2 Genes
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00003639 | nhr-49 | K10C3.6 | Caenorhabditis elegans |
| WBGene00007016 | mdt-15 | R12B2.5 | Caenorhabditis elegans |
| WormBase ID | WBStrain00051874 | CGC Received | 2021-09-16 |
| Genotype | nhr-49(et8) I; mdt-15(et14) III. | Laboratory | CGC |
| Made By | WBPerson11984 | Mutagen | EMS |
| Name | QC155 | Outcrossed | x10 |
| Remark | This double mutant strain contains an excess polyunsaturated fatty acids in its cell membranes accompanied by excess lipid peroxidation, cell permeability, increased autophagy and other defects. The nhr-49(et8) C9873765T [WS200] mutation can be detected by PCR using the following primers: nhr-49 Fwd: 5-CAGATTATGATTCGTGATGCTAGA-3; nhr-49 WT Rev: 5-GAGATGAAAGATGTTGCTGTAGAG-3; nhr-49 Mut Rev: 5-GAGATGAAAGATGTTGCTGTAGAA-3. Annealing 65C, expected products ~300 bp. The mdt-15(et14) C5832666T [WS200] mutation can be detected by PCR using the following primers: mdt-15(et14) Mut Fwd: 5-GTGCCTCCAGATCCACAGCT-3; mdt-15(et14) WT Fwd: 5-GTGCCTCCAGATCCACAGCC-3; mdt-15 Rev: 5-CACCCATTGGAGCACCACT-3. Annealing 65C, expected product ~400 bp. Reference: Devkota R, et al. Genetics (in press). Volume 219, Issue 1, September 2021. https: | Species | Caenorhabditis elegans |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00003639 | nhr-49 | K10C3.6 | Caenorhabditis elegans |
| WBGene00007016 | mdt-15 | R12B2.5 | Caenorhabditis elegans |
