WormMine: Gene WBGene00003283

• WormBase Gene ID: WBGene00003283
• Gene Name: mir-55
• Sequence Name: F09A5.6
• Brief Description: mir-55 encodes a microRNA (miRNA), a small noncoding RNA that is likely involved in post-transcriptional regulation of gene expression; miR-55 is expressed at high levels during all developmental stages and in glp-4 mutant hermaphrodites lacking a germline; miR-55 is also detected in him-8 mutants, starved L1 larvae, and dauer larvae; miR-55 does not appear to have orthologs in other species, and the targets of miR-55 are not yet known.
• Organism: Caenorhabditis elegans
• Automated Description: Expressed in several structures, including excretory system; hypodermis; neurons; and seam cell.
• Biotype: SO:0001265
• Genetic Position: :±
• Length (nt): 98

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Synonyms

• Value: WBGene00003283

Genomics

2 Transcripts

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
Transcript:F09A5.6b	F09A5.6b	23	X: 13144842-13144864
Transcript:F09A5.6a	F09A5.6a	22	X: 13144881-13144902

Other

0 CDSs

1 RNAi Result

• WBRNAi00113349

11 Allele

• gk964260
• gk964029
• gk962707
• gk964028
• gk963810
• gk963581
• tm11275
• nDf58
• gk501859
• ok5470
• gk501860

1 Chromosome

• WormBase ID: X
• Organism: Caenorhabditis elegans
• Length (nt): 17718942

1 Chromosome Location

• Feature . Primary Identifier: WBGene00003283
• Start: 13144827
• End: 13144924
• Strand: -1

2 Data Sets

• WormBaseAcedbConverter
• C. elegans genomic annotations (GFF3 Gene)

0 Downstream Intergenic Region

22 Expression Clusters

Regulated By Treatment	Description	Algorithm	Primary Identifier
	Neuronally enriched transcripts according to a comparison of neuronal nuclei IP samples to total nuclei using isolation of nuclei from tagged specific cell types (INTACT) technology.	DESEQ2, fold change > 2 and FDR < 0.01.	WBPaper00062103:neuron_enriched
Bacteria infection: Pseudomonas aeruginosa PA14. 24 hours of exposure.	Small RNAs (21-26nt) that showed significantly increased expression after L4 animals were exposed to P .aeruginosa strain PA14 for 24 hours.	DESeq2, FDR < 0.05	WBPaper00056868:P.aeruginosa_upregulated_smallRNA
	Potental DAF-12 target genes identified by ChIP-chip analysis performed on strain ALF4 [daf-12	Affymetrix TAS software that computed for each probe estimates of fold enrichment (in linear scale) over hybridization with input DNA. At the same time, TAS calculated for each probe a p-value by applying a Wilcoxon signed rank test. A threshold of 2.5 was selected, which corresponds to probe intensities approximately 2.5 times stronger on the ChIP array than on the Input array. Additional TAS threshold parameters were MinRun=180 bp, MaxGap=300 bp. TAS analysis showed that the selected threshold of 2.5 corresponds approximately to a p-value of 0.01.	WBPaper00040221:DAF-12_target_ALF4
	Transcripts that showed significantly increased expression in VC1743[mett-10(ok2204)] comparing to in N2 when animals grow on standard (OP50) plates.	DESeq2, p-value < 0.05, fold change > 2.	WBPaper00061357:mett-10(ok2204)_upregulated_OP50
	MicroRNAs that showed significantly decreased expression in alg-5(ram2), comparing to in N2.	DESeq2, Fold change > 1.5.	WBPaper00051404:alg-5(ram2)_downregulated_miRNA
Fasting for 48 hours, from 3 days post L4 to 5 days post L4.	MicroRNAs that showed significantly decreased expression after 48 hours of fasting	p < 0.0002, Fishers exact test.	WBPaper00051247:fasting_downregulated
	miRNAs that showed increased expression in 1 day post L4 adult hermaphrodite eat-2(ad1116) comparing to in N2.	A fold change >= 1.5 with a minimum read count of >= 10 were used to filter the differentially expressed miRNA. The p-value cutoff was set at p <= 0.05 based on Kals Z test statistical.	WBPaper00046156:eat-2(ad1116)_Day1_upregulated
	miRNA with increased expression in N2 1-day post L4 adult hermaphrodite comparing to in N2 L4 larva.	DEGseq and GFOLD were used to analyze miRNAs expression. Authors chose miRNAs which had more than two-fold difference in expression (P < 0.001, q < 0.01 of Storey) from DEGseq, and miRNAs which had more than two-fold difference in expression (GFOLD score > 0 for up-regulation and GFOLD score < 0 for down-regulation) from GFOLD outcomes. Then authors obtained the intersection of up-regulated miRNAs and down-regulated miRNAs for each stage from the chosen miRNAs, respectively.	WBPaper00045316:miRNA_N2_adult_vs_L4_upregulated_adult
	Micro RNAs that showed significantly decreased expression in hrpk-1(zen17) comparing to in N2 at embryo stage.	DESeq, fold change > 2, p-value <= 0.05.	WBPaper00058673:hrpk-1(zen17)_downregulated_miRNA_embryo
	MicroRNAs that showed significantly decreased expression in alg-1(gk214), comparing to in N2.	DESeq2, Fold change > 1.5.	WBPaper00051404:alg-1(gk214)_downregulated_miRNA
	MicroRNAs that showed significantly increased expression in alg-2(ok304), comparing to in N2.	DESeq2, Fold change > 1.5.	WBPaper00051404:alg-2(ok304)_upregulated_miRNA
	miRNA with decreased expression in prg-1(wm161) comparing to in N2.	DEGseq and GFOLD were used to analyze miRNAs expression. Authors chose miRNAs which had more than two-fold difference in expression (P < 0.001, q < 0.01 of Storey) from DEGseq, and miRNAs which had more than two-fold difference in expression (GFOLD score > 0 for up-regulation and GFOLD score < 0 for down-regulation) from GFOLD outcomes. Then authors obtained the intersection of up-regulated miRNAs and down-regulated miRNAs for each stage from the chosen miRNAs, respectively.	WBPaper00045316:miRNA_prg-1_downregulated_adult
	MicroRNAs that showed significantly decreased expression in alg-5(tm1163), comparing to in N2.	DESeq2, Fold change > 1.5.	WBPaper00051404:alg-5(tm1163)_downregulated_miRNA
Heat Shock: 35C 4 hours at L4 larva stage.	Micro RNAs that showed significantly decreased expression after L4 larva N2 animals were heat stressed at 35C for 4 hours	DESeq2	WBPaper00057154:HeatShock_downregulated_miRNA
	miRNAs with age dependent abundance in wild type animals.	Details available in Supplementary material of the paper. Diff Exp, is the approximate ratio of expression comparing Young to Old animals (11 day difference in age). The raw p-value is for the null hypothesis that the Diff exp Ratio = 1 (based on robust inference - see supplemental information) and the associated 95% CI of the ratio.	WBPaper00042527:age_dependent_miRNA
	MicroRNAs that showed significantly increased expression in alg-5(ram2), comparing to in N2.	DESeq2, Fold change > 1.5.	WBPaper00051404:alg-5(ram2)_upregulated_miRNA
	MicroRNAs that showed significantly increased expression in mir-71(n4115) comparing to in N2 at 4-days post L4 adult hermaphrodite.	Differential expression for both small RNA- and mRNA-seq data was tested using DESeq2; P-values were adjusted for multiple testing by Benjamini-Hochberg method.	WBPaper00053318:mir-71(n4115)_upregulated_miRNA
Bacteria infection: Pseudomonas aeruginosa PA14	miRNAs up regulated by Pseudomonas aeruginosa infection.	N.A.	WBPaper00046303:P.aeruginosa_upregulated_miRNA
	miRNA with increased expression in N2 L1 larva comparing to in N2 embryo.	DEGseq and GFOLD were used to analyze miRNAs expression. Authors chose miRNAs which had more than two-fold difference in expression (P < 0.001, q < 0.01 of Storey) from DEGseq, and miRNAs which had more than two-fold difference in expression (GFOLD score > 0 for up-regulation and GFOLD score < 0 for down-regulation) from GFOLD outcomes. Then authors obtained the intersection of up-regulated miRNAs and down-regulated miRNAs for each stage from the chosen miRNAs, respectively.	WBPaper00045316:miRNA_N2_L1_vs_embryo_upregulated_adult
	MicroRNAs that showed significantly increased expression in alg-1(gk214), comparing to in N2.	DESeq2, Fold change > 1.5.	WBPaper00051404:alg-1(gk214)_upregulated_miRNA
space radition	Micro RNAs that showed increased expression in space flight control N2 samples (animals were put in a centrifugal device in space) comparing to in ground control, affected by space radition but not microgravity.	Differentially expressed miRNAs (>1.5-fold change and <0.67) and mRNAs (>2-fold change and <0.5) were identified through fold-change filtering.	WBPaper00048474:SpaceRadiation_upregulated_N2
	miRNA that showed decreased expression after 20mM nicotine treatment	Statistical analysis was based on t-test for independent samples via SPSS. The results were further narrowed based on two criteria. Only the genes whose expression changed with a P < 0.05 and a fold change > 0.5, when compared to control, were considered as differentially expressed. Fold change was calculated as 2**(DeltaCt(treatment)-DeltaCt(control))-1. DeltaCt = Ct(miRNA)-Ct(aveMRNA).	WBPaper00042480:20mM_nicotine_downregulated

4 Expression Patterns

• Primary Identifier: Expr9258
• Pattern: miR-54-56 GFP reporter was restricted in expression to anterior and ventral cells identified as neurons, tail hypodermal cells, and cells of the excretory system.

• Primary Identifier: Expr8594
• Remark: Picture: Fig. 4A-D.
• Pattern: mir-54/55/56::gfp was detected in the cell nuclei of essentially all somatic cells when driven by a region extending 6.0 kb upstream from the mir-54 coding sequence. Expression was first observed in the 28-cell stage embryo, weakly and diffusely within nuclei of all somatic cells. The expression then became strong from 200-cell stage onward and persisted throughout embryogenesis, larval stages and adulthood.
• Subcellular Localization: Expressed weakly and diffusely within nuclei of all somatic cells.

• Primary Identifier: Expr2485
• Pattern: Intense signals were detected in embryos, larval stages (L1, L2, L3, L4), adults, and glp-4(bn2) adults.

• Primary Identifier: Expr10910

0 GO Annotation

0 Homologues

1 Locations

• Feature . Primary Identifier: WBGene00003283
• Start: 13144827
• End: 13144924
• Strand: -1

0 Ontology Annotations

0 Regulates Expr Cluster

1 Sequence

• Length: 98

1 Sequence Ontology Term