) and can also be installed as an R package (https://github.com/qinzhu/VisCello.celegans).  
    Expr14734   GFP fusions of five tested Y-complex subunits (npp-2, npp-6, mel-28, and npp-18) revealed localization to the cup-like kinetochores and linear elements; for one of them, MEL-28, we confirmed co-localization with the outer kinetochore component KNL-1. All of the tested GFP fusions also localized to the periphery of interphase nuclei and to mitotic kinetochores in embryos.
    Expr14735   GFP fusions of five tested Y-complex subunits (npp-2, npp-6, mel-28, and npp-18) revealed localization to the cup-like kinetochores and linear elements; for one of them, MEL-28, we confirmed co-localization with the outer kinetochore component KNL-1. All of the tested GFP fusions also localized to the periphery of interphase nuclei and to mitotic kinetochores in embryos.
    Expr14740   MEL-28 was detected on chromosomes throughout anaphase.
    Expr14741   A functional GFP::MEL-28 fusion co-localizes with outer kinetochore components, such as KNL-1, during prometaphase and metaphase of meiosis I
    Expr1010769 Developmental gene expression time-course. Raw data can be downloaded from ftp://caltech.wormbase.org/pub/wormbase/datasets-published/levin2012  
    Expr3666   Shuttles between nuclear periphery during interphase onto chromosomes during mitosis (double line at metaphase suggests kinetochore localization).

24 GO Annotation

Annotation Extension Qualifier
  involved_in
  involved_in
  involved_in
  involved_in
  enables
  involved_in
  enables
  located_in
existence_overlaps(GO:0000093) located_in
  located_in
  located_in
  located_in
existence_overlaps(GO:0000088)|existence_overlaps(GO:0000089)|existence_overlaps(GO:0000090) located_in
  involved_in
  located_in
  part_of
  part_of
  located_in
  located_in
existence_overlaps(GO:0051329) located_in
  located_in
  located_in
  located_in
existence_overlaps(GO:0051329) part_of

0 Homologues

1 Locations


Feature . Primary Identifier
Start End Strand
WBGene00003210 4786080 4792391 1

24 Ontology Annotations

Annotation Extension Qualifier
  involved_in
  involved_in
  involved_in
  involved_in
  enables
  involved_in
  enables
  located_in
existence_overlaps(GO:0000093) located_in
  located_in
  located_in
  located_in
existence_overlaps(GO:0000088)|existence_overlaps(GO:0000089)|existence_overlaps(GO:0000090) located_in
  involved_in
  located_in
  part_of
  part_of
  located_in
  located_in
existence_overlaps(GO:0051329) located_in
  located_in
  located_in
  located_in
existence_overlaps(GO:0051329) part_of

0 Regulates Expr Cluster

1 Sequence

Length
6312

1 Sequence Ontology Term