1 Genes
WormBase Gene ID | Gene Name | Sequence Name | Organism |
---|---|---|---|
WBGene00004143 | pqn-59 | R119.4 | Caenorhabditis elegans |
WormBase ID | WBStrain00051697 | CGC Received | 2022-04-27 |
Genotype | pqn-59(utx51[mNG::3xFlag::pqn-59]) I. | Laboratory | CGC |
Mutagen | CRISPR_Cas9 | Name | GLW63 |
Outcrossed | x0 | Remark | N-terminal tag of PQN-59 via CRISPR/Cas9 knock-in of mNeonGreen at pqn-59 locus. Insertion verified by PCR and fluorescence. Left flank: 5' gaccctttttatttataatttgttttcaga 3'; Right flank: 5' ATGGGTATTAAAGGCGACAAAAAAGCTACT 3 (1 silent mutation); sgRNA: TGCAAGTCGCGCTTGATCGC; Cas9/sgRNA plasmid: pGLOW23; mNG^SEC^3xFlag plasmid: pGLOW24; SEC insertion allele strain (balanced): GLW62. |
Species | Caenorhabditis elegans |
WormBase Gene ID | Gene Name | Sequence Name | Organism |
---|---|---|---|
WBGene00004143 | pqn-59 | R119.4 | Caenorhabditis elegans |