3 Genes
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00006843 | unc-119 | M142.1 | Caenorhabditis elegans |
| WBGene00009163 | drsh-1 | F26E4.10 | Caenorhabditis elegans |
| WBGene00011908 | pash-1 | T22A3.5 | Caenorhabditis elegans |
| WormBase ID | WBStrain00050761 | CGC Received | 2020-10-21 |
| Genotype | drsh-1(luc82[myc::AID::3XFLAG::4xGGSG::drsh-1::4xGGSG::3xFLAG::AID::myc]) pash-1(luc71[pash-1::2xGGSG::3xFLAG::AID::myc]) I; ieSi57 II; unc-119(ed3) III; ieSi38 IV; lucIs20; lucIs24. | Laboratory | CGC |
| Mutagen | CRISPR_Cas9 | Name | MLC1729 |
| Outcrossed | x0 | Remark | ieSi57 [eft-3p::TIR1::mRuby::unc-54 3'UTR + Cbr-unc-119(+)] II. ieSi38 [sun-1p::TIR1::mRuby::sun-1 3'UTR + Cbr-unc-119(+)] IV. lucIs20 [mir-35p::mirtron-35 + myo-2::mCherry]. lucIs24 [mir-52p::mirtron-51 + elt-2::dsRed + myo-2::mCherry]. Endogenous drsh-1 tagged at both N- and C-termini with the auxin-inducible-degron (AID) peptide. Endogenous pash-1 tagged with the AID peptide at the C-terminus. Strain expresses modified Arabidopsis thaliana TIR1 tagged with mRuby in soma and germline. In addition, strain expresses mirtron-versions of mir-35 and mir-51, which are processed independently of Drosha and Pasha. miRNA biogenesis can be stringently inhibited via simultaneous removal of Drosha and Pasha, causing absence of all canonical miRNAs and embryonic lethality upon Auxin treatment. Reference: Dexheimer, PJ, et al. Curr Biol. 2020. in press. |
| Species | Caenorhabditis elegans |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00006843 | unc-119 | M142.1 | Caenorhabditis elegans |
| WBGene00009163 | drsh-1 | F26E4.10 | Caenorhabditis elegans |
| WBGene00011908 | pash-1 | T22A3.5 | Caenorhabditis elegans |
