WormMine: Gene WBGene00194809

• WormBase Gene ID: WBGene00194809
• Gene Name: mir-2218.1
• Sequence Name: W09G3.10
• Organism: Caenorhabditis elegans
• Automated Description: Is affected by alg-5 and ilc-17.1 based on RNA-seq studies. Is affected by nicotine based on quantitative PCR and RNA-seq studies.
• Biotype: SO:0001265
• Genetic Position: I :21.9322±
• Length (nt): 60

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Synonyms

• Value: WBGene00194809

Genomics

2 Transcripts

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
Transcript:W09G3.10a	W09G3.10a	23	I: 13812596-13812618
Transcript:W09G3.10b	W09G3.10b	23	I: 13812633-13812655

Other

0 CDSs

0 RNAi Result

5 Allele

• gk963849
• gk964175
• gk962681
• gk963947
• gk788798

1 Chromosome

• WormBase ID: I
• Organism: Caenorhabditis elegans
• Length (nt): 15072434

1 Chromosome Location

• Feature . Primary Identifier: WBGene00194809
• Start: 13812596
• End: 13812655
• Strand: 1

2 Data Sets

• WormBaseAcedbConverter
• C. elegans genomic annotations (GFF3 Gene)

0 Downstream Intergenic Region

6 Expression Clusters

Regulated By Treatment	Description	Algorithm	Primary Identifier
	Transcripts that showed significantly increased expression in ilc-17.1(syb5296) comparing to in N2 animals at L4 larva stage.	DESeq2, fold change > 2, FDR < 0.05.	WBPaper00066594:ilc-17.1(syb5296)_upregulated
	MicroRNAs that showed significantly decreased expression in alg-5(ram2), comparing to in N2.	DESeq2, Fold change > 1.5.	WBPaper00051404:alg-5(ram2)_downregulated_miRNA
	miRNA that showed increased expression after 20mM nicotine treatment	Statistical analysis was based on t-test for independent samples via SPSS. The results were further narrowed based on two criteria. Only the genes whose expression changed with a P < 0.05 and a fold change > 0.5, when compared to control, were considered as differentially expressed. Fold change was calculated as 2**(DeltaCt(treatment)-DeltaCt(control))-1. DeltaCt = Ct(miRNA)-Ct(aveMRNA).	WBPaper00042480:20mM_nicotine_upregulated
	MicroRNAs that showed significantly decreased expression in alg-5(tm1163), comparing to in N2.	DESeq2, Fold change > 1.5.	WBPaper00051404:alg-5(tm1163)_downregulated_miRNA
	miRNAs in F1 generation up regulated by exposure to 20uM and 20mM nicotine during F0 generation.	Statistical analysis was based on t-test for independent samples via SPSS to compare each of the nicotine treatments with control (i.e. low vs. control and high vs. control). Welch test correction was performed to account for unequal variance in each of the compared groups (control and treatment). In addition, to increase the statistical stringency, miRNAs differential expression was deemed significant only if it fulfilled two criteria: p value , 0.05 and expression changed by at least 50% relative to control.	WBPaper00049006:nicotine_upregulated_F1
	miRNAs in F2 generation up regulated by exposure to 20uM and 20mM nicotine during F0 generation.	Statistical analysis was based on t-test for independent samples via SPSS to compare each of the nicotine treatments with control (i.e. low vs. control and high vs. control). Welch test correction was performed to account for unequal variance in each of the compared groups (control and treatment). In addition, to increase the statistical stringency, miRNAs differential expression was deemed significant only if it fulfilled two criteria: p value , 0.05 and expression changed by at least 50% relative to control.	WBPaper00049006:nicotine_upregulated_F2

0 Expression Patterns

0 GO Annotation

0 Homologues

1 Locations

• Feature . Primary Identifier: WBGene00194809
• Start: 13812596
• End: 13812655
• Strand: 1

0 Ontology Annotations

0 Regulates Expr Cluster

1 Sequence

• Length: 60

1 Sequence Ontology Term