2 Genes
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00000254 | bli-4 | K04F10.4 | Caenorhabditis elegans |
| WBGene00003796 | npp-10 | ZK328.5 | Caenorhabditis elegans |
| WormBase ID | WBStrain00055401 | CGC Received | 2023-08-02 |
| Genotype | npp-10(sy2071) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). | Laboratory | CGC |
| Mutagen | CRISPR_Cas9 | Name | PS9999 |
| Outcrossed | x0 | Remark | Homozygous sterile CRISPR null mutant balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP+, arrested hT2 aneuploids, and non-GFP sy2071 homozygotes (L2 or early arrested sterile). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP+ and check for correct segregation of progeny to maintain. CRISPR/Cas9 engineered STOP-IN null mutant of npp-10. Universal 43bp-long knock-in insertion with 3-frame stop codon (STOP-IN cassette). Left flanking sequence: CAATCAAAACAAAGGATTGTTTGGTCAGCCAGCC. Right flanking sequence: AATAACAGTGGAACTACTGGCCTTTTCGGGGCGGC. Inserted sequence between the two-flanking sequence (STOP-IN cassette): GGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAgctagc. sgRNA: GTAGTTCCACTGTTATTGGC. Method Reference: Wang H, et al. G3 (Bethesda). 2018 Nov 6;8(11):3607-3616. |
| Species | Caenorhabditis elegans |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00000254 | bli-4 | K04F10.4 | Caenorhabditis elegans |
| WBGene00003796 | npp-10 | ZK328.5 | Caenorhabditis elegans |
