WormMine

WS295

Intermine data mining platform for C. elegans and related nematodes

Strain :

WormBase ID  WBStrain00050646 CGC Received  2021-01-20
Genotype  goa-1(n499)/tmC20 [unc-14(tmIs1219) dpy-5(tm9715)] I. Laboratory  CGC
Made By  WBPerson2207 Name  CGC140
Outcrossed  x2 Remark  Homozygous lethal mutation balanced by Dpy- and myo-2p::Venus-marked inversion. Heterozygotes are paralyzed Unc and Egl with relatively dim pharyngeal GFP (Venus) expression. Heterozygotes segregate heterozygous non-Dpy GFP+ paralyzed Unc and Egl, non-GFP embryonic lethal (homozygous n499), and Dpy with brighter GFP+ (tmC20 homozygous). This strain is difficult and time consuming to maintain. Gives relatively few heterozygotes. Remove Dpy from plate to prevent them from taking over. Heterozygotes tend to stack up in parallel clumps. Populations can be enriched by transferring these clumps to new plates and allowing Dpy (tmC20 homozygotes) to crawl out into bacterial lawn, and then picking away Dpy or transferring the clump of Hets to another plate. Derived by balancing n499 from parental strain MT1102 over tmC20 from FX30179.
Species  Caenorhabditis elegans

2 Alleles

Public Name
n499
tm9715

1 Data Sets

Name URL
WormBaseAcedbConverter  

3 Genes

WormBase Gene ID Gene Name Sequence Name Organism
WBGene00001067 dpy-5 F27C1.8 Caenorhabditis elegans
WBGene00001648 goa-1 C26C6.2 Caenorhabditis elegans
WBGene00006753 unc-14 K10D3.2 Caenorhabditis elegans