1 Genes
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00002008 | hsp-4 | F43E2.8 | Caenorhabditis elegans |
| WormBase ID | WBStrain00051694 | CGC Received | 2022-01-24 |
| Genotype | hsp-4(utx39[hsp-4::mNG::3xFlag]) II. | Laboratory | CGC |
| Mutagen | CRISPR_Cas9 | Name | GLW47 |
| Outcrossed | x0 | Remark | C-terminal tag of HSP-4 via CRISPR/Cas9 knock-in of mNeonGreen at hsp-4 locus. Insertion verified by PCR and fluorescence. Left flank: 5' TCGGCCGGAGGACAAGGAGAACAAGCTTCTGAGGAGCCATCGGAGGATCATGATGAACTG 3' (1 silent mutation); Right flank: 5' TAAaatattaattgccttcaactacttgct 3'; sgRNA: CGTCTCCAAACTTTACTCGG; Cas9/sgRNA plasmid: pGLOW44; mNG^SEC^3xFlag plasmid: pGLOW61; SEC insertion allele strain: GLW46. |
| Species | Caenorhabditis elegans |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00002008 | hsp-4 | F43E2.8 | Caenorhabditis elegans |
