1 Genes
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00004882 | smg-4 | F46B6.3 | Caenorhabditis elegans |
| WormBase ID | WBStrain00055592 | CGC Received | 2023-08-23 |
| Genotype | smg-4(az152) V. | Laboratory | CGC |
| Made By | WBPerson713 | Mutagen | CRISPR_Cas9 |
| Name | SZ340 | Outcrossed | x1 |
| Remark | CRISPR/Cas9 engineered smg-4 null allele. smg-4(az152) allele is confirmed NMD-defective by both the presence of the protruding vulva phenotype and the accumulation of NMD-targeted isoforms. smg-4(az152) is easy to track in crosses by PCR and digestion with BstBI (see S1 text of Suzuki, et al. for sequence of allele) and essentially mimics ma116 in having a G->A mutation at the last base of intron 1. az152 also removes two bases of exon 2 and inserts 50nt in exon 2. Reference: Suzuki JMNGL, et al. PLoS Genet. 2022 Feb 10;18(2):e1010028. doi: 10.1371/journal.pgen.1010028. PMID: 35143478. | Species | Caenorhabditis elegans |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00004882 | smg-4 | F46B6.3 | Caenorhabditis elegans |
