2 Genes
WormBase Gene ID | Gene Name | Sequence Name | Organism |
---|---|---|---|
WBGene00021912 | affl-2 | Y55B1BR.2 | Caenorhabditis elegans |
WBGene00021911 | affl-1 | Y55B1BR.1 | Caenorhabditis elegans |
WormBase ID | WBStrain00050897 | CGC Received | 2021-02-18 |
Genotype | affl-2(sy975) Y55B1BR.1(sy1220) III. | Laboratory | CGC |
Mutagen | CRISPR_Cas9 | Name | PS8203 |
Outcrossed | xNo | Remark | Superficially wild-type. CRISPR/Cas9 engineered STOP-IN null mutant of Y55B1BR.1 into sup-45 mutant (sy975);Universal 43bp-long knock-in insertion with 3-frame stop codon (STOP-IN cassette).Left flanking sequence: CCGTACCCGTAGAATGCTTGAAGAAATGGCCGGCCRight flanking sequence: TCGTGGGAACTAAACCATTGAGCCAGCTTCCTCGAAGinserted sequence between the two flanking sequence (STOP-In casette): GGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAgctagc. sgRNA : TGAAGAAATGGCCGGCCTCGMethod Reference: G3 (Bethesda). 2018 Nov 6;8(11):3607-3616 |
Species | Caenorhabditis elegans |
WormBase Gene ID | Gene Name | Sequence Name | Organism |
---|---|---|---|
WBGene00021912 | affl-2 | Y55B1BR.2 | Caenorhabditis elegans |
WBGene00021911 | affl-1 | Y55B1BR.1 | Caenorhabditis elegans |