2 Genes
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00001170 | egl-1 | F23B12.9 | Caenorhabditis elegans |
| WBGene00003263 | mir-35 | Y62F5A.2 | Caenorhabditis elegans |
| WormBase ID | WBStrain00051757 | CGC Received | 2022-02-22 |
| Genotype | mir-35(cdb2 cdb4) II; egl-1(cdb97) V. | Laboratory | CGC |
| Made By | WBPerson26844 | Mutagen | CRISPR_Cas9 |
| Name | MCJ217 | Outcrossed | x2 |
| Remark | Superficially wild type. Seed mutation of mir-35 was made by two rounds of CRISPR/Cas9 editing. cdb2 is a 50 bp deletion of the mir-35 locus. cdb4 was created by successive homology-directed repair of the disrupted mir-35 locus with a protospacer to preserve the secondary structure of the primary and precursor hairpin. egl-1(cdb97) contains engineered mutations in mir-35 binding site in the 3UTR region making the sequence complementary to the mir-35(cdb4) variant. Reference: Yang B, et al. Genes Dev. 2020 Sep 1;34(17-18):1227-1238. PMID: 32820039 | Species | Caenorhabditis elegans |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00001170 | egl-1 | F23B12.9 | Caenorhabditis elegans |
| WBGene00003263 | mir-35 | Y62F5A.2 | Caenorhabditis elegans |
