1 Genes
WormBase Gene ID | Gene Name | Sequence Name | Organism |
---|---|---|---|
WBGene00003955 | pcn-1 | W03D2.4 | Caenorhabditis elegans |
WormBase ID | WBStrain00047706 | CGC Received | 2019-05-24 |
Genotype | pcn-1(am315[3xFLAG]) IV/nT1 [qIs51] (IV;V). | Laboratory | CGC |
Made By | WBPerson33092 | Mutagen | CRISPR_Cas9 |
Name | WU1854 | Outcrossed | x1 |
Remark | Homozygous maternal effect lethal mutation balanced by GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP am315 homozygotes (maternal effect lethal). Homozygous nT1[qIs51] inviable. Pick wild-type GFP and check for correct segregation of progeny to maintain. 3xFLAG epitope allows antibody detection of full-length PCN-1. Reference: Kocsisova Z, et al. BMC Dev Biol. 2018 May 30;18(1):12. | Species | Caenorhabditis elegans |
WormBase Gene ID | Gene Name | Sequence Name | Organism |
---|---|---|---|
WBGene00003955 | pcn-1 | W03D2.4 | Caenorhabditis elegans |