1 Genes
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00020246 | clic-1 | T05B11.3 | Caenorhabditis elegans |
| WormBase ID | WBStrain00054753 | CGC Received | 2023-01-10 |
| Genotype | clic-1(utx73[mScarlet-I-C1::3xMyc::clic-1]) V. | Laboratory | CGC |
| Mutagen | CRISPR_Cas9 | Name | GLW93 |
| Outcrossed | x0 | Remark | N-terminal tag of CLIC-1 via CRISPR/Cas9 knock-in of mScarlet at clic-1 locus. Insertion verified by PCR and fluorescence. Genotyping primers: fwd 5 CTCGTACCAATCGTCCGCAA 3; rev 5 CCATCTTGTTGCTTGGCGAAA 3. Left flank: 5' ccagggtataaaacacaaaaaaactacaaa 3'; Right flank: 5' ATGTCGGATCCAGTCGCGGATTTTTTGGCT 3 (1 silent mutation); sgRNA: ctacaaaATGTCGGATCCAG; Cas9/sgRNA plasmid: pGLOW128; mScarlet^SEC^3xMyc plasmid: pGLOW156; SEC insertion allele strain: GLW92. |
| Species | Caenorhabditis elegans |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00020246 | clic-1 | T05B11.3 | Caenorhabditis elegans |
