3 Genes
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00004027 | pie-1 | Y49E10.14 | Caenorhabditis elegans |
| WBGene00004895 | smu-1 | CC4.3 | Caenorhabditis elegans |
| WBGene00006537 | tbb-2 | C36E8.5 | Caenorhabditis elegans |
| WormBase ID | WBStrain00051046 | CGC Received | 2021-02-04 |
| Genotype | he314[pie-1p::GLO-ePDZ::mCherry::smu-1::tbb-2 3'UTR] II; e259[eft-3p::PH::eGFP::LOV::tbb-2 3'UTR]) IV. | Laboratory | CGC |
| Made By | WBPerson21977 | Mutagen | CRISPR_Cas9 |
| Name | SV2061 | Outcrossed | x0 |
| Remark | he314[pie-1p::GLO-ePDZ::mCherry::smu-1::tbb-2 3'UTR] II. e259[eft-3p::PH::eGFP::LOV::tbb-2 3'UTR]) IV. Superficially wild-type. CRISPR/Cas9 was used to create insertion alleles he314 and he259 insertions into N2 background at sites of known MosSCI insertions ttTi5605 and cxTi10816, respectively. ePDZLOV system transgenes allow use of blue light to control protein heterodimerization, in this case, membrane recruitment of ePDZ-tagged proteins of interest. Germline-optimized cytosolic ePDZ::mCherry-tagged SMU-1 (GLO-ePDZ::mCherry::SMU-1), and membrane-bound LOV2 domain fused to a pleckstrin-homology domain (PH::eGFP::LOV). GLO-ePDZ::mCherry is a germline-optimized variant coded to be less prone to silencing in the germline. Reference: Fielmich LE, et al. eLife 2018 Aug 15;7:e38198. doi: 10.7554/eLife.38198. | Species | Caenorhabditis elegans |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00004027 | pie-1 | Y49E10.14 | Caenorhabditis elegans |
| WBGene00004895 | smu-1 | CC4.3 | Caenorhabditis elegans |
| WBGene00006537 | tbb-2 | C36E8.5 | Caenorhabditis elegans |
