1 Genes
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00006706 | ubc-9 | F29B9.6 | Caenorhabditis elegans |
| WormBase ID | WBStrain00034466 | CGC Received | 2018-05-09 |
| Genotype | ubc-9(iow31[3xFLAG::ubc-9]) IV/nT1[qIs51] (IV;V) | Laboratory | CGC |
| Mutagen | CRISPR_Cas9 | Name | SSM291 |
| Outcrossed | x2 | Remark | Heterozygotes are wild type with pharyngeal-expressed GFP, and segregate wild type GFP+ heterozygotes, arrested nT1[qIs51] homozygotes, and viable non-GFP ubc-9(iow31[3xFLAG::ubc-9]) homozygotes. ubc-9(iow31[3xFLAG::ubc-9]) homozygotes produce 100% dead embryos, but cytologically they have normal germline (examined by DAPI and RAD-51 staining), suggesting that 3xFLAG::ubc-9 is functional in the gonad but not in the non-maternally rescued embryo. Maintain the strain by picking GFP+ worms. 3XFLAG bas added by CRISPR/Cas9. Synonymous point mutation where included in the repair template (positions 30, 33 and 36 of coding sequence). Reference: Reichman R, et al. Genetics. 2018 Apr;208(4):1421-1441. |
| Species | Caenorhabditis elegans |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00006706 | ubc-9 | F29B9.6 | Caenorhabditis elegans |
