1 Genes
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00001999 | hrpa-1 | F42A6.7 | Caenorhabditis elegans |
| WormBase ID | WBStrain00054752 | CGC Received | 2023-01-10 |
| Genotype | hrpa-1(utx71[hrpa-1::mScarlet-I-C1::3xMyc]) IV. | Laboratory | CGC |
| Mutagen | CRISPR_Cas9 | Name | GLW91 |
| Outcrossed | x0 | Remark | C-terminal tag of HRPA-1 via CRISPR/Cas9 knock-in of mScarlet at hrpa-1 locus. Insertion verified by PCR and fluorescence. Genotyping primers: fwd 5 GTCTCCACCAAACGCCTGTA 3 ; rev 5 CTAGCCTGTGTCCCATCAGC 3. Left flank: 5' AATGGGCCCACGCCCAGGGAGGAAACAGAAACTAT 3' (5 silent mutations); Right flank: 5' TAAattaattccttaagcccctctaagtgt 3; sgRNA: CAGTGGGCTCATGCTCAAGG; Cas9/sgRNA plasmid: pGLOW144; mScarlet^SEC^3xMyc plasmid: pGLOW153; SEC insertion allele strain: GLW90. |
| Species | Caenorhabditis elegans |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00001999 | hrpa-1 | F42A6.7 | Caenorhabditis elegans |
