3 Genes
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00000898 | daf-2 | Y55D5A.5 | Caenorhabditis elegans |
| WBGene00000912 | daf-16 | R13H8.1 | Caenorhabditis elegans |
| WBGene00006843 | unc-119 | M142.1 | Caenorhabditis elegans |
| WormBase ID | WBStrain00051792 | CGC Received | 2021-10-19 |
| Genotype | daf-16(hq389[daf-16::gfp::degron]) I; daf-2(e1370) unc-119(ed3) III; hqSi12 IV. | Laboratory | CGC |
| Mutagen | CRISPR_Cas9 | Name | MQD2495 |
| Outcrossed | x0 | Remark | hqSi12 [eak-4p::TIR-1:mRuby::unc-54 3' UTR + Cbr-unc-119(+)] IV. Maintain at 15C. GFP tag and degron inserted at the 3' end of the endogenous daf-16 gene locus by CRISPR/Cas9 engineering. hqSi12 was generated by replacing the sun-1 promoter and 3' UTR of the ieSi38 insertion (cxTi10882 site) with the eak-4 promoter and unc-54 3'UTR using CRISPR/Cas9. This strain can be used for auxin-inducible degradation (AID) of DAF-16 protein in the XXX cells. hqSi12 previously known as hq388. Reference: Zhang Y, et al. BioRxiv. 2021 Aug 2. 2007.2031.454567. https: |
| Species | Caenorhabditis elegans |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00000898 | daf-2 | Y55D5A.5 | Caenorhabditis elegans |
| WBGene00000912 | daf-16 | R13H8.1 | Caenorhabditis elegans |
| WBGene00006843 | unc-119 | M142.1 | Caenorhabditis elegans |
