2 Genes
WormBase Gene ID | Gene Name | Sequence Name | Organism |
---|---|---|---|
WBGene00006784 | unc-49 | T21C12.1 | Caenorhabditis elegans |
WBGene00013422 | popl-5 | Y66A7A.2 | Caenorhabditis elegans |
WormBase ID | WBStrain00052062 | CGC Received | 2022-03-30 |
Genotype | popl-5(udn109)/tmC29 [unc-49(tmIs1259)] III. | Laboratory | CGC |
Mutagen | CRISPR_Cas9 | Name | UDN100215 |
Outcrossed | x2 | Remark | popl-5 [S99S]/tmC29 III. Control edit mutation maintained over tmC29. Balancer marked with myo-2p::GFP. Heterozygotes are WT with pharyngeal GFP, and segregate GFP+ heterozygotes, non-GFP popl-5 [S99S] homozygotes (viable and fertile), and Unc GFP+ tmC29 homozygotes. Pick fertile wild-type GFP+ to maintain. NOTE: udn109 is essentially wild-type. Pick GFP+ to prevent non-GFP popl-5 [S99S] homozygotes from taking over the population and losing the balancer! Silent AvaII site added in S99S allele for ease of genotyping. Reference: Huang et al. 2022. PMID: 35121658 |
Species | Caenorhabditis elegans |
WormBase Gene ID | Gene Name | Sequence Name | Organism |
---|---|---|---|
WBGene00006784 | unc-49 | T21C12.1 | Caenorhabditis elegans |
WBGene00013422 | popl-5 | Y66A7A.2 | Caenorhabditis elegans |