1 Genes
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00002246 | lag-2 | Y73C8B.4 | Caenorhabditis elegans |
| WormBase ID | WBStrain00054809 | CGC Received | 2023-09-11 |
| Genotype | lag-2(q1049[lag-2::3xV5]) V. | Laboratory | CGC |
| Made By | WBPerson123 | Mutagen | CRISPR_Cas9 |
| Name | JK6099 | Outcrossed | x2 |
| Remark | 3xV5 tag inserted at C-terminus of endogenous lag-2 locus. Hermaphrodite DTC stain well. Animals are fertile and look wild-type. tracr + crRNA targeting the C-terminus of LAG-2 was injected with Cas-9 protein and repair template to insert a 3xV5 tag, following Fire and Seydoux lab RNP co-CRISPR strategy (with unc-58 crRNA and repair oligo to mark edited offspring). | Species | Caenorhabditis elegans |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00002246 | lag-2 | Y73C8B.4 | Caenorhabditis elegans |
