2 Genes
WormBase Gene ID | Gene Name | Sequence Name | Organism |
---|---|---|---|
WBGene00001864 | him-5 | D1086.4 | Caenorhabditis elegans |
WBGene00004010 | pha-1 | Y48A6C.5 | Caenorhabditis elegans |
WormBase ID | WBStrain00024074 | CGC Received | 2014-04-22 |
Genotype | pha-1(e2123) III; him-5(e1490) V; rnyEx109. | Laboratory | CGC |
Made By | WBPerson2233 | Name | KWN190 |
Remark | rnyEx109 [nhx-2p::D3cpv + pha-1(+)]. Maintain at 20-25C to select for array. KWN190 strain expresses the FRET-based calcium indicator protein D3cpv throughout the cytoplasm of intestinal cells. Dual emission ratio imaging (ex. 435, em. 480/535-nm) can be used to measure intestinal calcium and, although the FRET pair is CFP/YFP, intestinal D3cpv fluorescence is observable under standard GFP filter sets. The D3cpv biosensor has the advantages of being relatively pH-insensitive and not interfering with endogenous calmodulin signaling. References: Am J Physiol Cell Physiol. 301:C1389-1403. Am J Physiol Cell Physiol. 297:C1071-81. FASEB J. 27:760-768. PLoS Biol. 11: e1001613. | Species | Caenorhabditis elegans |
WormBase Gene ID | Gene Name | Sequence Name | Organism |
---|---|---|---|
WBGene00001864 | him-5 | D1086.4 | Caenorhabditis elegans |
WBGene00004010 | pha-1 | Y48A6C.5 | Caenorhabditis elegans |