WormMine

WS295

Intermine data mining platform for C. elegans and related nematodes

Strain :

WormBase ID  WBStrain00047706 CGC Received  2019-05-24
Genotype  pcn-1(am315[3xFLAG]) IV/nT1 [qIs51] (IV;V). Laboratory  CGC
Made By  WBPerson33092 Mutagen  CRISPR_Cas9
Name  WU1854 Outcrossed  x1
Remark  Homozygous maternal effect lethal mutation balanced by GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP am315 homozygotes (maternal effect lethal). Homozygous nT1[qIs51] inviable. Pick wild-type GFP and check for correct segregation of progeny to maintain. 3xFLAG epitope allows antibody detection of full-length PCN-1. Reference: Kocsisova Z, et al. BMC Dev Biol. 2018 May 30;18(1):12. Species  Caenorhabditis elegans

1 Alleles

Public Name
am315

1 Data Sets

Name URL
WormBaseAcedbConverter  

1 Genes

WormBase Gene ID Gene Name Sequence Name Organism
WBGene00003955 pcn-1 W03D2.4 Caenorhabditis elegans