2 Genes
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00001072 | dpy-10 | T14B4.7 | Caenorhabditis elegans |
| WBGene00001511 | fzy-1 | ZK177.6 | Caenorhabditis elegans |
| WormBase ID | WBStrain00054967 | CGC Received | 2023-02-14 |
| Genotype | fzy-1(lt20::loxP)/mIn1[mIs14 dpy-10(e128)] II. | Laboratory | CGC |
| Made By | WBPerson29989 | Mutagen | CRISPR_Cas9 |
| Name | OD2359 | Outcrossed | x6 |
| Remark | CRISPR/Cas9 engineered deletion of fzy-1 in which the fzy-1 coding sequence was replaced by LoxP. Homozygous lethal deletion chromosome balanced by GFP- and dpy-10-marked inversion. Heterozygotes are wild-type with relatively dim pharyngeal GFP signal, and segregate wild-type dim GFP (heterozygotes), Dpy bright GFP (mIn1 homozygotes), and non-GFP fzy-1 homozygotes (larval arrest). Pick wild-type with dim GFP and check for correct segregation of progeny to maintain. gRNA sequence: Ggacgcacgcccggtagtgc Reference: Kim T, et al. Genes Dev. 2017 Jun 1;31(11):1089-1094. doi: 10.1101/gad.302067.117. PMID: 28698300. | Species | Caenorhabditis elegans |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00001072 | dpy-10 | T14B4.7 | Caenorhabditis elegans |
| WBGene00001511 | fzy-1 | ZK177.6 | Caenorhabditis elegans |
