2 Genes
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00001086 | dpy-27 | R13G10.1 | Caenorhabditis elegans |
| WBGene00001867 | him-8 | T07G12.12 | Caenorhabditis elegans |
| WormBase ID | WBStrain00051718 | CGC Received | 2022-03-10 |
| Genotype | wrdSi3 II; dpy-27(xoe41[dpy-27::AID::myc]) III; him-8(me4) IV. | Laboratory | CGC |
| Mutagen | CRISPR_Cas9 | Name | JEL1197 |
| Outcrossed | x4 | Remark | wrdSi3 [sun-1p::TIR1::F2A::mTagBFP2::AID*::NLS::tbb-2 3'UTR] (II:0.77). Endogenous dpy-27 locus tagged with AID element and myc to create a conditional allele using the auxin-inducible degron (AID). In absence of auxin, ~40% of worms are male due to him-8(me4) mutation. In the presence of auxin, ~100% of worms are male (hermaphrodite-lethal due to degradation of AID-tagged DPY-27). Reference: Li Q, et al. Inducible degradation of dosage compensation protein DPY-27 facilitates isolation of Caenorhabditis elegans males for molecular and biochemical analyses. bioRxiv 2022.01.27.478040; doi: https: |
| Species | Caenorhabditis elegans |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00001086 | dpy-27 | R13G10.1 | Caenorhabditis elegans |
| WBGene00001867 | him-8 | T07G12.12 | Caenorhabditis elegans |
