2 Genes
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00006925 | vit-1 | K09F5.2 | Caenorhabditis elegans |
| WBGene00006926 | vit-2 | C42D8.2 | Caenorhabditis elegans |
| WormBase ID | WBStrain00054880 | CGC Received | 2023-03-23 |
| Genotype | vit-2(ok3211) vit-1(hq532) X. | Laboratory | CGC |
| Mutagen | CRISPR_Cas9 | Name | MQD2884 |
| Outcrossed | x0 | Remark | hq532 is a CRISPR-engineered knockout of vit-1 in vit-2(ok3211) background removing 8 bp from the third exon of vit-1: WT sequence AAAGCATTGAGAAGGAGTCCACAACTGTTGTCCGCGGACGCCGTATCCAAACCGGAATCACG mutated to AAAGCATTGAGAAGGAGTCCACAAC--------GCGGACGCCGTATCCAAACCGGAATCACG. For genotyping, the following primers will produce ~800 bp DNA fragment that can be sequenced. Forward primer: TACCAACGTGTTGCTATCGTTTGCTC. Reverse primer: TTGCTCGAAGAGTGGGGTGAACATTCTC. Strain does not express vit-1 or vit-2. Reference: Zhai C, et al. bioRxiv 2022.06.27.497668; doi: https: |
| Species | Caenorhabditis elegans |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00006925 | vit-1 | K09F5.2 | Caenorhabditis elegans |
| WBGene00006926 | vit-2 | C42D8.2 | Caenorhabditis elegans |
