2 Genes
WormBase Gene ID | Gene Name | Sequence Name | Organism |
---|---|---|---|
WBGene00000254 | bli-4 | K04F10.4 | Caenorhabditis elegans |
WBGene00003083 | lst-1 | T22A3.3 | Caenorhabditis elegans |
WormBase ID | WBStrain00054807 | CGC Received | 2023-09-29 |
Genotype | lst-1(q869) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). | Laboratory | CGC |
Mutagen | CRISPR_Cas9 | Name | JK5796 |
Outcrossed | x7 | Remark | Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP q869 homozygotes (viable and somewhat fertile). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. q869 is a deletion of the entire lst-1 coding sequence as well as 139 bp upstream of M71 start codon and 228 bp downstream of the coding sequence. Reference: Haupt KA, et al. Development. 2019 Oct 17;146(20):dev181644. doi: 10.1242/dev.181644. PMID: 31515205 |
Species | Caenorhabditis elegans |
WormBase Gene ID | Gene Name | Sequence Name | Organism |
---|---|---|---|
WBGene00000254 | bli-4 | K04F10.4 | Caenorhabditis elegans |
WBGene00003083 | lst-1 | T22A3.3 | Caenorhabditis elegans |