1 Genes
WormBase Gene ID | Gene Name | Sequence Name | Organism |
---|---|---|---|
WBGene00011036 | edc-3 | R05D11.8 | Caenorhabditis elegans |
WormBase ID | WBStrain00051692 | CGC Received | 2022-01-24 |
Genotype | edc-3(utx35[mNG::3xFlag::edc-3]) I. | Laboratory | CGC |
Mutagen | CRISPR_Cas9 | Name | GLW43 |
Outcrossed | x0 | Remark | N-terminal tag of EDC-3 via CRISPR/Cas9 knock-in of mNeonGreen at edc-3 locus. Insertion verified by PCR and fluorescence. Left flank: 5' ggttccaattcttctgatttcaaattaaaa 3'; Right flank: 5' ATGGATGACAAACTCATTGGAAGCGTCATCTCTACGGAGACAAAGGACGG 3' (7 silent mutations); sgRNA: ATATCAACCGAAACTAAAGA; Cas9/sgRNA plasmid: pGLOW81; mNG^SEC^3xFlag plasmid: pGLOW103; SEC insertion allele strain: GLW42. |
Species | Caenorhabditis elegans |
WormBase Gene ID | Gene Name | Sequence Name | Organism |
---|---|---|---|
WBGene00011036 | edc-3 | R05D11.8 | Caenorhabditis elegans |